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Diversity among melon landraces (Cucumis melo L.) from Greece and their genetic relationships with other melon germplasm of diverse origins

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Abstract

Diversity among 17 melon landraces and inbred lines of Group Cantalupensis, Inodorus, and Flexuosus germplasm from Greece was assessed using 24 RAPD primers, 11 morphological traits of fruit, two yield-related characteristics, and resistance to powdery mildew. Accessions were genetically diverse and the greatest variation was detected in Group Flexuosus. Comparative analysis of Greek germplasm and an array of previously characterized reference accessions [RA; 68 from Africa (15), United States (U.S.; 5), Central Europe (14), Japan (19), and Spain (15)], spanning major market classes employing 19 RAPD primers (standard marker array), indicates that all but one of the accessions from Greece showed genetic affinities among themselves and with 23 RA of various origin (i.e., Japan, U.S., Central Europe, Spain and Israel) and market class. Accessions from Greece were, however, distinct from the African landraces and most Spanish melons. Greek accessions also showed little genetic affinity with the Casabas (Blanco and Crenshaw), Ogen, and U.S. Eastern Market accessions. Group Flexuosus and Inodorus accessions from Greece possessed genetic affinities with Western Asian (Group Conomon) and Mediterranean (Group Inodorus) market classes, respectively. The uniqueness of Greek melon landraces portends their potential usefulness for the enhancement of U.S., European, and Asian melon market classes.

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References

  • Akashi, Y., N. Fukuda, T. Wako, M. Masuda & K. Kato, 2002. Genetic variation and phylogenetic relationships in East and South Asian melons, Cucumis melo L., based on the analysis of five isozymes. Euphytica 125: 385–396.

    Article  CAS  Google Scholar 

  • Esquinas-Alcázar, J.T., 1977. Alloenzyme Variation and Relationships in the Genus Cucumis. PhD Dissertation University of California, Davis, 170 pp.

    Google Scholar 

  • Fanourakis, N., Z. Tsekoura & E. Nanou, 2000. Morphologicalcharacteristics and powdery mildew resistance of Cucumis melo land races in Greece., In: N. Katzir & H.S. Paris (Eds.), Proc Cucurbitaceae 2000. Acta Hort 510: 241–245.

  • García, E., M. Jamilena, J.I. Álvarez, T. Arnedo, J.L. Oliver & R. Lozano, 1998. Genetic relationships among melon breeding lines revealed by RAPD markers and agronomic traits. Theor Appl Genet 96: 878–885.

    Article  Google Scholar 

  • Horejsi, T. & J.E. Staub, 1999. Genetic variation in cucumber (Cucumis sativus L.) as assesssed by Random Amplified Polymorphic DNA. Gen Res Crop Evol 46: 337–350.

    Article  Google Scholar 

  • Jaccard, P., 1908. Nouvelles reserches sur la distribution florale. Bull Soc Vaud Sci Nat 44: 223–270.

    Google Scholar 

  • Katzir, N., T. Danin-Poleg, G. Tzuri, Z. Karchi, U. Lavi & P.B. Cregan, 1996. Length polymorphism and homologies of microsatellites in several Cucurbitaceae species. Theor Appl Genet 93: 1282–1290.

    Article  CAS  Google Scholar 

  • Kirkbride, J.H., Jr., 1993. Biosystematics Monograph of the Genus Cucumis (Cucurbitaceae). Parkway Publishers, Boone, NC.

    Google Scholar 

  • López Sesé, A.I., J.E. Staub, N. Katzir & M.L. Gómez-Guillamón, 2002. Estimation of between and within accession variation in selected Spanish melon germplasm using RAPD and SSR markers to assess strategies for large collection evaluation. Euphytica 127: 41–51.

    Article  Google Scholar 

  • López-Sesé, A., J.E. Staub & M.L. Gomez-Guillamon, 2003. Genetic analysis of Spanish melon (Cucumis melo L.) germplasm using a standardized molecular marker array and reference accessions. Theor. Appl. Genet. 108: 41–52.

    Article  PubMed  Google Scholar 

  • Maniatis, T., E.F. Fritsch & J. Sambrook, 1982. Molecular Cloning: A Laboratory Manual. Cold Spring Harbour Laboratory Press, Cold Spring Harbour, New York.

    Google Scholar 

  • McCreight, J.D., H. Nerson & R. Grumet, 1993. Melon, Cucumis melo L. In: G. Kallos & B.O. Bergh (Eds.), Genetic Improvement of Vegetable Crops. pp. 267–294. Pergamon Press, New York.

    Google Scholar 

  • Mliki, A., J.E. Staub, S. Zhangyong & A. Ghorbel, 2001. Genetic diversity in melon (Cucumis melo L.): An evaluation of African germplasm. Gen Res Crop Evol 48: 587–597.

    Google Scholar 

  • Monforte, A.J., J. Garcia-Mas & P. Arus, 2003. Genetic variability in melon based on microsatellite variation. Plant Breed 122: 153–157.

    Article  Google Scholar 

  • Munger, H.M. & R.W. Robinson, 1991. Nomenclature of Cucumis melo L. Cucurbit Genet Coop Rep 14: 43–44.

    Google Scholar 

  • Nakata, E., J.E. Staub, A. López-Sesé & N. Katzir. 2004. Genetic diversity in Japanese melon (Cucumis melo L.) as assessed by random amplified polymorphic DNA and simple sequence repeat markers. Genet Res Crop Evol 51:????.

    Google Scholar 

  • Nei, M., 1973. Analysis of gene diversity in subdivided populations. Proc Natl Acad Sci USA 70: 3321–3323.

    Article  PubMed  CAS  Google Scholar 

  • Pitrat, M., 1994. Linkage groups in Cucumis melo L. Cucurbit Genet Coop Rep 17: 148–149.

    Google Scholar 

  • Robinson, R.W & D.S Decker-Walters, 1997. Cucurbits Cab International, New York.

  • Rohlf, J.F., 1997. NTSYS-pc. Numerical Taxonomy and Multivariate Analysis System, version 1.8. Exeter Software, New York.

  • Rubatzky, V.E. & M. Yamaguchi, 1997. World vegetables: Principles, Production, and Nutritive Values. Chapman and Hall, New York.

    Google Scholar 

  • SAS Institute, 1992. SAS/STAT User's Guide, Release 6.03 ed., p. 1028. SAS Institute, Cary, NC.

    Google Scholar 

  • Silberstein, L., I.Kovalski, R.G. Huang, K. Anagnostu, M.M.K. Jahn & R. Perl-Treves, 1999. Molecular variation in melon (Cucumis melo L.) as revealed by RFLP and RAPD markers. Sci Hort 79: 101–111.

    Article  CAS  Google Scholar 

  • Spooner, D.M., J. Tivang, J. Nienhuis, J.T. Miller, D.S. Douches & M. Contreras, 1996. Comparison of four molecular markers in measuring relationships among wild potato relatives Solanum section Etuberosum (subgenus Potato). Theor Appl Genet 92: 532–540.

    Article  CAS  Google Scholar 

  • Staub, J.E., 2001. Inheritance of RAPD markers in Melon (Cucumis melo L.). Cucurbit Genet Coop Rep 24: 29–32.

    Google Scholar 

  • Staub, J.E., J. Bacher & K. Poetter, 1996. Factors affecting the application of random amplified polymorphic DNAs in cucumber (Cucumis sativus L.). HortScience 31: 262–266.

    CAS  Google Scholar 

  • Staub, J.E., J. Box, V. Meglic, T.F. Horejsi & J.D. McCreight, 1997. Comparison of isozyme and random amplified polymorphicDNA data for determining intraspecific variation in Cucumis. Gen Res Crop Evol 44: 257–269.

    Article  Google Scholar 

  • Staub, J.E., Y. Danin-Poleg, G. Fazio, T. Horejsi, N. Reis & N. Katzir, 2000. Comparison analysis of cultivated melon groups (Cucumis melo L.) using random amplified polymorphic DNA and simple sequence repeat markers. Euphytica 115: 225–241.

    Article  CAS  Google Scholar 

  • Stepansky, A., I. Kovalski & R. Perl-Treves, 1999. Intraspecific classification of melons (Cucumis melo L.) in viewof their phenotypic and molecular variation. Plant Syst Evol 217: 313–332.

    Article  CAS  Google Scholar 

  • Whitaker, T.W. & D.W. Davis. 1962. Cucurbits: botany, cultivation and utilization. Interscience Pub., New York.

    Google Scholar 

  • Whitaker, T.W. & W.P. Bemis, 1976. Cucurbits, Cucumis, Citrullus, Cucurbita, Lagenaria (Cucurbitaceae). In:N.W. Simmonds (Ed.), Evolution of Crop Plants, pp. 64–69. Longrams, New York.

    Google Scholar 

  • Yeh, F.C., R.C. Yang, T. Boiley, Z.H. Ye & J.X. Mao, 1997. POPGENE, the User-Friendly Shareware for Population Genetic Analysis. Molecular Biology and Biotechnology Center, University of Alberta, Canada.

    Google Scholar 

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Authors and Affiliations

  1. U.S. Department of Agriculture, Agricultural Research Service, Vegetable Crops Research Unit, Department of Horticulture, 1575 Linden Dr., University of Wisconsin, Madison, WI, 53706, U.S.A.

    Jack E. Staub & Ana I. López-Sesé

  2. Technical Educational Institute, Heraklion, Crete, 71500, Greece

    Nikolaos Fanourakis

Authors
  1. Jack E. Staub
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  2. Ana I. López-Sesé
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  3. Nikolaos Fanourakis
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Staub, J.E., López-Sesé, A.I. & Fanourakis, N. Diversity among melon landraces (Cucumis melo L.) from Greece and their genetic relationships with other melon germplasm of diverse origins. Euphytica 136, 151–166 (2004). https://doi.org/10.1023/B:EUPH.0000030667.63614.bd

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