Abstract
The effects of growth conditions, trace metals, carbon and nitrogen source on ochratoxin A (OTA) production by Aspergillus ochraceus NRRL 3174 were investigated in submerged liquid culture medium. No correlation between biomass production and OTA synthesis was observed. OTA production depended on the initial pH and was influenced by changing the pH of the culture medium. This effect was not due to differences in the availability of metal ions. At pH 6.5, 0.2 mg l−1 Zn increased biomass and OTA production by 50%. Fe at 0.12 mg l−1 resulted in a 40% decrease in the amount of OTA/biomass although the quantity of biomass was not affected. Amounts of OTA produced per biomass increased with decreasing glucose concentrations. Lactose triggered the highest amounts of OTA/biomass despite low yields of biomass. NO3 − stimulated the amount of OTA/biomass by 25% and NH4 + reduced the amount of OTA/biomass by 25% compared with NH4NO3 as nitrogen source.
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References
Adye J and Mateles RI (1964) Incorporation of labeled compounds in aflatoxins. Biochimica and Biophysica Acta 86: 418-420
Aziz NH and Moussa LAE (1997) Influence of white light, near-UV irradiation and other environmental conditions on production of aflatoxin B1 by Aspergillus flavus and ochratoxin A by Aspergillus ochraceus. Nahrung 41: 150-154
Bennett and Christensen (1983)
Castella G, Larsen TO, Cabañes J, Schmidt H, Alboresi A, Niessen L, Färber P and Geisen R (2002) Molecular characterization of ochratoxin A producing strains of the genus Penicillium. Systematic and Applied Microbiology 25: 74-83
Davis ND, Searcy JW and Diener UL (1969) Production of ochratoxin A by Aspergillus ochraceus in a semi synthetic medium. Applied Microbiology 17: 742-744
Edwards SG, O'Callaghan J and Dobson ADW (2002) PCR-based detection and quantification of mycotoxigenic fungi. Mycological Research 106: 1005-1025
Ferreira NP (1967) Recent advances in research on Ochratoxin. Part 2: Microbiological aspects. In: Mateles RAI, and Wogan P (eds) Biochemistry of some food borne microbial toxins (pp 157–168) MIT Press, Cambridge, UK
Ferreira NP (1968) The effect of amino acids on the production of ochratoxin A in chemically defined media. Antonie van Leeuwenhoek 34: 433-440
Ferreira NP and Pitout MJ (1969) The biogenesis of ochratoxin. Journaal van die Suid-Afrikaanse Chemiese Instituut XXII: 1-8
Filtenborg O, Frisvad JC and Svendsen, JA (1983) Simple screening method for molds producing intracellular mycotoxins in pure cultures. Applied and Environmental Microbiology 45: 581-585
Filtenborg O, Frisvad JC and Thrane U (1990) The significance of yeast extract composition on metabolite production in Penicillium. In: Samson RA and Pitt JI (eds) Modern Concepts in Penicillium and Aspergillus Classification (pp 433–441) Plenum Press, New York, USA
Harris JP and Mantle PG (2001) Biosynthesis of ochratoxins by Aspergillus ochraceus. Phytochemistry 58: 709-716
Harwig (1974)
Huff WE and Hamilton PB (1979) Mycotoxins-their biosynthesis in fungi: Ochratoxins-metabolites of combined pathways. Journal of Food Protection 42: 815-820
Joosten HMLJ, Goetz J, Pittet A, Schellenberg M and Bucheli P (2001) Production of ochratoxin A by Aspergillus carbonarius on coffee cherries. International Journal of Food Microbiology 65: 39-44
Lai M, Semeniuk G and Hesseltine CW (1970) Conditions for production of ochratoxin A by Aspergillus species in a synthetic medium. Applied Microbiology 19: 542-544
Larsen TO, Svendsen A and Smedsgaard J (2001) Biochemical characterization of ochratoxin A-producing strains of the genus Penicillium. Applied and Environmental Microbiology 67: 3630-3635
Lee et al. (1986)
Leiach and Papa (1974)
Lemke et al. (1989)
Mayne et al. (1971)
Moss M (1998) Recent studies of mycotoxins. Journal of Applied Microbiology 84: 62-76
Niessen and Vogel (1997)
Röschenthaler et al. (1984)
Schmidt H, Ehrmann M, Vogel RF, Taniwaki MH and Niessen L (2003) Molecular typing of Aspergillus ochraceus and construction of species specific SCAR-primers based on AFLP. Systematic and Applied Microbiology 26: 434-438
Steele JA, Davis ND and Diener UL (1973) Effect of Zn, copper and Fe on ochratoxin A production. Applied Microbiology 25: 847-849
Steyn P and Holzapfel WH (1970) The biosynthesis of the ochratoxins, metabolites of Aspergillus ochraceus. Phytochemistry 9: 1977-1983
Varga J, Kevei È, Rinyu E, Téren J and Kozakiewicz Z (1996) Ochratoxin production by Aspergillus species. Applied and Environmental Microbiology 62: 4461-4464
Wei R-D, Strong FM and Smalley EB (1971) Incorporation of chlorine-36 into ochratoxin A. Applied Microbiology 22: 276-277
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Mühlencoert, E., Mayer, I., Zapf, M.W. et al. Production of Ochratoxin A by Aspergillus Ochraceus . European Journal of Plant Pathology 110, 651–659 (2004). https://doi.org/10.1023/B:EJPP.0000032404.71695.6b
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DOI: https://doi.org/10.1023/B:EJPP.0000032404.71695.6b