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Synthesis of radioactive and photoactivable ganglioside derivatives for the study of ganglioside-protein interactions

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Abstract

The procedures for the preparation of radioactive and photoactivable ganglioside derivatives have been continuously developed from 1989, when for the first time the synthesis of photoactivable tritium labeled GM1 ganglioside was presented. We described previously the synthesis of photoactivable derivatives of GM3 and GM1 gangliosides, tritium-labeled at acetyl group of sugar units, and of photoactivable GM1 and GD1b gangliosides, tritium-labeled at position 6 of the external galactose. These procedures are reviewed in detail in the present paper.

The use of these ganglioside derivatives to study the ganglioside-protein interactions and to identify proteins that specifically interact with gangliosides (including GPI-anchored proteins of the outer membrane leaflet, proteins anchored to the cytoplasmic side of the plasma membrane through a fatty acyl chain, transmembrane proteins, and soluble cytoplasmic proteins) is discussed. Published in 2004.

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Authors and Affiliations

  1. Center of Excellence on Neurodegenerative Diseases, Study Center for the Biochemistry and Biotechnology of Glycolipids, Department of Medical Chemistry, Biochemistry and Biotechnology, University of Milan, 20090, Segrate, Italy

    Laura Mauri, Simona Prioni, Nicoletta Loberto, Vanna Chigorno, Alessandro Prinetti & Sandro Sonnino

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  1. Laura Mauri
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  2. Simona Prioni
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  3. Nicoletta Loberto
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  4. Vanna Chigorno
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  5. Alessandro Prinetti
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  6. Sandro Sonnino
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Correspondence to Sandro Sonnino.

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Mauri, L., Prioni, S., Loberto, N. et al. Synthesis of radioactive and photoactivable ganglioside derivatives for the study of ganglioside-protein interactions. Glycoconj J 20, 11–23 (2003). https://doi.org/10.1023/B:GLYC.0000016738.37102.03

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  • DOI: https://doi.org/10.1023/B:GLYC.0000016738.37102.03

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