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Primary structures and gene organizations of two types of Wap65 from the pufferfish Takifugu rubripes

Fish Physiology and Biochemistry volume 29, pages 211–224 (2003)Cite this article

Abstract

We isolated two types of cDNA clone encoding hemopexin-like protein, fWap65-1 and fWap65-2, from the pufferfish Takifugu rubripes. The deduced amino acid sequence of fWap65-1 showed 66–67% identity with those of goldfish and carp Wap65s, whereas the sequence of fWap65-2 did 44–46% identity. Both fWap65s showed 32–38% amino acid identity with mammalian hemopexins, in which fWap65-2 was more related than fWap65-1. While hemopexins contain two conserved histidine residues in their heme binding pockets, these residues were also conserved in fWap65-2, but not in fWap65-1. The exon-intron organization was highly conserved between fWap65s and human hemopexin gene, suggesting that Wap65s are fish orthologs of human hemopexin. The 5′-flanking regions of both fWap65s contained various putative transcriptional elements, including Cdx1, GATA-1, C/EBPβ and LyF-1. The expression patterns of fWap65s in various tissues of Fugu were examined by RT-PCR, demonstrating the dominant expression of both genes in liver followed by brain. In addition, the small quantities of fWap65-1 transcripts were also detected in eye, gill and gonad, whereas the transcripts of fWap65-2 could not be observed except for liver and brain. Although the average values for mRNA levels of both fWap65s in warm-acclimated fish tended to be higher than those of cold-acclimated fish, their differences were not statistically significant.

Abbreviations: DTT - dithiothreitol; LPS - lipopolysaccharide; RACE - rapid amplification of cDNA ends; SDS - sodium dodecyl sulfate; Wap65 - warm temperature acclimation-related 65 kDa protein.

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Authors and Affiliations

  1. Laboratory of Aquatic Molecular Biology and Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, 113-8657, Japan

    M. Hirayama, M. Nakaniwa, D. Ikeda & S. Watabe

  2. Nissui Oita Marine Biological Technology Center, Oita, 876-1204, Japan

    N. Hirazawa, T. Otaka, T. Mitsuboshi & K. Shirasu

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  1. M. Hirayama
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  2. M. Nakaniwa
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  3. D. Ikeda
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  4. N. Hirazawa
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  5. T. Otaka
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  6. T. Mitsuboshi
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  7. K. Shirasu
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  8. S. Watabe
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Hirayama, M., Nakaniwa, M., Ikeda, D. et al. Primary structures and gene organizations of two types of Wap65 from the pufferfish Takifugu rubripes . Fish Physiology and Biochemistry 29, 211–224 (2003). https://doi.org/10.1023/B:FISH.0000045723.52428.5e

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  • DOI: https://doi.org/10.1023/B:FISH.0000045723.52428.5e

  • cDNA cloning
  • Fugu
  • genome
  • hemopexin
  • temperature acclimation
  • transcriptional element
  • warm temperature acclimation-related 65 kDa protein

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