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Genetica

, Volume 121, Issue 1, pp 95–100 | Cite as

Chromosomal Mapping and Molecular Characterization of Ribosomal RNA Genes in Lebias fasciata (Teleostei, Cyprinodontidae)

  • C. Tigano
  • L. Rocco
  • V. Ferrito
  • D. Costagliola
  • A. M. Pappalardo
  • V. Stingo
Article

Abstract

Chromosome location of major (18S, 5.8S and 28S) and 5S ribosomal RNA genes (rDNAs) was examined in Lebias fasciata collected from different Italian blackish-waters, using silver (Ag)- and chromomycin A3 (CMA3)-staining and/or fluorescence in situ hybridization (FISH). Both 18S and 5S rDNA probes for FISH were obtained with polymerase chain reaction-directed cloning from genomic DNA of the examined species. Nucleolar organizer regions (NORs) containing the major rDNAs showed intraspecific polymorphism in number as detected by Ag- and CMA3-staining and FISH with the 18S rDNA probe. On the other hand, 5S rDNA loci constantly occurred on one chromosome pair and co-localized with a pair of the major rDNA loci as evidenced by two-color FISH using the 5S and 18S rDNA probes. Sequential CMA3- and Ag-NOR staining and FISH revealed apparent inactivation of some NORs. The cloned 5S rDNA was found to contain some TATA-like sequences that might play an important role in the regulation of gene expression.

AgNOR FISH Lebias fasciata ribosomal genes and teleosteans 

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Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • C. Tigano
    • 1
  • L. Rocco
    • 2
    • 2
  • V. Ferrito
    • 1
  • D. Costagliola
    • 2
  • A. M. Pappalardo
    • 1
  • V. Stingo
    • 2
  1. 1.Dipartimento di Biologia AnimaleUniversità di CataniaCatania
  2. 2.Dipartimento di Scienze della VitaSeconda Università di NapoliCasertaItaly

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