Abstract
The mitochondrial cyt b genes in the allotetraploid and triploid crucian carp as well as triploid common carp were isolated and completely sequenced. Their DNA sequences were compared with those derived from the cyt b genes of the red crucian carp, Japanese crucian carp, and common carp with MEGA 1.0 software. Phylogenetic analysis revealed the sister relationships between allotetraploid and diploid red crucian carp, between the triploid crucian carp and diploid Japanese crucian carp, and between triploid common carp and diploid common carp. Our results indicated the cyt b genes in the allotetraploid, triploid crucian carp, and triploid common carp were maternally inherited. Through maternal inheritance, the cyt b gene in the F11 tetraploid displayed extremely high similarity to that in the female parent red crucian carp after 11 generations (from F1 to F11 hybrids). Since the establishment of the new tetraploid stocks has great significance in analyzing evolutionary theory of vertebrate and in improving aquaculture industry, analysis of the cyt b gene and the elucidation of the variation of the cyt b gene DNA in different cyprinids prove that cyt b is a useful genetic marker to monitor the variations in the progeny of the crosses.
Similar content being viewed by others
References
Avise, J.C., 1991. Ten unorthodox perspectives on evolution prompted by comparative population genetic findings on mitochondrial DNA. Annu. Rev. Genet. 25: 45–69.
Bellis, C., K.J. Ashto, L. Freney, B. Blair & L.R. Griffiths, 2003. A molecular genetic approach for forensic animal species identification. Forensic Sci. Int. 134(2-3): 99–108.
Birky, Jr C.W., 1995. Uniparental inheritance of mitochondrial and chloroplast genes: mechanisms and evolution. Proc. Natl. Acad. Sci. USA 92 (25): 11331–11338.
Durand, J.D., C.S. Tsigenopoulos, E. Unlu & P. Berrebi, 2002. Phylogeny and biogeography of the family Cyprinidae in the Middle East inferred from cytochrome bDNA-evolutionary significance of this region. Mol. Phylogenet. Evol. 22(1): 91–100.
Felsenstein, J., 1985. Confidences limits on phylogenies: an approach using the bootstrap. Evolution 39: 783–791.
Guo, X.H., S.J. Liu & Y. Liu, 2003. Comparative analysis of the mitochondrial DNA control region in cyprinids with different ploidy level. Aquaculture 2003 224: 25–38.
Gyllensten, U., D. Wharton, A. Josefsson & A.C. Wilson, 1991. Paternal inheritance of mitochondrial DNA in mice. Nature 352(6332): 255–257.
Kaneda, H., J. Hayashi, S. Takahama, C. Taya, K.F. Lindahl & Yonekawa, 1995. Elimination of paternal mitochondrial DNA in intraspecific crosses during early mouse embryogenesis. Proc. Natl. Acad. Sci. USA 92(10): 4542–4546.
Kawai, K., M. Nikaido, M. Harada, S. Matsumura, L.K. Lin, Y. Wu, M. Hasegawa & N. Okada, 2003. The status of the Japanese and East Asian bats of the genus Myotis (Vespertilionidae) based onmitochondrial sequences. Mol. Phylogenet. Evol. 28(2): 297–307.
Kimura, M., 1981. Estimation of evolutionary distances between homologous nucleotide sequences. Proc. Natl. Acad. Sci. USA 78: 454–458.
Kondo, R., Y. Satta, E.T. Matsuura, H. Ishiwa, TN. Akahata & S.I. Chigusa, 1990. Incomplete maternal transmission of mitochondrial DNA in Drosophila. Genetics 126(3): 657–663.
Kotlik, P. & P. Berrebi, 2002. Genetic subdivision and biogeography of the Danubian rheophilic barb Barbus petenyiinferred from phylogenetic analysis of mitochondrial DNA variation. Mol. Phylogenet. Evol. 24(1): 10–18.
Kumar, S., K. Tamura & M. Nei, 1993. MEGA: Molecular Evolutionary Genetics Analysis, Version 1.01. Pennsylvenia State University Press, Pennsylvenia, USA.
Kvist, L., J. Martens, A.A. Nazarenko & M. Orell, 2003. Paternal leakage of mitochondrial DNA in the Great Tit (Parus major). Mol. Biol. Evol. 20(2): 243–247.
Li, S.F., S.J. Liu, X.J. Zhang, C. Luo, G.J. Zhou & Y. Liu, 2000. Study on the mtDNA from liver of allotetraploid hybrids of red crucian (?)×common carp (?). J. Fish. China 24(6): 489–493 (in Chinese with English abstract).
Li, S.F., S.J. Liu, X.J. Zhang, C. Luo, G.J. Zhou & Y. Liu, 2001. Comparative studies on mitochondrial DNA from triploid crucian carp and its parents (allotetraploid hybrids of crucian carp, ?and Carassius auratus cuvieri, ?). J. Fish. Sci. China 8(1): 1–5 (in Chinese with English abstract).
Liu, S.J., Y. Liu, G.J. Zhou, X.J. Zhang, C. Lou, H. Feng, X.X. He, G.H. Zhu & H. Yang, 2001. The formation of tetraploid stocks of red crucian carp×common carp hybrids as an effect of interspecific hybridization. Aquaculture 192: 171–186
Machordom, A. & I. Doadrio, 2001. Evolutionary history and speciation modes in the cyprinid genus Barbus. Proc. R. Soc. Lond. B Biol. Sci. 268(1473): 1297–1306.
Murakami, M., C. Matsuba & H. Fujitani, 2001. The maternal origins of the triploid ginbuna (Carassius auratus langsdorfi): phylogenetic relationships within the C. auratustaxa by partial mitochondrial D-loop sequencing. Gene. Genet. Syst. 276(1): 25–32.
Orlando, L., J.F. Mauffrey, J. Cuisin, J.L. Patton, C. Hanni & F. Catzeflis, 2003. Napoleon Bonaparte and the fate of an Amazonian rat: new data on the taxonomy of Mesomys hispidus(Rodentia: Echimyidae). Mol. Phylogenet. Evol. 27(1): 113–120.
Palumbi, S.R., 1996. Nucleic acids II: the polymerase chain reaction, in Molecular Systematics, edited by D.M. Hillis, C. Moritz & B.K. Mable. 2nd edn. Sinauer, Sunderland, MA.
Rawson, P.D., C.L. Secor & T.J. Hilbish, 1996. The effects of natural hybridization on the regulation of doubly uniparental mtDNA inheritance in blue mussels (Mytilusspp.). Genetics 144(1): 241–248.
Shitara, H., J.I. Hayashi, S. Takahama, H. Kanada & H. Yonekawa, 1998. Maternal inheritance of mouse mtDNA in interspecific hybrids: segregation of the leaked paternal mtDNA followed by the prevention of subsequent paternal leakage. Genetics 148(2): 851–857.
Sun, Y.D., S.J. Liu, C. Zhang, J.Z. Li, W.R. Huang, J. Zhang, K.K. Lou, G.J. Zhou & Y. Liu, 2003. The chromosome number and gonadal structure of F9-F11 allotetraploid crucian carp. Acta Genet. Sin. 30(5): 37–41 (in Chinese with English abstract).
Telfer, P.T., S. Souquiere, S.L. Clifford, K.A. Abernethy, M.W. Bruford, T.R. Disotell, K.N. Sterner, P. Roques, P.A. Marx & E.J. Wickings, 2003. Molecular evidence for deep phylogenetic divergence in Mandrillus sphinx. Mol. Ecol. 12(7): 2019–2024.
Thompson J.D., D.G. Higgins & T.J. Gibson. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucl. Acid. Res. 22: 4673–4680.
Tsigenopoulos, C.S., P. Rab, D. Naran & P. Berrebi, 2002. Multiple origins of polyploidy in the phylogeny of southern African barbs (Cyprinidae) as inferred from mtDNA markers. Heredity 88(6): 466–473.
Xiao, W.H., Y.P. Zhan & H.Z. Liu, 2001. Molecular systematics of xenocyprinae (Teleostei: Cyprinidae): taxonomy, biogeography, and coevolution of a special group restricted in east Asia. Mol. Biol. Evol. 18: 163–173.
Zouros, E., A. Oberhauser Ball, C. Saavedra & K.R. Freeman, 1994. An unusual type of mitochondrial DNA inheritance in the blue mussel Mytilus. Proc. Natl. Acad. Sci. USA 91(16): 7463–7467.
Rights and permissions
About this article
Cite this article
Guo, X., Liu, S., Zhang, C. et al. Comparative and Evolutionary Analysis of the Cytochrome b Sequences in Cyprinids with Different Ploidy Levels Derived from Crosses. Genetica 121, 295–301 (2004). https://doi.org/10.1023/B:GENE.0000039847.82917.c4
Issue Date:
DOI: https://doi.org/10.1023/B:GENE.0000039847.82917.c4