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Identification of Growth Stage Specific Transcript Profiles in Fusarium Proliferatum (Gibberella Fujikuroi, Mating Population D) by cDNA-AFLP Analysis

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Abstract

A cDNA-AFLP approach was developed to identify differentially regulated genes in mycotoxin-producing and non-producing growth stages of Fusarium proliferatum(Gibberella fujikuori Mating Population-D). Using 160 PCR-primer combinations, a number of fragments which showed strikingly different intensities depending on growth stage were cloned, subjected to Northern analysis and sequenced. Two fragments, amplified from genes up-regulated during spore germination gave significant sequence homology to an amino acid transporter gene from Neurospora crassa and a GAL4-like transcriptional activator, respectively. Among the cDNAs derived from late growth stage transcripts, fragments of genes involved in polyol metabolism and cell cycle regulation, respectively were identified. Additional cDNAs (without significant homologies to known sequences) were also isolated and these clearly differentiated between mycotoxin-producing and non-producing growth stages of the fungus.

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References

  • Abdalla MY, Al-Rokibah A, Moretti A and Mulé G (2000) Pathogenicity of toxigenic Fusarium proliferatum from date palm in Saudi Arabia. Plant Disease 84: 321-324

    Google Scholar 

  • Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Millwer W and Lipman DJ (1997) Gapped BLAST and PSI-BLAST: A new generation of protein database search programs. Nucleic Acids Research 25: 3389-3402

    Article  CAS  PubMed  Google Scholar 

  • Barna-Vetró I, Szabó E, Fazekas B and Solti L (2000) Development of a sensitive ELISA for the determination of fumonisin B1 in cereals. Journal of Agricultural and Food Chemistry 48: 2821-2825

    PubMed  Google Scholar 

  • Bailey LA and Ebbole DJ (1998) The fluffy gene of Neurospora crassa encodes a Gal4p-type C6 zinc cluster protein required for conidial development. Genetics 148: 1813-1820

    CAS  PubMed  Google Scholar 

  • Britz H, Coutinho TA, Wingfield MJ, Marasas WFO, Gordon TJ and Leslie JF (1999) Fusarium subglutinans f.sp. pini represents a distinct mating type population in the Gibberella fujikuroi species complex. Applied and Environmental Microbiology 65: 1198-1201

    CAS  PubMed  Google Scholar 

  • Cappellini R and Peterson JL (1965) Macroconidium formation in submerged cultures by a non-sporulating strain of Gibberella zeae. Mycologia 57: 962-966

    Google Scholar 

  • Dantzer WR, Pometto AL 3rd and Murphy PA (1996) Fumonisin B1 production by Fusarium proliferatum strain M5in a modified Myro liquid medium. Natural Toxins 4: 168-173

    CAS  PubMed  Google Scholar 

  • Desjardins AE, Plattner RD and Nelson PE (1997) Production of fumonisin B1 and moniliformin by Gibberella fujikuroi from rice and from various geographical areas. Applied and Environmental Microbiology 63: 1838-1842

    CAS  Google Scholar 

  • Devereux J, Haeberli P and Smithies O (1984) A comprehensive set of sequence analysis programs for VAX. Nucleic Acids Research 12: 387-395

    CAS  PubMed  Google Scholar 

  • Dufresne M, Perfect S, Pellier A-L, Bailey JA and Langin T (2000) A GAL4-like protein is involved in the switch between biotrophic and necrotrophic phases of the infection process of Colletotrichum lindemuthianum on common bean. Plant Cell 12: 1578-1589

    Article  Google Scholar 

  • Elmer WH (1995) A single mating population of Gibberella fujikuroi (Fusarium proliferatum) predominates in asparagus fields in Connecticut, Massachusetts and Michigan. Mycologia 87: 68-71

    Google Scholar 

  • Gow NAR and Gadd GM (1995) The Growing Fungus. Chapman and Hall, London

    Google Scholar 

  • Hocking AD and Norton JR (1983) Natural abundance 13C under magnetic resonance studies on the internal solutes of xerophilic fungi. Journal of General Microbiology 129: 2915-2925

    CAS  Google Scholar 

  • Klittich CJR, Leslie JF, Nelson PE and Marasas WFO (1997) Fusarium thapsinum (Gibberella thapsina): A new species in section Liseola from sorghum. Mycologia 89: 643-652

    Google Scholar 

  • Leslie JF (1991) Mating populations in Gibberella fujikuroi (Fusarium section Liseola). Phytopathology 81: 1058-1060

    Google Scholar 

  • Leslie JF (1995) Gibberella fujikuroi: Available populations and variable traits. Canadian Journal of Botany 73: S282-S291

    Google Scholar 

  • Leslie, JF, Plattner RD, Desjardins AE and Klittich CJR (1992) Fumonisin B1 production by strains from different mating populations of Gibberella fujikuroi (Fusarium section Liseola). Phytopathology 82: 341-345

    CAS  Google Scholar 

  • Logrieco A, Moretti A, Ritieni A, Bottalico A and Corda P (1995) Occurrence and toxigenicity of Fusarium proliferatum from preharvest maize ear rot and associated mycotoxins in Italy. Plant Disease 79: 727-731

    Google Scholar 

  • Luke MM, Della Seta F, Di Como CJ, Sugimoto H, Kobayashi R and Arndt KT (1996) The SAPs, a new family of proteins, associate and function positively with the SIT4 phosphatase. Molecular and Cellular Biology 16: 2744-2755

    CAS  PubMed  Google Scholar 

  • Marasas WFO, Thiela PG, Rabie CJ, Nelson PE and Toussoun TA (1986) Moniliformin production in Fusarium section Liseola. Mycologia 78: 242-247

    CAS  Google Scholar 

  • Moretti A, Logrieco A, Bottalico A, Ritieni A, Fogliano A and Randazzo G (1997a) Diversity in beauvericin and fusaproliferin production by different populations of Gibberella fujikuroi (Fusarium section Liseola). Sydowia 48: 44-56

    Google Scholar 

  • Moretti A, Logrieco A, Doko B, Frisullo S, Visconti A and Bottalico A (1997b) Fusarium proliferatum from asparagus in Italy: Occurrence, fertility and toxigenicity. Cereal Research Communications 25: 785-786

    Google Scholar 

  • Moretti A, Mulé G, Perrone G and Logrieco A (1998) Genetic and toxigenic characterization of Fusarium moniliforme and F. proliferatum from various plants. In: Book of Abstracts (pp. 166) Sixth International Mycological Congress, Jerusalem, Israel, 23–28 August

  • Nelson PE, Desjardins AE and Plattner RD (1993) Fumonisins, mycotoxins produced by Fusarium species: Biology, chemistry, and significance. Annual Review of Phytopathology 31: 233-252

    Article  Google Scholar 

  • Nirenberg H and O'Donnell K (1998) New Fusarium species and combinations within the Gibberella fujikuroi species complex. Mycologia 90: 434-458

    Google Scholar 

  • Proctor RH, Brown DW, Plattner RD and Desjardins AE (2003) Co-expression of 15 contiguous genes delineates a fumonisin biosynthetic gene cluster in Gibberella moniliformis. Fungal Genetics and Biology 38: 237-249

    Article  CAS  PubMed  Google Scholar 

  • Proctor RH, Desjardins AE, Plattner RD and Hohn TM (1999) A polyketide synthase gene required for biosynthesis of fumonisin mycotoxins in Gibberella fujikuroi mating population A. Fungal Genetics and Biology 27: 100-112

    Article  CAS  PubMed  Google Scholar 

  • Sambrook S, Fritsch EF and Maniatis T (1989) Molecular Cloning. A Laboratory Manual. Cold Spring Harbor Laboratory Press, New York

    Google Scholar 

  • Seo J-A, Proctor RH and Plattner RD (2001) Characterization of four clustered and coregulated genes associated with fumonisin biosynthesis in Fusarium verticillioides. Fungal Genetics and Biology 34: 155-165

    Article  CAS  PubMed  Google Scholar 

  • Shaw SF, Elliott V, Mansour IM and Leslie JF (1993) Genetic diversity in Gibberella fujikuroi (Fusarium section Liseola) from bananas. Fungal Genetics Newsletter 40A: Abstract 25

  • Shim W-B and Woloshuk CP (2001) Regulation of fumonisin B1 biosynthesis and conidiation in Fusarium verticillioides by a cyclin-like (C-type) gene, FCC1. Applied and Environmental Microbiology 67: 1607-1612

    Article  CAS  PubMed  Google Scholar 

  • Vos P, Hogers R, Bleeker M, Reijans M, van-de LT, Hornos M, Frijters A, Pot J, Peleman J, Kuiper M and Zabeau M (1995) AFLP: A new technique for DNA fingerprinting. Nucleic Acids Research 23: 4407-4414

    CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Agricultural Biotechnology and Microbiology, Group of Mycology, Hungarian Academy of Sciences, Szent István University, Páter K. u. 1., Gödöllö, H-2103, Hungary

    A. Jeney, E. Béki & L. Hornok

  2. Agricultural Biotechnology Center, Szent-Györgyi A. u. 4., Gödöllö, H-2100, Hungary

    A. Jeney, E. Béki & L. Hornok

  3. Institute of Science and Food Production, C.N.R., Viale Einaudi 51, 70125, Bari, Italy

    G. Mulè

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  1. A. Jeney
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  2. E. Béki
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  3. G. Mulè
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  4. L. Hornok
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Correspondence to L. Hornok.

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Jeney, A., Béki, E., Mulè, G. et al. Identification of Growth Stage Specific Transcript Profiles in Fusarium Proliferatum (Gibberella Fujikuroi, Mating Population D) by cDNA-AFLP Analysis. European Journal of Plant Pathology 110, 619–625 (2004). https://doi.org/10.1023/B:EJPP.0000032401.78288.d2

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  • DOI: https://doi.org/10.1023/B:EJPP.0000032401.78288.d2

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