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Development of PCR Primers for a New Fusarium oxysporum Pathogenic on Paris Daisy (Argyranthemum frutescens L.)

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Abstract

The inverse PCR technique was applied to clone genomic DNA flanking insertion sites of sequences homologous to the transposable element Fot1 in the genome of a new pathogenic isolate of Fusarium oxysporum obtained from wilted Argyranthemum frutescens (Paris daisy). Based on the genomic flanking regions, a primer was designed which when paired to a second primer matching the Fot1 sequence allowed detection of this pathogen by PCR. The primer pair Mg5/Mg6 could specifically identify nine tested isolates of F. oxysporum from A. frutescens, when fungal genomic DNA was used as template. Moreover, the primer pair Mg5/Mg6 allowed successful detection of the pathogen in stem and root tissue from asymptomatic plants that were artificially inoculated with a representative isolate of F. oxysporum from A. frutescens.

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Authors and Affiliations

  1. AGRINNOVA – Centre of Competence for the Innovation in the agro-environmental field, University of Torino, Via Leonardo da Vinci 44, I-10095, Grugliasco (Torino), Italy (Phone

    Matias Pasquali, Alberto Acquadro, Maria Lodovica Gullino & Angelo Garibaldi

  2. Department of Plant Protection and Center of Excellence for Biotechnology Development and Biodiversity Research, University of Sassari, Via E. De Nicola 9, I-07100, Sassari, Italy

    Virgilio Balmas & Quirico Migheli

Authors
  1. Matias Pasquali
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  2. Alberto Acquadro
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  3. Virgilio Balmas
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  4. Quirico Migheli
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  5. Maria Lodovica Gullino
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  6. Angelo Garibaldi
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Pasquali, M., Acquadro, A., Balmas, V. et al. Development of PCR Primers for a New Fusarium oxysporum Pathogenic on Paris Daisy (Argyranthemum frutescens L.). European Journal of Plant Pathology 110, 7–11 (2004). https://doi.org/10.1023/B:EJPP.0000010141.37327.d0

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  • DOI: https://doi.org/10.1023/B:EJPP.0000010141.37327.d0

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