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Specific and non specific interactions involving LeX determinant quantified by lipid vesicle micromanipulation

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Abstract

Carbohydate-carbohydrate recognition is emerging today as an important type of interaction in cell adhesion. One Ca2+mediated homotypic interaction between two LewisX determinants (LeX) has been proposed to drive cell adhesion in murine embryogenesis. Here, the adhesion energies of lipid vesicles functionalised with glycolipids bearing monomeric or dimeric LeX determinants were measured in NaCl or CaCl2 media with the micropipette aspiration technique. These experiments on LeX with an environment akin to that provided by biological membrane confirmed the existence of this specific calcium dependant interaction of monomeric LeX. In contrast, dimeric LeX produced a repulsive contribution. By using a simple model involving the various contributions to the adhesion free energy, specific and non specific interactions could be separated and quantified. The involvement of calcium ions has been discussed in the monomeric and dimeric LeX lipids. Published in 2004.

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Authors
  1. Christine Gourier
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  2. Frédéric Pincet
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  3. Eric Perez
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  4. Yongmin Zhang
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  5. Jean-Maurice Mallet
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  6. Pierre Sinaÿ
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Gourier, C., Pincet, F., Perez, E. et al. Specific and non specific interactions involving LeX determinant quantified by lipid vesicle micromanipulation. Glycoconj J 21, 165–174 (2004). https://doi.org/10.1023/B:GLYC.0000044847.15797.2e

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  • DOI: https://doi.org/10.1023/B:GLYC.0000044847.15797.2e

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