Abstract
cDNA clones encoding gonadotropin (GTH) α, follicle-stimulating hormone (FSH) β and luteinzing hormone (LH) β were isolated from the pituitaries of maturing Manchurian trout (Brachymystax lenok tsinlingensis) and sequenced. The deduced amino acid sequences of GTH subunits showed high identities to masu salmon, Oncorhynchus masou: GTHα1 (95%), FSHβ (92%) and LHβ (97%), respectively. We are also attempting to produce recombinant FSH and LH using a eukaryotic expression system. In a pilot experiment, LH was secreted to the culture medium at 48 and 60 hrs after transfection. The results will be helpful to develop controlled reproduction of exterminating Manchurian trout.
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References
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Choi, E., Jin, D. & Sohn, Y. Gonadotropins in the Manchurian trout, Brachymystax lenok tsinlingensis . Fish Physiology and Biochemistry 28, 89–90 (2003). https://doi.org/10.1023/B:FISH.0000030483.11026.e5
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DOI: https://doi.org/10.1023/B:FISH.0000030483.11026.e5
Keywords
- Amino Acid Sequence
- Expression System
- cDNA Clone
- Deduce Amino Acid Sequence
- High Identity