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Hormones in the grains in relation to sink strength and postanthesis development of spikelets in rice

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Abstract

Inferior spikelets usually exhibit a slower grain filling rate and lower grain weight than superior spikelets in a rice (Oryza sativa L.) panicle. This study investigated whether the variations in grain filling between the two kinds of spikelets were attributed to their sink strength and whether the sink strength was regulated by the hormonal levels in the grains. Using two field-grown rice genotypes, the division rate of endosperm cells, hormonal levels in the grains, and grain weight of both superior and inferior spikelets were determined during the grain filling period. The results showed that superior spikelets had dominance over inferior spikelets in endosperm cell division rate and cell number, grain filling and grain weight. Changes in zeatin (Z) and zeatin riboside (ZR) contents paralleled and were very significantly correlated with the cell division rate and cell number. Cell division rate and the content of indole-3-acetic acid (IAA) in the grains were also significantly correlated. Gibberellin (GAs; GA1+ GA4) content of the grains was high but ABA levels were low at the early grain filling stage. ABA increased substantially during the linear phase of grain growth and was very significantly correlated with grain dry weight during this period. Application of kinetin at 2 through 6 days post anthesis (DPA) significantly increased cell number, while spraying ABA at 11 through 15 DPA significantly increased the grain filling rate. The results suggest that differences in sink strength are responsible for variations in grain filling between superior and inferior spikelets. Both cytokinins and IAA in the grains may mediate cell division in rice endosperm at early grain filling stages, and therefore regulate the sink size of the grain, whereas ABA content correlates with sink activity during the linear period of grain growth.

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Authors and Affiliations

  1. College of Agriculture, Yangzhou University, Yangzhou, Jiangsu 225009, China, 514 7979087; fax

    Jianchang Yang, Zhiqin Wang & Qingsen Zhu

  2. Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China

    Jianhua Zhang

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  1. Jianchang Yang
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Yang, J., Zhang, J., Wang, Z. et al. Hormones in the grains in relation to sink strength and postanthesis development of spikelets in rice. Plant Growth Regulation 41, 185–195 (2003). https://doi.org/10.1023/B:GROW.0000007503.95391.38

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  • DOI: https://doi.org/10.1023/B:GROW.0000007503.95391.38

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