Abstract
RFLP molecular markers have been used for the identification of 12 commercial varieties of tall fescue (Festuca arundinacea Schreb.). Of these, 7 could be distinguished on the basis of the presence/absence of specific and unique bands whereas the other 5 could be distinguished on the basis of band combinations. No differences in the RFLP pattern were detected between accessions of the same variety, whether obtained from the same source or from different sources. Bulked DNA probes from 100 plants are recommended as representative sample for allogamic crops. The use of molecular markers for seed certification in tall fescue is discussed.
This is a preview of subscription content, log in via an institution to check access.
References
Abernethy R.H., Steiner J.J., Wofforfd D.S. and Thiel D.S. 1989. Classification and pedigree verification of tall fescue cultivars utilizing the prolamine seed protein fraction. Crop Sci. 29: 791-797.
Caceres M.E., Pupilli F., Piano E. and Arcioni S. 2000. RFLP markers are an effective tool for the identification of creeping bentgrass (Agrostis stolonifera L.) cultivars. Genet. Resour. & Crop Evol. 47: 455-459.
Caetano-Anollés G. 1998. DNA analysis of turfgrass genetic diversity. Crop Sci. 38: 1415-1424.
Charmet G., Ravel C. and Balfourier F. 1997. Phylogenetic analysis in the Festuca /Lolium complex using molecular markers and ITS rDNA. Theor. Appl. Genet. 94: 1038-1046.
Cunico C., Cremona R. and Odoardi M. 1995. Caratterizzazione elettroforetica delle varietá di festuca iscritte al Registro Varietale. Sementi Elette 41: 17-20.
Fraser M.L., Rose-Fricker C.A. and MeyerW.A. 1999. Registration of 'Matador' tall fescue. Crop Sci. 34: 1130-1131.
Fraser M.L., Rose-Fricker C.A., Meyer W.A. and Funk C.R. 1998. Registration of 'Tar Heel' tall fescue. Crop Sci. 38: 539.
Freeman G.W., Wagg C.A. and Mileva M.M. 1996. Identification of turfgrass cultivars using reverse-phase high-performance liquid chromatography. Seed Sci. & Technol. 24: 495-504.
Gardiner S.E. and Forde M.B. 1987. SDS polyacrylamide gel electrophoresis of grass seed proteins. a method for cultivar identification of pasture grasses. Seed Sci. & Technol. 15: 663-674.
Humphreys M.W., Thomas H.M., Morgan W.G., Meredith M.R., Harper J.A., Thomas H. et al. 1995. Discriminating the ancestral progenitors of hexaploid Festuca arundinacea using genomic in situ hybridization. Heredity 75: 171-174.
Krishnan H.B. and Sleper D.A. 1997. Identification of tall fescue cultivars by sodium dodecyl sulfate polyacrylamide gel electrophoresis of seed proteins. Crop Sci. 37: 215-219.
Labombarda P., Pupilli F. and Arcioni S. 2000. Optimal population size for RFLP-assisted cultivar identification in alfalfa (Medicago sativa L.). Agronomie 20: 233-240.
Lee M. 1995. DNA markers and plant breeding programs. Adv. Agron. 55: 265-344.
Moore S.R., Devos K.M., Wang Z. and Gale M.D. 1995. Cereal genome evolution, grasses, line up and form a circle. Curr. Biol. 7: 737-739.
Nagamura Y., Antonio B.A. and Sasaki T. 1997. Rice molecular genetic map using RFLPs and its applications. Plant Mol. Biol. 35: 79-87.
Nei M. and LiW.H. 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc. Natl. Acad. Sci. USA 76: 5267-5273.
Pašakinskienė I., GriffithsC.M., BettanyA.J.E., PaplauskienėV. and Humphreys M.W. 2000. Anchored simple-sequence repeats as primers to generate species-specific DNA markers in Lolium and Festuca grasses. Theor. Appl. Genet. 100: 384-390.
Pepin G.W., McVeigh K.J. and Funk C.R. 1985. Registration of 'Houndog' tall fescue. Crop Sci. 25: 199.
Pupilli F., Businelli S., Paolocci F., Scotti C., Damiani F. and Arcioni S. 1996. Extent of RFLP variability in tetraploid populain tions of alfalfa, Medicago sativa. Plant Breed. 115: 106-112.
Pupilli F., Labombarda P., Scotti C. and Arcioni S. 2000. RFLP analysis allows for the identification of alfalfa ecotypes. Plant Breed. 119: 271-276.
Rohlf F.J. 1993. Numerical taxonomy and Multivariate Analysis System. Applied Biostatistics, Inc., New York.
Rose-Fricker C. and Meyer W.A. 1994. Registration of 'Eldorado' tall fescue. Crop Sci. 34: 1130-1131.
Rose-Fricker C., Meyer W.A. and Funk C.R. 1995. Registration of 'Safari' tall fescue. Crop Sci. 35: 1204.
Rouf Mian M.A., Andrew A.A. and Zwonitzer J.C. 2002. Determination of genetic diversity in tall fescue with AFLP markers. Crop Sci. 42: 944-950.
Saghai-Maroof M.A., Soliman K.M., Jorgensen R.A. and Allard R.W. 1984. Ribosomal DNA spacer-length polymorphisms in population dynamics. Proc. Natl. Acad. Sci. USA 81: 8014-8018.
Šiffelova G., Pavelková M., Klabochová A., Wiesner I. and Našinec V. 1997. Computer-aided RAPD fingerprinting of accessions from the ryegrass-fescue complex. J. of Agric. Sci. Cambridge 129: 257-265.
Southern E. 1975. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98: 508-517.
Stammers M., Harris J., Evans G.M., Hayward M.D. and Forster J.W. 1995. Use of PCR (RAPD) technology to analyse phylo-genetic relationships in the Lolium/Festuca complex. Heredity 74: 19-27.
Veronesi F. and Falcinelli M. 1988. Evaluation of an italian germplasm collection of Festuca arundinacea Schreb. through multivariate analysis. Euphytica 38: 211-220.
Xu W.W. and Sleper D.A. 1994a. Phylogeny of tall fescue and related species using RFLPs. Theor. Appl. Genet. 88: 685-690.
XuW.W., Sleper D.A. and Krause G.F. 1994b. Genetic diversity of tall fescue germplasm based on RFLPs. Crop Sci. 34: 246-252.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Busti, A., Caceres, M.E., Calderini, O. et al. RFLP markers for cultivar identification in tall fescue (Festuca arundinacea Schreb.). Genetic Resources and Crop Evolution 51, 443–448 (2004). https://doi.org/10.1023/B:GRES.0000023459.62679.e8
Issue Date:
DOI: https://doi.org/10.1023/B:GRES.0000023459.62679.e8