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Molecular cloning of Insulin-like Growth Factor-I from triangular bream (Megalobrama terminalis)

Fish Physiology and Biochemistry volume 29, pages 91–95 (2003)Cite this article

Abstract

Triangular bream (Megalobrama terminalis) IGF-I DNA and gene were cloned from triangular bream liver for the first time by RT-PCR. Sequence analysis indicated that the IGF-I cDNA consisted of 486 nucleotides encoding 117 amino acids which spanned the complete signal peptide, B, C, A, D and E domains. Analysis of the E domain indicated that triangular bream IGF-I belongs to the IGF-I Ea-2 subtype. Compared to bluntnose bream (Megalobrama amblycephala), another member of the same Megalobrama genus, triangular bream IGF-I shared 99.8% identity in cDNA sequence and 99.4% in predicted amino acid sequence. However, considerable differences were found in comparison to with common carp (Cyprinus carpio) and grass carp (Ctenopharyngodon idellus), which are members of the same family but of a different genus.

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References

  • Bai, J.J., Lao, H.H., Ye, X., et al. 2001. Molecular cloning and sequence analysis of insulin-like growth factor I cDNA from bluntnose bream. Zoological Research. 22(6): 502–506.

    CAS  Google Scholar 

  • Cao, Q.P., Duguay, S.T., Plisetskaya, E.M. et al. 1989. Nucleotide sequence and growth hormone-regulated expression of salmon insulin-like growth factor I mRNA. J. Mol. Endcrinol. 3: 2005–2010.

    CAS  Google Scholar 

  • Duan C. 1997. The insulin-like growth factor system and its biological actions in fish. J. Amer Zool. 37: 491–503.

    CAS  Google Scholar 

  • Duguay, S.J., Zhang, L.J., Steiner, D.F., Funkenstein, B., et al. 1996. Development and tissue regulated expression of insulinlike factor I and II mRNA in Sparus aurata. J. Mol. Endocrinol. 16: 123–132.

    PubMed  CAS  Google Scholar 

  • Hua, Y.M., Lin, H.R. 2001. Cloning and expression in prokaryote of grass carp IGF-I cDNA. Acta Zoologica Sinica. 47(3): 274–279.

    CAS  Google Scholar 

  • Kermouni, A., Mahmoud, SS., Wang, S., Moloney, M., Habibi, HR. 1998. Cloning of a full-length insulin-like growth factor-I complementary DNA in the goldfish liver and ovary and development of a quantitative PCR method for its measurement. J. General & comparative Endocrinology. 111(1): 51–60.

    Article  CAS  Google Scholar 

  • Liang, Y.H., Cheng, C.H., Chan, K.M. 1996. Insulin-like growth factor-I Ea-2 is the predominantly expressed form of IGF in common carp (Cyprinus oarpio). J. Mol. Marine Biol. Biotechnol. 5 (2): 145–152.

    CAS  Google Scholar 

  • Loffing-Cueni, D., Schmid, A.C., Graf, H., et al. 1998. IGF-I in the bony fish Cottus scorpius: cDNA expression and differential localization in brain and islets. J. Mol. Cell. Endocrinol. 141(1-2): 187–194.

    Article  CAS  Google Scholar 

  • Li, S.F., Zhu, Z.W., Zou, S.M., et al. 2002. Interspecific phylogenies and intraspecific genetic differences of genus Megalobrama: bluntnose black bream (M. amblycephala), Guangdong black bream (M. Hoffmanni) and black bream (M. Terminalis). Acta Zoologica Sinica. 48(3): 339–345.

    CAS  Google Scholar 

  • Mcrory, J.E., Sherwood, N.M. 1994. Catfish express two forms of insulin-like growth factor-I (IGF-I) in the brain, Ubiquitous IGFI and brain-specific IGF-I. J. Biol. Chem. 269(28): 18588–18592.

    PubMed  CAS  Google Scholar 

  • Reinecke, M., Collet, C. 1998. The phylogeny of the insulin-like growth factors. International Review of Cytology. 183: 1–94.

    Article  PubMed  CAS  Google Scholar 

  • Reinecke, M., Schmid, A., Ermatinger, R., Loffing-Cueni, D. 1997. Insulin-like growth factor I in the teleost oreochromis mossambicus, the tilapia: gene sequence, tissue expression, and cellular localization. J. Endocrinology. 138(9): 3613–3619.

    Article  CAS  Google Scholar 

  • Rinderknecht, E., Humbel, R.E. 1987. The amino acid sequence of human insulin-like growth factor I and its structure homology with proinsulin J. Biol. Chem. 8: 2769–2776.

    Google Scholar 

  • Schmid, A.C., Nef, E., Kloas, W., et al. 1999. Insulin-like growth factor-I + II in the ovary of the bony fish Oreochromis mossambicus: Localization, expression, cDNA sequence. J. Mol. Cell. Endocrinl. 156: 141–149.

    Article  CAS  Google Scholar 

  • Shamblott, M.J., Chen, T.T. 1993. Age-related and tissue-specific levels of insulin-like growth factor mRNA in a teleost. J. Mol. Mar. Biotechnol. 2: 351–361

    CAS  Google Scholar 

  • Shimatsu, A., Rotwein, P. 1987. Mosaic evolution of the insulin-like growth factors. J. Biol. Chem. 262: 7894–7900.

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. College of Animal Sciences, Zhejiang University, Hangzhou, Zhejiang, 310029, P.R

    Fudan Tong, Hongyun Liu & Qun Wang

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  1. Fudan Tong
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  2. Hongyun Liu
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  3. Qun Wang
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Correspondence to Fudan Tong.

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Tong, F., Liu, H. & Wang, Q. Molecular cloning of Insulin-like Growth Factor-I from triangular bream (Megalobrama terminalis). Fish Physiology and Biochemistry 29, 91–95 (2003). https://doi.org/10.1023/B:FISH.0000035889.36190.f7

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  • DOI: https://doi.org/10.1023/B:FISH.0000035889.36190.f7

  • carps
  • IGF-I
  • species differences
  • triangular bream (Megalobrama terminalis)

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