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The Physical Gene Hsp70 Map on Polytene Chromosomes of Anopheles darlingi from the Brazilian Amazon

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Abstract

In situ hybridization was used to determine the physical location of the Hsp70 genes in salivary polytene chromosomes of Anopheles darlingi from Manaus and Macapá, Brazil, and to assess the usefulness of the Hsp70 locus as a genetic marker in A. darlingi populations. In both populations, the double markings corresponding to the Hsp70-12A and Hsp70-14A genes were located on the right arm of chromosome 2. The Hsp70 locus was considered to be an excellent marker for studying chromosomal evolution and relationships among A. darlingi populations.

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Authors and Affiliations

  1. Coordenação de Pesquisas em Ciências da Saúde, Instituto Nacional de Pesquisas da Amazônia (INPA), Av. André Araújo, 2936, Bairro Petrópolis, 69083-000, Manaus, AM, Brasil

    Míriam Silva Rafael & Wanderli Pedro Tadei

  2. Department of Biological Sciences, Brock University, St. Catharines, Ont., Canada, L2S 3A1

    Fiona F. Hunter

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  1. Míriam Silva Rafael
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  2. Wanderli Pedro Tadei
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  3. Fiona F. Hunter
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Rafael, M.S., Tadei, W.P. & Hunter, F.F. The Physical Gene Hsp70 Map on Polytene Chromosomes of Anopheles darlingi from the Brazilian Amazon. Genetica 121, 89–94 (2004). https://doi.org/10.1023/B:GENE.0000019959.45267.d7

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  • DOI: https://doi.org/10.1023/B:GENE.0000019959.45267.d7

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