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Characterization of a New HpaI Centromeric Satellite DNA in Salmo salar

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Abstract

A highly repeated HpaI DNA family was revealed in Atlantic salmon (Salmo salar) and analyzed by Southern blotting and fluorescence in situ hybridization (FISH). In this report, we describe the nucleotide sequence, genomic structure and chromosomal localization of this HpaI repeat. This novel satellite appeared tandemly arrayed and located at centromeric areas of three acrocentric chromosome pairs as evidenced by FISH. The sequence was characterized by a high AT content (63%), a short consensus motif (A/T)(G/C)AAA(T/C) similar to other centromeric satellites motifs, and by short AT enriched stretches. The presence of this sequence in other salmonid species was also tested by Southern blot hybridization and used to analyze its evolution within this group.

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Authors and Affiliations

  1. Departamento de Genética, Facultad de Biología, Universidad de Santiago de Compostela, 15782, Santiago de Compostela

    Ana Viñas

  2. Departamento de Genética, Facultad de Veterinaria, Universidad de Santiago de Compostela, 27002, Lugo, Spain

    María Abuín, Belén G. Pardo, Paulino Martí & Laura Sánchez

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  1. Ana Viñas
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  2. María Abuín
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  3. Belén G. Pardo
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  4. Paulino Martí
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  5. Laura Sánchez
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Viñas, A., Abuín, M., Pardo, B.G. et al. Characterization of a New HpaI Centromeric Satellite DNA in Salmo salar . Genetica 121, 81–87 (2004). https://doi.org/10.1023/B:GENE.0000019927.30049.9c

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