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Studies of the carbohydrate-carbohydrate interaction between lactose and GM3 using Langmuir monolayers and glycolipid micelles

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Abstract

This paper describes studies of the carbohydrate-carbohydrate interaction (CCI) between micelles of a lactosyl lipid and monolayers of the glycosphingolipid GM3. The lactose Lac·GM3 interaction is involved in B16 melanoma cell adhesion and signaling processes, and a thorough understanding of the molecular details of this CCI is important for the design of new anti-adhesive and anti-metastatic agents. In this paper, we examine the influence of variations in divalent cations and subphase ionic strength on the Lac·GM3 interaction. Our results indicate that, in the absence of divalent cations, the Lac·GM3 CCI is strengthened at higher sodium chloride concentrations in the subphase. In contrast, when divalent cations are present in solution, the CCI is not as sensitive to ionic strength. These results suggest a role for both cation dependent as well as independent interactions in the Lac·GM3 CCI. Published in 2004.

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Authors
  1. Paul V. Santacroce
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  2. Amit Basu
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Santacroce, P.V., Basu, A. Studies of the carbohydrate-carbohydrate interaction between lactose and GM3 using Langmuir monolayers and glycolipid micelles. Glycoconj J 21, 89–95 (2004). https://doi.org/10.1023/B:GLYC.0000044841.12706.12

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  • DOI: https://doi.org/10.1023/B:GLYC.0000044841.12706.12

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