Abstract
The sandwich cell immunoblot assay confirmed that salmon LH-cells secreted the free form α-subunit along with the dimeric LH, but lower in amounts than the LH.
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References
Naito, N., Hyodo, S., Okumoto, N. and Nakai, Y. 1991. Differential production and regulation of gonadotropin (GTH I and GTH II) in the pituitary gland of rainbow trout, Oncorhynchus mykiss, during ovarian development. Cell Tiss. Res. 266: 457–467.
Naito, N., Koide, Y., Amano, M., Ikuta, K., Kawauchi, H., Aida, K., Kitamura, S. and Nakai, Y. 1995. The biased intracellular accumulation of the β-subunit of salmon gonadotropin (GTH II) in the pituitary of rainbow trout Oncorhynchus mykiss, during gametogenesis. Cell Tiss. Res. 279: 93–99.
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Naito, N., Koide, Y., Yada, T. et al. Salmon gonadotropes secrete free form alpha (α)-subunit: quantitative analysis by the sandwich cell immunoblot assay. Fish Physiology and Biochemistry 28, 103–108 (2003). https://doi.org/10.1023/B:FISH.0000030490.89912.f6
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DOI: https://doi.org/10.1023/B:FISH.0000030490.89912.f6
- gonadotropin
- α-subunit
- free form α-subunit
- cell immunoblot assay
- immunocytochemistry
- Oncorhynchus mykiss