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Characterization of the inhibitory dopamine receptor from the pituitary of tilapia

Fish Physiology and Biochemistry volume 28, pages 73–75 (2003)Cite this article

Abstract

The study was aimed to characterize the inhibitory dopamine (DA) receptor in the pituitary of tilapia. DA D2 and D3 agonists inhibited GnRH-stimulated LH release from tilapia pituitary cells more efficiently than D4 agonists. However, no significant differences were observed between the efficiencies of D2 and D3 drugs. In order to elucidate the signal transduction of the tilapia DA receptor we performed transfection of COS-7 cells with tilapia D2 DA receptor that resulted in the inhibition of forskolin-stimulated cAMP accumulation. Moreover, the tilapia D2 dopamine receptor inhibits adenylate cyclase activity via coupling to pertussis toxin-sensitive G proteins of the Gi/Go family. Our results also show that estradiol up-regulates taDA-R mRNA.

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References

  • Ben-Jonathan, N. and Hnasko, R. 2001. Dopamine as a prolactin (PRL) inhibitor. Endocr Rev. 22: 724–63.

    Google Scholar 

  • Levavi-Sivan, B., Ofir, M. and Yaron, Z. 1995. Possible Sites of Dopaminergic Inhibition of Gonadotropin-Release from the Pituitary of a Teleost Fish, Tilapia. Molecular and Cellular Endocrinology 109: 87–95.

    Google Scholar 

  • Levavi-Sivan, B., Park, B.H., Fuchs, S. and Fishburn, C.S. 1998. Human D3 dopamine receptor in the medulloblastoma TE671 cell line: cross-talk between D1 and D3 receptors. FEBS Lett 439: 138–42.

    Google Scholar 

  • Obadiah, J., Avidor-Reiss, T., Fishburn, C.S., Carmon, S., Bayewitch, M., Vogel, Z., Fuchs, S. and Levavi-Sivan, B. 1999.Adenylyl cyclase interaction with the D2 dopamine receptor family; differential coupling to Gi, Gz, and Gs. Cell Mol. Neurobiol. 19: 653–64.

    Google Scholar 

  • Rosenfeld, H., Levavi-Sivan, B., Gur, G., Melamed, P., Meiri, I., Yaron, Z. and Elizur, A. 2001. Characterization of tilapia FSH beta gene and analysis of its 5_ flanking region. Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology 129: 389–398.

    Google Scholar 

  • Vacher, C., Ferriere, F., Marmignon, M.H., Pellegrini, E. and Saligaut, C. 2002. Dopamine D2 receptors and secretion of FSH and LH: role of sexual steroids on the pituitary of the female rainbow trout. General and Comparative Endocrinology 127: 198–206.

    Google Scholar 

  • Yaron, Z., Gur, G., Melamed, P., Rosenfeld, H., Levavi-Sivan, B. and Elizur, A. 2001 Regulation of gonadotropin subunit genes in tilapia. Comparative Biochemistry and Physiology BBiochemistry & Molecular Biology 129: 489–502.

    Google Scholar 

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Authors and Affiliations

  1. Department of Animal Sciences, Faculty of Agriculture, Food and Environmental Quality Sciences, The Hebrew University, Israel

    Levavi-Sivan Berta, Avitan Ayelet & Kanias Tamir

Authors
  1. Levavi-Sivan Berta
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  2. Avitan Ayelet
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  3. Kanias Tamir
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Berta, LS., Ayelet, A. & Tamir, K. Characterization of the inhibitory dopamine receptor from the pituitary of tilapia. Fish Physiology and Biochemistry 28, 73–75 (2003). https://doi.org/10.1023/B:FISH.0000030479.47055.24

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  • DOI: https://doi.org/10.1023/B:FISH.0000030479.47055.24

Keywords

  • Estradiol
  • Pertussis
  • Dopamine Receptor
  • Adenylate Cyclase
  • Pituitary Cell

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