Abstract
Fusarium culmorum is a phytopathogenic, toxigenic fungus causing seedling diseases, foot rot and head blight of cereals. For estimating competition effects in mixtures of two single-spore isolates, two winter rye single crosses were tested with either four isolates individually or four 1 : 1 mixtures of the same isolates in six field environments. Two isolates (FC46, FC64) were highly aggressive deoxynivalenol (DON) and 3-acetyl DON-producers, the other two (FC30, FC71) were medium aggressive nivalenol-producers. Rye heads were inoculated during flowering with conidia of pairs of isolates expressing similar (FC46 + FC64, FC30 + FC71) or contrary (FC46 + FC71, FC30 + FC64) levels of aggressiveness and similar or different concentrations and chemotypes of mycotoxins, respectively. Head blight rating and yield components relative to the non-inoculated plots were recorded as aggressiveness traits. Additionally, mycotoxin concentrations were measured in the rye grain. Random pathogen samples were re-isolated from heads at the onset of symptom development and analysed by molecular markers (RAPD–PCR) in one environment. Aggressiveness of the isolate mixtures was significantly lower than that of the isolates applied individually on both rye genotypes. Similarly, mycotoxin concentrations were significantly lower in the mixtures in seven out of eleven comparisons. Among the re-isolates, the component genotypes of a mixture significantly deviated from the inoculated 1 : 1 ratio when a particular isolate (FC46) was present in the mixture. This isolate displayed a superior competitive ability irrespective of the aggressiveness or mycotoxin profile of the mixing partner illustrating that pathogenic fitness is caused by additional factors that have not, as yet, been identified.
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References
Bowden JL and Leslie JF (1994) Diversity of Gibberella zeae at small spatial scales. Phytopathology 84: 1140 (abstract)
Burgess LW and Griffin DM (1967) Competitive saprophytic colonization of wheat straw. Annals of Applied Biology 60: 137-142
Cochran WG and Cox GM (1957) Experimental Designs, 2nd edn. John Wiley & Sons, New York
Cuero R, Smith JE and Lacey J (1988) Mycotoxin formation by Aspergillus flavus and Fusarium graminearum in irradiated maize grains in the presence of other fungi. Journal of Food Protection 51: 452-456
D'Mello JPF, Placinta CM and Macdonald AMC (1999) Fusarium mycotoxins: A review of global implications for animal health, welfare and productivity. Animal Feed Science and Technology 80: 183-205
Fehr WR (1987) Principles of Cultivar Development, Vol 1, Theory and Technique. Macmillan Publishing Company, New York, USA
Gang G, Miedaner T, Schuhmacher U, Schollenberger M and Geiger HH (1998) Deoxynivalenol and nivalenol production by Fusarium culmorum isolates differing in aggressiveness toward winter rye. Phytopathology 88: 879-884
Gerlach W and Nirenberg H (1982) The genus Fusarium-a pictorial atlas. Mitteilungen aus der Biologischen Bundesanstalt für Land-und Forstwirtschaft Berlin-Dahlem 209: 1-406
Jenkinson P and Parry DW (1994) Splash dispersal of conidia of Fusarium culmorum and Fusarium avenaceum. Mycological Research 98: 506-510
Miedaner T, Gang G and Geiger HH (1996a) Quantitative-genetic basis of aggressiveness of 42 isolates of Fusarium culmorum for winter rye head blight. Plant Disease 80: 500-504
Miedaner T, Gang G and Geiger HH (1996b) Genetics of resistance and aggressiveness in the winter rye/Fusarium culmorum head blight pathosystem. Vorträge für Pflanzenzüchtung 35: 165-174
Miedaner T, Schilling AG and Geiger HH (2001a) Molecular genetic diversity and variation for aggressiveness in populations of Fusarium graminearum and Fusarium culmorum sampled from wheat fields in different countries. Journal of Phytopathology 149: 641-648
Miedaner T, Reinbrecht C, Lauber U, Schollenberger M and Geiger HH (2001b) Effects of genotype and genotype × environment interaction on deoxynivalenol accumulation and resistance to Fusarium head blight in rye, triticale, and wheat. Plant Breeding 120: 97-105
Möller EM, Bahnweg G, Sandermann H and Geiger HH (1992) A simple and efficient protocol for isolation of high molecular weight DNA from filamentous fungi, fruit bodies, and infected plant tissues. Nucleic Acids Research 20: 6115-6116
Muthomi JW, Schütze A, Dehne HW, Mutitu EW and Oerke EC (2000) Characterization of Fusarium culmorum isolates by mycotoxin production and aggressiveness to winter wheat. Journal of Plant Diseases and Protection 107: 113-123
Nirenberg H (1981) A simplified method for identifying Fusarium spp. occurring on wheat. Canadian Journal of Botany 59: 1599-1609
Parry DW, Jenkinson P and McLeod L (1995) Fusarium ear blight (scab) in small grain cereals-a review. Plant Pathology 44: 207-238
Placinta CM, D'Mello JPF and Macdonald AMC (1999) A review of worldwide contamination of cereal grains and animal feed with Fusarium mycotoxins. Animal Feed Science and Technology 78: 21-37
Ramakrishna N, Lacey J and Smith JE (1996) The effects of fungal competition on colonization of barley grain by Fusarium sporotrichioides on T-2 toxin formation. Food Additives and Contaminants 8: 939-948
Reid LM, Nicol RW, Ouellet T, Savard M, Miller JD, Young JC, Stewart DW and Schaafsma AW (1999) Interaction of Fusarium graminearum and F. moniliforme in maize ears: Disease progress, fungal biomass, and mycotoxin accumulation. Phytopathology 89: 1028-1037
Schilling AG (1996) Characterization and Differentiation of the Cereal Pathogens Fusarium culmorum and F. graminearum by PCR-based Molecular Markers. U.E. Grauer, Stuttgart, Germany
Schilling AG, Möller EM and Geiger HH (1996) Polymerase chain reaction-based assays for species-specific detection of Fusarium culmorum, F. graminearum and F. avenaceum. Phytopathology 86: 515-522
Schollenberger M, Lauber U, Terry Jara H, Suchy S, Drochner W and Müller HM (1998) Determination of eight trichothecenes by gas chromatography-mass spectrometry after sample clean-up by a two-stage solid-phase extraction. Journal of Chromatography A 815: 123-132
Snedecor GW and Cochran WG (1989) Statistical Methods, 8th edn. Iowa State University, Ames
Tanaka T, Hasegawa A, Matsuki Y, Lee US and Ueno Y (1985) Rapid and sensitive determination of zearalenone in cereals by high-performance liquid chromatography with fluorescence detection. Journal of Chromatography 328: 271-278
van Eeuwijk FA, Mesterházy Á, Kling CI, Ruckenbauer P, Saur L, Bürstmayr H, Lemmens M, Keizer LCP, Maurin N and Snijders CHA (1995) Assessing non-specificity of resistance in wheat to head blight caused by inoculation with European strains of Fusarium culmorum, F. graminearum and F. nivale using a multiplicative model for interaction. Theoretical and Applied Genetics 90: 221-228
Utz HF (2000) PLABSTAT: A Computer Program for Statistical Analysis of Plant Breeding Experiments. Version 2N, Institute of Plant Breeding, Seed Science, and Population Genetics, University of Hohenheim, Stuttgart, Germany
Wicklow DT (1981) Interference competition and the organization of fungal communities. In: Wicklow DR and Carrol GC (eds) The Fungal Community (pp 351-375) Marcel Dekker Inc., New York
Zadoks JC, Chang TT and Konzak CF (1974) A decimal code for the growth stages of cereals. Weed Research 14: 415-421
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Miedaner, T., Schilling, A.G. & Geiger Competition Effects Among Isolates of Fusarium culmorum Differing in Aggressiveness and Mycotoxin Production on Heads of Winter Rye. European Journal of Plant Pathology 110, 63–70 (2004). https://doi.org/10.1023/B:EJPP.0000010136.38523.a9
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DOI: https://doi.org/10.1023/B:EJPP.0000010136.38523.a9