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Assessment of genetic diversity in selected breeding lines and cultivars of canola quality Brassica juncea and their implications for canola breeding

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Abstract

AFLP markers were used to assess the genetic diversity of 77 breeding lines from three of the world's major canola qualityBrassica juncea breeding programs from Canada (Agriculture and Agri-Food Canada and Saskatchewan Wheat Pool) and Australia (Agriculture Victoria). The objectives of the paper were to assess the genetic diversity within and between these three breeding programs and to assess genetic diversity of the canola quality germplasm as compared to mustard quality B. juncea. Fifteen lines of mustard quality B. juncea from India, China, Russia and Australia were also included in the investigation. Ten EcoR1/Mse1 based primer pairs generated 751 scorable fragments with an average of 26 polymorphic bands per primer pair (35%). Similarity coefficients were calculated using the Simple Matching coefficient and adendrogram was developed using the UPGMA procedure, resulting in germplasm being partitioned into five main groups. Line specific markers were discovered that have potential in enhancing the efficiency of individual breeding programs using breeding techniques like accelerated backcrossing. Further understanding the genetic diversity within and between programs has implications for future breeding and collaboration within and between the three programs.

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Authors and Affiliations

  1. Department of Primary Industries, Victorian Institute for Dryland Agriculture, Private Bag 260, Horsham Vic, 3401, Australia

    W.A. Burton & P.A. Salisbury

  2. Agriculture and Agri-Food Canada, 107 Science Place, Saskatoon, Saskatchewan, Canada, S7N 0X2

    V.L. Ripley

  3. Saskatchewan Wheat Pool, Research and Development, 201-407 Downey Road, Saskatoon, Saskatchewan, Canada, S7N 4L8

    D.A. Potts

  4. Institute of Land and Food Resources, University of Melbourne, Victoria, 3010

    P.A. Salisbury

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  1. W.A. Burton
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  2. V.L. Ripley
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  3. D.A. Potts
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  4. P.A. Salisbury
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Burton, W., Ripley, V., Potts, D. et al. Assessment of genetic diversity in selected breeding lines and cultivars of canola quality Brassica juncea and their implications for canola breeding. Euphytica 136, 181–192 (2004). https://doi.org/10.1023/B:EUPH.0000030668.62653.0d

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  • DOI: https://doi.org/10.1023/B:EUPH.0000030668.62653.0d

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