Abstract
Gliadin composition has been analysed in 403 accessions of spelt wheat (Triticum aestivum ssp. spelta); 61 different patterns were found for the -gliadins, 44 for the -gliadins, 19 for the -gliadins and 15 for the -gliadins. A subset of 333 accessions belonging to fifty populations from Asturias, North of Spain, showed high levels of genetic variation (A = 3.89, P = 0.88, Ne = 3.35 and He = 0.553), indicating that 82.5% of the genetic variation was within populations, and only 18.5% among populations. Thirty-five of these populations presented more of five accessions, in this new subset the values of genetic variation were higher that those of fifty populations (A = 4.49, P = 0.91, Ne = 3.80 and He = 0.595). The genetic variation within populations was 59.7% of the total, and 40.3% among populations, which could be associated to fixation effects of some alleles by genetic drift.
Similar content being viewed by others
References
Auricchio S., De Ritis G., De Vincenzi M., Occorsio P. and Silano V. 1982. Effects of gliadin derived peptides from bread and durum wheats on small intestine cultures from rat fetus and coelic children. Pediatr. Res. 16: 1004.
Bushuk W. and Zillman R.R. 1978. Wheat cultivar identification by gliadin electrophoregram. I. Apparatus, method, and nomenclature. Can. J. Plant Sci. 58: 505–515.
Caballero L., Martin L.M. and Alvarez J.B. 2001. Allelic variation of the HMW glutenin subunits in Spanish accessions of spelt wheat (Triticum aestivum ssp. spelta L. em. Thell.). Theor. Appl. Genet. 103: 124–128.
Caballero L., Martín L.M. and Alvarez J.B. 2003. Intra-and interpopulation diversity for HMW glutenin subunits in Spanish spelt wheat. Genet. Resour. Crop Evol. (in press).
D'Antuono L.F. 1989. Il farro; aerali di coltivaciones, caractteris-tiche agronomiche, utilizzazione e prospecttive colturali. L'In-formatore Agrario 45: 49–57.
Harsch S., Gunther T., Kling Ch.I., Rozynek B. and Hesemann C.U. 1997. Characterization of spelt (Triticum spelta L.) forms by gel electrophoretic analysed of seed storage proteins. I. The gliadins. Theor. Appl. Genet. 94: 52–60.
Hegde S.G.,Valkoun J. and Waines J.G. 2000. Genetic diversity in wild wheats and goat grass. Theor. Appl. Genet. 101: 309–316.
Khan K., Hamada A.S. and Patek J. 1985. Polyacrylamide gel electrophoresis for wheat variety identification: effect of variables on gel properties. Cereal Chem. 62: 310–313.
Lafiandra D., Benedettelli S., Margiotta B., Spagnoletti-Zeuli P.L. and Porceddu E. 1990. Seed storage-proteins and wheat genetic resources. In: Srivastava J.P. and Damania A.-B. (eds), Wheat genetic resources: meeting diverse needs. Aleppo, Syria, pp. 73–87.
Marshall D.R. and Brown A.H.D. 1975. Optimum sampling strate-gies in genetic conservation. In: Frankel O.H. and Hawkes J.G. (eds), Crop genetic resources for today and tomorrow. Cambridge University Press, Cambridge, pp. 53–70.
Metakovski E.V., Novoselskaya A.Y., Kopus M.M., Sobko T.A. and Sozinov A.A. 1984. Blocks of gliadin components in winter wheat detected by one-dimensional polyacrylamide gel electro-phoresis. Theor. Appl. Genet. 67: 559–568.
Nei M. 1973. Analysis of gene diversity in subdivided populations. Proc. Natl. Acad. Sci. USA 70: 3321–3323.
Nevo E. and Beiles A. 1989. Genetic diversity of wild emmer wheat in Israel and Turkey. Structure, evolution and application in breeding. Theor. Appl. Genet. 77: 421–455.
Nevo E. and Payne P.I. 1987. Wheat storage proteins: diversity of HMW-glutenin subunit in wild emmer from Israel. 1. Geographical patterns and ecological predictability. Theor. Appl. Genet. 74: 827–836.
Padulosi S., Hammer K. and Heller J. 1996. Hulled wheats. Rome, International Plant Genetic Resources Institute.
Payne P.I. 1987. Genetics of wheat storage proteins and the effect of allelic variation on bread-making quality. Ann. Rev. Plant Physiol. 38: 141–153.
Payne P.I., Holt L.M., Lawrence G.J. and Law C.N. 1982. The genetics of gliadins and glutenin, the major storage proteins of the wheat endosperm. Qual. Plant Foods Hum. Nutr. 31: 229–241.
Pena-Chocarro L. and Zapata-Pena L. 1998. Hulled wheats in Spain: history of minor cereals. In: Jaradat A.A. (ed.), Triticeae III. Science Publisher Inc, pp. 45–52.
Pfluger L.A., Martýn L.M. and Alvarez J.B. 2001.Variation in the HMW and LMW glutenin subunits from Spanish accessions of emmer wheat (Triticum turgidum ssp. dicoccum Schrank). Theor. Appl. Genet. 102: 767–772.
Porceddu E., Ceoloni C., Lafiandra D., Tanzarella O.A. and Scaras-cia Magnozza G.T. 1988. Genetic resources and plant breeding: problems and prospects. Proc. 7th Int. Wheat Genet. Symp., pp. 7–21.
Romanova Yu.A., Gubareva N.K., Konarev A.V., Mitrofanova O.P., Lyapunova O.A., Anfilova N.A. et al. 2001. Analysis of gliadin polymorphism in a Triticum spelta L. collection. Rus. J. Genet. 37: 1054–1060.
Sozinov A.A. and Poperelya F.A. 1980. Genetic classification of prolamins and its use for plant breeding. Ann. Techno. Agric. 29: 229–245.
Strehlow W., Hertzka G. and Weuffen W. 1991. The dietary properties of spelt in the treatment of chronical diseases. In: Proc. 2. Hohenheimer Dinkel Kolloquium. Hohenheim, Alemania Verlag, Stuttgart, pp. 243–259.
Zillman R.R. and Bushuk W. 1979. Wheat cultivar identification by gliadin electropherograms. III. Catalogue of electropherogram formulas of Canadian wheat cultivars. Can. J. Plant Sci. 59: 287–298.
Wright S. 1965. The interpretation of population by F-statistics with special regard to systems of matings. Evolution 19: 395–420.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Caballero, L., Martín, L. & Alvarez, J. Variation and genetic diversity for gliadins in Spanish spelt wheat accessions. Genetic Resources and Crop Evolution 51, 679–686 (2004). https://doi.org/10.1023/B:GRES.0000034575.97497.6e
Issue Date:
DOI: https://doi.org/10.1023/B:GRES.0000034575.97497.6e