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Detection of Murine Intestinal Adenomas Using Targeted Molecular Autofluorescence

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Abstract

There is a significant need for noninvasive methods of evaluating dysplastic and neoplastic lesions in the luminal gastrointestinal tract. We have optimized an approach based on autofluorescence to study dysplastic adenomatous polyps in Apcmin/+ mice. We made recordings from both normal intestinal mucosa and from polyps using a xenon lamp-based fiberoptic device. Seventy-eight polyps in 11 mice revealed an increase in mean autofluorescence intensity ratios of 1.29 ± 0.04 (72 small intestinal polyps; P < 0.0001) and 1.28 ± 0.05 (6 colon polyps; P = 0.0016). Serial measurements of autofluorescence discriminated polyps from normal mucosa with a sensitivity, verified histologically, of 95%. To understand the chemical basis for increased autofluorescence, we examined the tryptophan content of intestinal polyps and the adjacent normal mucosa in a small subset of animals. The findings revealed an increased concentration of tryptophan in polyps (990 ± 240 ng/mg) compared to normal mucosa (720 ± 150 ng/mg; P = 0.03). In conclusion, these findings suggest that autofluorescence intensity increases in the setting of intestinal neoplasia and can be used to detect adenomas in the mouse intestine in real time.

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Authors and Affiliations

  1. Division of Gastroenterology, Department of Medicine, Washington University, Saint Louis, Missouri, USA

    Bhaskar Banerjee, Jeffrey O. Henderson & Nicholas O. Davidson

  2. Department of Computer Science and Engineering, Washington University, Saint Louis, Missouri, USA

    Thomas C. Chaney

Authors
  1. Bhaskar Banerjee
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  2. Jeffrey O. Henderson
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  3. Thomas C. Chaney
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  4. Nicholas O. Davidson
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Banerjee, B., Henderson, J.O., Chaney, T.C. et al. Detection of Murine Intestinal Adenomas Using Targeted Molecular Autofluorescence. Dig Dis Sci 49, 54–59 (2004). https://doi.org/10.1023/B:DDAS.0000011602.02496.76

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  • DOI: https://doi.org/10.1023/B:DDAS.0000011602.02496.76

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