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Effect of Motilin on Endogenous Release of Insulin in Conscious Dogs in the Fed State

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Abstract

The aim was to investigate the insulin-releasing activity of motilin during and after feeding. A single intravenous bolus injection of motilin (0.01–0.3 μg/kg) dose-dependently stimulated endogenous release of insulin in the postprandial state. The insulin-releasing activity of motilin in the fed state was completely abolished by pretreatment with atropine or hexamethonium and was partly inhibited by ondansetron. Truncal vagotomy also greatly suppressed the motilin-induced insulin release. While phentolamine significantly enhanced insulin release in response to motilin, propranolol significantly inhibited this response in both states. The motilin-induced insulin release in the fed states was not accompanied by any changes in glucose concentrations. In conclusion, while the physiological significance remains unclear, these results indicate that physiological doses of motilin stimulate endogenous release of insulin via a vagally cholinergic muscarinic pathway, and that adrenergic and 5-hydroxytryptamine3 receptors are also involved in this response, in the dog.

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Authors and Affiliations

  1. Department of Surgery, Gunma University School of Medicine, 3-39-22 Showamachi, Maebashi, 371-8511, Japan

    Hideki Suzuki, Hiroyuki Kuwano, Erito Mochiki, Norihiro Haga, Tatuo Shimura & Kenichi Nomoto

  2. Institute for Molecular and Cellular Regulation, Gunma University, 3-39-22 Showamachi, Maebashi, 371-8511, Japan

    Toshiyuki Tanaka, Akiyoshi Mizumoto & Zen Itoh

Authors
  1. Hideki Suzuki
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  2. Hiroyuki Kuwano
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  3. Erito Mochiki
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  4. Norihiro Haga
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  5. Tatuo Shimura
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  6. Kenichi Nomoto
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  7. Toshiyuki Tanaka
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  8. Akiyoshi Mizumoto
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  9. Zen Itoh
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Suzuki, H., Kuwano, H., Mochiki, E. et al. Effect of Motilin on Endogenous Release of Insulin in Conscious Dogs in the Fed State. Dig Dis Sci 48, 2263–2270 (2003). https://doi.org/10.1023/B:DDAS.0000007861.91075.b3

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  • DOI: https://doi.org/10.1023/B:DDAS.0000007861.91075.b3

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