Abstract
Glycoalkaloids are secondary metabolites characterised by an undesirable taste, and known to be toxic when consumed in large quantities. Their content in potato tubers should therefore be selected against and DNA markers can significantly facilitate such a process. This study was designed to describe the genetic control of the total content of glycoalkaloids (TGA) in the potato tuber and to enhance the development of DNA markers. A segregating population was constructed from tetraploid potatoclones, which were characterised by divergent TGA-content. The population had a high-TGA parent, LT7, and a low-TGA parent, NT8. We used 342 Inter-Simple Sequence Repeat (ISSR) single primers or two such primer combinations and 36 Cleaved Amplified Polymorphic Sequence (CAPS)primer pairs, digested with various restriction enzymes, to amplify random PCR products. Parental clones of both populations were used to identify polymorphic PCR products for further linkage analyses with TGA-content. In these analyses, one single ISSR marker was found to be significantly associated with TGA-content. A multiple regression analysis was also carried out using a ‘stepwise’ procedure. In this regression analysis TGA-content was the dependent variable whereas the polymorphic PCR products and all possible two-way interactions among them were the independent variables. The resulting best model consisted of an interaction between two loci in addition to a single locus effect. This interaction suggests that the expression of TGA-content is partially modulated by two interacting loci.
Similar content being viewed by others
References
Bingham, E.T., R.W. Groose, D.R. Woodfield & K.K. Kidwell, 1994. Complementary gene interactions in alfalfa are greater in autotetraploids than diploids. Crop Sci 34: 823-829.
Bonierbale, M.W., R.L. Plaisted & S.D. Tanksley, 1993. A test of the maximum heterozygosity hypothesis using molecular markers in tetraploid potatoes. Theor Appl Genet 86: 481-491.
Bouarte-Medina, T., E. Fogelman, E. Chani, A.R. Miller, I. Levin, D. Levy & R.E. Veilleux, 2002. Identification of molecular markers associated with leptine in reciprocal backcross families of diploid potato. Theor Appl Genet 105: 1010-1018.
Bradshaw, J.E., C.A. Hackett, R.C. Meyer, D. Milbourne, J.W. Mcnicol, M.S. Phillips & R. Waugh, 1998. Identification of AFLP and SSR markers associated with quantitative resistance to Globodera pallida (Stone) in tetraploid potato (Solanum tuberosum subsp. tuberosum) with a view to marker-assisted selection. Theor Appl Genet 97: 202-210.
Caligari, P.D.S., 1992. Breeding new varieties. In: P. Harris (Ed.), The Potato Crop, 2nd edn., pp. 334-372. Chapman & Hall, London.
Coupland, G., 1995. Genetic and environmental control of flowering time in Arabidopsis. Trends Genet 11: 393-397.
Craenen, K. & R. Ortiz, 1996. Effect of the black sigatoka resistance locus bs1 and ploidy level on fruit and bunch traits of plantain-banana hybrids. Euphytica 97: 97-101.
Dimenstein, L., N. Lisker, N. Kedar & D. Levy, 1997. Changes in the content of steroidal glycoalkaloids in potato tubers grown in the field and in the greenhouse under different conditions of light, temperature and daylength. Physiol Mol Plant Pathol 50: 391-402.
Eshed, Y. & D. Zamir, 1996. Less-than-additive epistatic interactions of quantitative trait loci in tomato. Genetics 143: 1807-1817.
Freyre, R., S. Warnke, B. Sosinski & D.S. Douches, 1994. Quantitative trait locus analysis of tuber dormancy in diploid potato (Solanum spp.). Theor Appl Genet 89: 474-480.
Fulton, M.T., J. Chunwongse & S.D. Tanksley, 1995. Microprep protocol for extraction of DNA from tomato and other herbaceous plants. Plant Mol Biol Rep 13: 207-209.
Gebhardt C., E. Ritter, A. Barone, T. Debener, B. Walkemeier, U. Schachtschabel, H. Kaufmann, R.D. Thompson, M.W. Bonierbale, M.W. Ganal, S.D. Tanksley, F. Salamini, 1991. RFLP maps of potato and their alignment with the homoeologous tomato genome. Theor Appl Genet 83: 49-57.
International Potato Center, 1982. Maintenance, utilization, and distribution of tuber-bearing solanums; Thrust II. In: Annual Report CIP 1982, pp. 23-32. International Potato Center (CIP), Lima.
Kidwell, K.K., E.T. Bingham, D.R. Woodfield & T.C. Osborn, 1994. Relationships among genetic distance, forage yield and hetrozygosity in isogenic diploid and tetraploid alfalfa populations. Theor Appl Genet 89: 323-328.
Kreike, C.M., A.A. Kok-Westeneng, J.H. Vinke & W.J. Stiekema, 1996. Mapping of QTLs involved in nematode resistance, tuber yield and root development in Solanum sp. Theor Appl Genet 92: 463-470.
Kutchan, T.M., 1995. Alkaloid biosynthesis-The basis for metabolic engineering of medical plants. Plant Cell 7: 1059-1070.
Levin, I., N. Gilboa, E. Yeselson & S. Shen, 2000. Fgr, a major locus that modulates the fructose to glucose ratio in mature tomato fruits. Theor Appl Genet 100: 256-262.
Levy, D., E. Kastenbaum & Y. Itzhak, 1991. Evaluation of parents and selection for heat tolerance in the early generations of a potato (Solanum tuberosum L.) breeding program. Theor Appl Genet 82: 130-136.
Levy, D., A. Meiri, A. Carmi & A. Nachmias, 1995. Improvement of potato productivity in developing countries. Research and Development project between the Netherlands and Israel Report for 1994-1995. The Volcani Center, Bet Dagan.
Levy, D., Y. Itzhak, E. Fogelman, E. Margalit & R.E. Veilleux, 2001. Ori, Idit, Zohar and Zahov: tablestock and chipstock cultivars bred for adaptation to Israel. Amer J of Potato Res 78: 167-173.
McGregor, C.E., C.A. Lambert, M.M. Greyling, J.L. Louw & L. Warnich, 2000. A comparitive assessment of DNA fingerprinting techniques (RAPD, ISSR, AFLP and SSR) in tetraploid potato (Solanum tuberosum L.) germplasm. Euphytica 113: 135-144.
Meyer, R.C., D. Milbourne, C.A. Hackett, J.E. Bradshaw, J.W. Mc-Nicol & R. Waugh, 1998. Linkage analysis in tetraploid potato and association of markers with quantitative resistance to late blight (Phytophthora infestance). Mol Gen Genet 259: 150-160.
Oberhagemann, P., C. Chatot-Balandras, R. Schafer-Pregl, D. Wegener, C. Palomino, F. Salamini, E. Bonnel & C. Gebhardt, 1999. A genetic analysis of quantitative resistance to late blight in potato: towards marker-assisted selection. Mol Breed 5: 399-415.
Prevost, A. & M.J. Wilkinson, 1999. A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivars. Theor Appl Genet 98: 107-112.
Ronning, C.M., J.R. Stommel, S.P. Kowalski, L.L. Sanford, R.S. Kobayashi & O. Pineada, 1999. Identification of molecular markers associated with leptine production in a population of Solanum chacoense Bitter. Theor Appl Genet 98: 39-46.
Rouppe van der Voort, J.N.A.M., W. Lindeman, R. Folkertsma, R. Hutten, H. Overmars, E. van der Vossen, E. Jacobsen & J. Bakker, 1998. A QTL for broad-spectrum resistance to cyst nematode species (Globodera spp.) maps to a resistance gene cluster in Potato. Theor Appl Genet 96: 654-661.
Rouppe van der Voort, J.N.A.M., G.J.W. Janssen, H. Overmars, P.M. van Zandvoort, A. van Norel, O.E. Scholten, R. Janssen & J. Bakker, 1999. Development of a PCR-based selection assay for root-knot nematode resistance (Rmc1) by a comparative analysis of the Solanum bulbocastanum and S. tuberosum genome. Euphytica 106: 187-195.
Salimath, S.S., A.C. de Olivera, I.D. Goodwin & J.L. Bennetzen, 1995. Assessment of genomic origins and genetic diversity in the genus Eleusine with DNA markers. Genome 38: 757-763.
SAS Institute, 1995. JMP Statistical Discovery Software, Version 3.1.5. SAS Institute Inc., Cary NC.
Šimko, I., D. Vreugdenhil, C.S. Jung & G.D. May, 1999. Similarities of QTLs detected for in vitro and greenhouse development of potato plants. Mol Breed 5: 417-428.
Staub, J.E. & F.C. Serquen, 1996. Genetic markers, map construction, and their application in plant breeding. HortScience 31: 729-741.
Storey, R.M.J. & H.V. Davies, 1992. Tuber quality. In: P. Harris (Ed.), The Potato Crop, 2nd edn., pp 507-569. Chapman & Hall, London.
Tai, G.C.C., 1982. Estimation of double reduction and genetic parameters of autotetraploids. Heredity 49: 63-70.
Tenkouano, A., J.H. Crouch, H.K. Crouch, D. Vuylsteke & R. Ortiz, 1999. Comparison of DNA marker and pedigree-based methods of genetic analysis in plantain and banana (Musa spp.) clones. II Predicting hybrid performance. Theor Appl Genet 98: 69-75.
Van Dam, J., 2002. Genetic Characterisation of Agronomic and Morphological Traits and the Development of DNA Markers associated with Total Glycoalkaloid Content in the Tubers of Tetraploid Potato (Solanum tuberosum L.). PhD Thesis, Wageningen University, Wageningen, The Netherlands.
Van Dam, J., I. Levin, P.C. Struik & D. Levy, 1999. Genetic characterisation of tetraploid potato (Solanum tuberosum L.) emphasising genetic control of total glycoalkaloid content in the tubers. Euphytica 110: 67-76.
Van Gelder, W.M.J., 1989. Steroidal Glycoalkaloids in Solanum Species: Consequences for Potato Breeding and Food Safety. PhD Thesis, Wageningen Agricultural University, Wageningen, The Netherlands.
Van Gelder, W.M.J. & J.J.C. Scheffer, 1991. Transmission of steroidal glycoalkaloids from Solanum vernei to the cultivated potato. Phytochemistry 30: 165-168.
Veilleux, R.E., M.M. Paz & D. Levy, 1997. Potato germplasm development for warm climates: genetic enhancement of tolerant to heat stress. Euphytica 98: 83-92.
Wu, P., G. Zhang, H. Huang & J.K. Ladha, J.K. 1995. Non-allelic interaction conditioning spikelet sterility in an F2 population of indica/japonica cross in rice. Theor Appl Genet 91: 825-829.
Yencho, G.C., S.P. Kowalski, R.S. Kobayashi, S.L. Sinden, M.W. Bonierbale & K.L. Deahl, 1998. QTL mapping of foliar glycoalkaloid aglycones in Solanum tuberosum × S. berthaultii potato progenies: quantitative variation and plant secondary metabolism. Theor Appl Genet 97: 563-574.
Zhang, Y. & J.R. Stommel, 2001. Development of SCAR and CAPS Markers Linked to the Beta Gene in Tomato. Crop Science 41: 1602-1608.
Ziêtkiewicz, E., A. Rafalski & D. Labuda, 1994. Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics 20: 176-183.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
van Dam, J., Levin, I., Struik, P.C. et al. Identification of epistatic interaction affecting glycoalkaloid content in tubers of tetraploid potato (Solanum tuberosum L.). Euphytica 134, 353–360 (2003). https://doi.org/10.1023/B:EUPH.0000004985.01425.65
Issue Date:
DOI: https://doi.org/10.1023/B:EUPH.0000004985.01425.65