Abstract
Carp undergo temperature acclimation of respiratory function by altering mitochondrial ATP synthase (FoF1-ATPase) both quantitatively and qualitatively (Itoi et al. 2003). To address such acclimation temperature-dependent changes of FoF1-ATPase activity, we investigated in this study the correlation between the fatty acid composition and FoF1-ATPase activity in fast muscle of thermally acclimated carp. The quantities of saturated fatty acids of mitochondria from carp acclimated to 10 °C were significantly lower than those of carp acclimated to 30 °C. While mono- and poly-unsaturated fatty acids tended to increase with cold acclimation of carp, the molar concentration of 16:0 aldehyde in mitochondria from the 10 °C-acclimated carp were less than those from the 30 °C-acclimated fish. The specific activities of FoF1-ATPase in the 10 °C- and 30 °C-acclimated fish mitochondria were calculated to be 167±22 and 56±10 nmol/min ⋅ mg mitochondrial protein, respectively, the difference being significant at P<0.005. Taken together, the increase in FoF1-ATPase activity in fast muscle mitochondria of carp after cold temperature acclimation may be closely related to the increase of unsaturated fatty acids in mitochondria.
Abbreviations: BSA - bovine serum albumin; DHA - docosahexaenoic acid; EGTA - ethyleneglycol bis(2-aminoethylether)tetraacetic acid; EPA - eicosapentaenoic acid; FoF1-ATPase - mitochondrial ATP synthase; α-F1-ATPase - FoF1-ATPase α-subunit; β-F1-ATPase - FoF1-ATPase β-subunit; HEPES - 2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulfonic acid; SDS - sodium dodecyl sulfate; SDS-PAGE - SDS-polyacrylamide gel electrophoresis.
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Itoi, S., Kawahara, S., Kondo, H. et al. Changes in mitochondrial fatty acid composition following temperature acclimation of carp and their possible effects on FoF1-ATPase activity. Fish Physiology and Biochemistry 29, 237–244 (2003). https://doi.org/10.1023/B:FISH.0000045721.26261.a4
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DOI: https://doi.org/10.1023/B:FISH.0000045721.26261.a4
- ATP synthase
- Cyprinus carpio
- fast muscle
- mitochondria
- mitochondrial membrane
- unsaturated fatty acid