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Nutrient and amino acid profiles of egg and larvae of Asian seabass, Lates calcarifer (Bloch)

Fish Physiology and Biochemistry volume 29, pages 141–147 (2003)Cite this article

Abstract

Fertilized eggs and developing larvae of hatchery reared Asian seabass, Lates calcarifer (Bloch), were analyzed to determine the changes occurring in their proximate and amino acid (AA) composition. The fertilized dry egg weighed 31 μg and contained 13.71 μg (44%) protein, 8.48 μg (27%) lipid and 0.657 J of gross energy. Dry weight decreased by 39% during hatching. The protein, lipid and carbohydrate nutrients decreased by 4.86, 4.15 and 0.09 μg, respectively from egg to 2-days post hatching (dph) larvae (pre-feeding). The protein content of the spawned eggs and larvae were hydrolysed to AA in the laboratory. The fertilized eggs had a total AA content of 42% of their dry weight. The egg contained 1.287 μg, 1.132 μg, 0.964 μg, 0.942 μg, 0.787 μg and 0.713 μg of leucine, lysine, arginine, valine, threonine and phenylalanine, respectively and these six indispensable amino acids (IAA) constituted approximately 78% of the total IAA. In the early feeding stages of L. calcarifer larvae, the ratio of IAA/DAA increased from 0.797 in the pre-feeding stage to 1.632 after 2 days of feeding. During larval growth of L. calcarifer, the percentage contribution of isoleucine and leucine to total IAA contents increased, while it decreased for lysine, phenyl alanine and arginine. L. calcarifer larvae were found to have proteins, which are rich in glutamic acid, leucine and lysine, and poor in threonine and histidine, suggesting high dietary leucine and lysine IAA requirement.

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References

  • Amend, D.F. and Fender, D.C. 1976. Uptake of bovine serum albumin by rainbow trout from hyperosmotic solutions: A model for vaccinating fish. Science 192: 793–794.

    PubMed  CAS  Google Scholar 

  • Applebaum, S.L. and Rønnestad, I. 2003. Absorption, assimilation and catabolism of individual free amino acids by larval Atlantic halibut (Hippoglossus hippoglossus). Aquaculture 230: 313–322.

    Article  CAS  Google Scholar 

  • Bromage, N.R. 1995. Broodstock management and seed quality-general considerations. In: Broodstock Management and Egg and Larval quality. pp. 1–24. Edited by N.R. Bromage and R.J. Roberts. Blackwell Science, London.

    Google Scholar 

  • Buddington, R.K. 1992. Intestinal nutrient transport during ontogeny of vertebrates. Am. J. Physiol. 262: R503–R509.

    Google Scholar 

  • Buddington, R.K. and Diamond, J.M. 1989. Ontogenic development of intestinal transports. Am. Rev. Physiol. 51: 601–619.

    Article  CAS  Google Scholar 

  • Cetta, C.M. and Capuzzo, J.M. 1982. Physiological and biochemical aspects of embryonic and larval development of the winter flounder, Pseudopleuronectes americanus. Mar. Biol. 71: 327–337.

    Article  Google Scholar 

  • Conceição, L.E.C., Grasdalen, H. and Rønnestad, I. 2003. Amino acid requirements of fish larvae and post larvae: new tools and recent findings. Aquaculture 227: 221–232.

    Article  CAS  Google Scholar 

  • Conceição, L.E.C., Ozorio, R.O.A., Suurd, E.A. and Verreth, J.A.J. 1998. Amino acid profiles and amino acid utilization in larval catfish (Claria gariepinus): effects of ontogeny and temperature. Fish Physiol. Biochem. 19: 43–57.

    Article  Google Scholar 

  • Conceição, L.E.C., Vander Meeren, T., Verreth, J.A.J., Evjen, M.S., Houlihan, D.F. and Fyhn, H.J. 1997. Amino acid metabolism and protein turn over in larval turbot (Scophthalmus maximus) from first feeding until metamorphosis, fed natural zooplankton or Artemia.Mar. Biol. 129: 255–265.

    Article  Google Scholar 

  • Cowey, C.B. and Sargent, J.R. 1979. Nutrition. In: Fish Physiology. Vol. III pp. 1–70. Edited by W. S. Hoar and D.J. Randall. Academic Press, New York.

    Google Scholar 

  • Dubios, M., Gillies, K.A., Hamilton, J.K., Reberts, P.A. and Smith, F. 1956. Colorimetric method for determination of sugars and related substances. Anal. Chem. 28: 350–356.

    Article  Google Scholar 

  • Finlayson, A.J. 1964. Amino acid recovering in the analysis of some feed samples. Can. J. Plant Sci. 45: 184–188.

    Article  Google Scholar 

  • Finn, R.N. 1994. Physiological Energetics of Developing Marine Fish Embryos and Larvae. Dr. Scient. Thesis, University of Bergen, Norway.

    Google Scholar 

  • Finn, R.N., Fyhn, H.J. and Evjen, M.S. 1995. Physiological energetics of developing embryos and yolk-sac larvae of Atlantic cod (Gadus morhua). I. Respiration and nitrogen metabolism. Mar. Biol. 124: 355–369.

    Article  CAS  Google Scholar 

  • Folch, J., Lees, H. and Sloane-stanley, G.H. 1957. A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 726: 497–509.

    Google Scholar 

  • Fyhn, H.J. 1989. First feeding of marine fish larvae. Are free amino acids the source of energy? Aquaculture 88: 111–120.

    Article  Google Scholar 

  • Fyhn, H.J. 1990. Energy production in marine fish larvae with emphasis on free amino acids as a potential fuel. In: Nutrition in wild and domestic animals Vol. 5. pp. 176–192. Edited by J. Mellinger. Karger, Basel.

  • Gomez, K.A. and Gomez, A.A. 1984. Statistical procedures for agricultural research 2nd Edition pp. 8–19. John Wiley and Sons, New York.

  • Greenwood, P.H. 1976. A review of the family centropomidae (Pisces Perciformes). Bull. Br. Mus. Nat. Hist. (Zoology). 29: 1–81.

    Google Scholar 

  • Heming, T.A. and Buddington, R.K. 1988. Yolk absorption in embryonic and larval fishes. In: Fish Physiology. Vol XI. part A pp. 407–446. Edited by W.S. Hoar, D.J. Randall and J.R. Brett. Academic Press, New York.

    Google Scholar 

  • Ketola, G.H. 1982. Amino acid nutrition of fishes: requirement and supplementation of diets. Comp. Biochem. Physiol. 73B: 17–24.

    CAS  Google Scholar 

  • Kim, K., Grimshaw, T.W., Kayes, T.B. and Amudson, C.H. 1992. Effect of fasting or feeding diets containing different levels of protein or amino acids on the activities of the liver amino acid-degrading enzymes and amino acid oxidation in rainbow trout (Oncorhynchus mykiss). Aquaculture 107: 89–105.

    Article  CAS  Google Scholar 

  • Kjørsvik, E., Mangor-jensen, A. and Holmefjord, I. 1989. Egg quality in marine fishes. Ny Fisk. Rapp. L. Nr. 5/89, Norway.

  • Kohno, H., Hara, S. and Taki, Y. 1986. Early larval development of the seabass Lates calcariferwith emphasis on the transition of energy sources. Bull. Jap. Soc. Sci. Fish. 52: 1719–1725.

    Google Scholar 

  • Lin, M. and Arnold, C. 1982. Transfer of glucose from seawater to the blood of red fish. Proc. 66th Ann. Meet. Fed. Am. Soc. Exp. Biol. 41: 489.

    Google Scholar 

  • Lowry, O.H., Rosenbrough, N.J., Farr, A.L. and Randall, R.J. 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193: 265–275.

    PubMed  CAS  Google Scholar 

  • Marte, C.L. 2003. Larviculture of marine species in Southeast Asia: current research and industry prospects. Aquaculture 227: 293–304.

    Article  Google Scholar 

  • Nakagawa, H. 1970. Studies on rainbow trout egg Salmo gairdneri irideus. II. Carbohydrate in the egg protein. J. Fac. Fish. Anim. Husb. Hiroshima Univ. 9: 57–63.

    CAS  Google Scholar 

  • Peihong Zhu, Christopher, C.P. and Brown, J.A. 2003. Lipid and amino acid metabolism during early development of Atlantic halibut (Hippoglossus hippoglossus). Aquacult. Int. 11: 43–52.

    Article  Google Scholar 

  • Rainuzzo, J.R. 1993. Lipid in Early stages of Marine Fish. Dr. Scient. Thesis, University of Trondheim, Norway.

    Google Scholar 

  • Rønnestad, I. and Fyhn, H.J. 1993. Metabolic aspects of free amino acids in developing marine fish eggs and larvae. Rev. Fish. Sci. 1: 239–259.

    Article  Google Scholar 

  • Rønnestad, I. and Naas, K.E. 1993. Routine metabolism in Atlantic halibut at first feeding-a first step towards an energetic model. In: Physiology and Biochemistry of marine fish larval development. pp. 279–284. Edited by B.T. Walther and H.J. Fyhn. University of Bergen, Norway.

    Google Scholar 

  • Rønnestad, I., Finn, R.N., Groot, E.P. and Fyhn, H.J. 1992. Utilization of free amino acids related to energy metabolism of developing eggs and larvae of lemon sole, Microstomus kitt, reared in the laboratory. Mar. Ecol. Prog. Ser. 88: 195–205.

  • Rønnestad, I., Koven, W.M., Tandler, A. and Harel, M. 1994. Energy metabolism during development of eggs and larvae of gilt head sea bream (Sparus aurata). Mar. Biol. 120: 187–196.

    Article  Google Scholar 

  • Rønnestad, I., Robertson, R.R. and Fyhn, H.J. 1996. Free amino acids and protein content in pelagic and demersal eggs of tropical marine fishes. In: The Fish Egg. pp. 81–84. Edited by MacKinlay, D.D. and Eldridge, M. American Fisheries Society, Physiology Section. Bethesda.

    Google Scholar 

  • Rønnestad, I., Thorsen, A. and Finn, R.N. 1999. Fish larval nutrition: a review of recent advances in the roles of amino acids. Aquaculture 177: 201–216.

    Article  Google Scholar 

  • Rønnestad, I., Tonheim, S.K., Fyhn, H.J., Rojas-Garcia, C.R., Kamisaka, Y., Koven, W., Finn, R.N., Terjesen, B.F., Barr, Y. and Conceição, L.E.C. 2003. The supply of amino acids during early feeding stages of marine fish larvae: a review of recent findings. Aquaculture 227: 147–164.

    Article  CAS  Google Scholar 

  • Rosenthal, H. and Alderdice, D.F. 1976. Sub lethal effects of environmental stressors, natural and pollutional on marine fish eggs and larvae. J. Fish. Res. Board Can. 33: 2047–2065.

    CAS  Google Scholar 

  • Rust, M.B., Hardy, R.W. and Stickney, R.R. 1993. A new method for force-feeding larval fish. Aquaculture 116: 341–352.

    Article  Google Scholar 

  • Sargent, J.R. 1995. Origin and functions of egg lipids: nutritional implications. In: Broodstock management and Egg and Larval Quality. pp. 353–372. Edited by N.R. Bromage and R.J. Roberts. Blackwell Science, London.

    Google Scholar 

  • Segner, H. and Verreth, J. 1995. Metabolic enzyme activities in larvae of the African catfish, Clarias gariepinus: changes in relation to age and nutrition. Fish Physiol. Biochem. 14: 385–398.

    Article  CAS  Google Scholar 

  • Sivaloganathan, A., Walford, J., Ip, Y.K. and Lam, T.J. 1998. Free amino acids and energy metabolism in eggs and larvae of seabass, Lates calcarifer. Mar. Biol. 131: 695–702.

    Article  CAS  Google Scholar 

  • Southgate, P.C., Lee, P.S. and Rimmer, M.A. 1994. Growth and biochemical composition of cultured seabass (Lates calcarifer) larvae. Asian Fish. Sci. 7: 241–247.

    Google Scholar 

  • Spackman, D.H., Stein, W.H. and Moore, S. 1958. Automatic recording apparatus for use in the chromatography of amino acids. Anal. Chem. 30: 1190–1195.

    Article  CAS  Google Scholar 

  • Tamaru, C. S., Harry, A. and Lee, C.S. 1992. Fatty acid and amino acid profiles of spawned eggs of striped mullet, Mugil cephalusL. Aquaculture 105: 83–94.

    Article  CAS  Google Scholar 

  • Terner, C. 1979. Metabolism and energy conversion during early development. In: Fish Physiology. Vol III pp. 261–268. Edited by W.S. Hoar and D.J. Randall. Academic Press, New York.

    Google Scholar 

  • Thirunavukkarasu, A.R., Kailasam, M., Kishore Chandra, P., Shiranee, P., Mathew Abraham, Charles, A.V.K. and Subburaj, R. 2001. Captive broodstock development and breeding of Seabass, Lates calcarifer(Bloch) in India. In: Perspectives in Mariculture. pp. 111–124. Edited by N.G. Menon and P.P. Pillai. The Marine Biological Association of India. Cochin.

    Google Scholar 

  • Thorsen, A., Fyhn, H.J. and Wallace, R. 1993. Free amino acids as osmotic effectors for oocyte hydration in marine fishes. In: Physiology and Biochemistry of Fish Larval Development. pp. 94–98. Edited by B.T. Walther and H.J. Fyhn. University of Bergen, Bergen.

    Google Scholar 

  • Tiensongrusmee, B., Chantarasri, S., Budileksonos, S., Yowono, S.K. and Santoso, H. 1989. Propagation of seabass, Lates calcarifer, in captivity In: Sea farming Development Project. pp. 1–55. INS/81/008. UNDP, FAO. Jakarta, Indonesia.

    Google Scholar 

  • Tucker, J.W., Russell, D.J. and Rimmer, M.A. 2002. Barramundi culture: a success story for aquaculture in Asia and Australia. World Aquacult. 33: 53–59.

    Google Scholar 

  • Vazquez, R., Gonzalez, S., Rodriguez, A. and Mourente, G. 1994. Biochemical composition and fatty acid content of fertilized eggs, yolk sac stage larvae of Senegal sole, (Solea senegalensisKaup). Aquaculture 119: 273–286.

    Article  CAS  Google Scholar 

  • Vetter, R.D., Houdson, R.E. and Arnold, C. 1983. Energy metabolism in a rapidly developing marine fish egg, the Red drum, Scianops ocellata.Can. J. Fish. Aquat. Sciences. 40: 627–634.

    CAS  Google Scholar 

  • Walford, J. and Lam, T.J. 1993. Development of digestive tract and proteolytic enzyme activity in seabass (Lates calcarifer). Aquaculture 109: 187–205.

    Article  CAS  Google Scholar 

  • Watanabe, T. and Kiron, V. 1994. Review-Prospects in larval fish dietetics. Aquaculture 124: 223–251.

    Article  Google Scholar 

  • Wiggins, T.A., Bender, T.R., Mudrack, V.A. and Coll, J.A. 1985. The development, feeding, growth and survival of cultured American shad larvae through the transition from endogenous to exogenous nutrition. Prog. Fish-Cult. 47: 87–93.

    Article  Google Scholar 

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Authors and Affiliations

  1. Central Institute of Brackishwater Aquaculture (Indian Council of Agricultural Research) 75, Santhome High Road, Raja Annamalai Puram Chennai, 600 028, India

    J. Syama Dayal, S. Ahamad Ali, A.R. Thirunavukkarasu, M. Kailasam & R. Subburaj

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  1. J. Syama Dayal
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  2. S. Ahamad Ali
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  3. A.R. Thirunavukkarasu
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Dayal, J.S., Ahamad Ali, S., Thirunavukkarasu, A. et al. Nutrient and amino acid profiles of egg and larvae of Asian seabass, Lates calcarifer (Bloch). Fish Physiology and Biochemistry 29, 141–147 (2003). https://doi.org/10.1023/B:FISH.0000035936.93304.c2

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  • DOI: https://doi.org/10.1023/B:FISH.0000035936.93304.c2

  • amino acids
  • biochemical composition
  • fish egg and larvae
  • larval nutrition

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