Abstract
To identify the down-stream gene products of estrogen during ovarian differentiation, we performed subtractive hybridization using mRNA derived from normal and estrogen treated XY gonads. The clones obtained after subtractive hybridization screening were re-screened by reverse-Northern analysis. Finally seven up-regulated and three down-regulated gene products were obtained.
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References
Kobayashi, T., Kajiura-Kobayashi, H. and Nagahama, Y. 2000. Differential expression of vasa homologue gene in the germ cells during oogenesis and spermatogenesis in a teleost fish, tilapia, Oreochromis niloticus. Mech. Dev. 99: 139–142.
Nakamura, M., Kobayashi, T., Chang X-T. and Nagahama, Y. 1998. Gonadal sex differentiation in teleost fish. J. Exp. Zool. 281: 362–372.
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Kajiura-Kobayashi, H., Kobayashi, T. & Nagahama, Y. Estrogen-induced and suppressed gene expression during XY sex reversal in a teleost fish, tilapia. Fish Physiology and Biochemistry 28, 153 (2003). https://doi.org/10.1023/B:FISH.0000030507.80335.4e
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DOI: https://doi.org/10.1023/B:FISH.0000030507.80335.4e
- aromatase
- estrogen
- sex differentiation
- sex reversal
- tilapia