Platelet lysate: a replacement for fetal bovine serum in animal cell culture?

Cytotechnology volume 42, Article number: 67 (2003) Cite this article

Abstract

A new cell culture supplement, platelet lysate, was evaluated with reference to fetal bovine serum (FBS), an established industrial medium for animal cell culture. Chemical and bacteriological profiles were conducted including the presence of platelet-derived growth factor (PDGF). PDGF was detected in the platelet lysate but it was not present in FBS. The platelet lysate medium demonstrated lack of microorganisms, mycoplasma and endotoxins. The platelet lysate was investigated in culture studies (cell growth, viability and product formation) towards a number of target cells including myelomas, hybridomas, hepatocytes, fibroblasts and epithelial cells. In general the platelet lysate medium supported cell growth and maintained viabilities comparable or superior to fetal bovine serum. Productivity studies of antibodies (hybridomas) and transferrin (hepatocytes) showed similar or enhanced production in platelet-derived medium in comparison with FBS.

This is a preview of subscription content, log in to check access.

Access options

Buy single article

Instant access to the full article PDF.

US$ 39.95

Tax calculation will be finalised during checkout.

References

  1. Chen Z.L., Xiao C.Z., Liu H., Wu B.C. and Jia X.H. 1996. A novel serum-free medium for the cultivation of vero cells on microcarriers. Biotechnol. Tech. 10(6): 449–452.

    CAS  Google Scholar 

  2. Fredriksson S., Gullberg M., Jarvius J., Olsson C., Pietras K., Gustafsdottir S.M., Ostman A. and Landegren U. 2002. Protein detection using proximity-dependent DNA ligation assays. Nature Biotechnol. 20(5): 473–477.

    Article  CAS  Google Scholar 

  3. Fukimizu H. and Grinell F. 1990. Spatial organization of extracellular matrix and fibroblast activity: effects of serum, transforming growth factor, and fibronectin. Exp. Cell Res. 190: 276–282.

    Article  Google Scholar 

  4. Hodgson J. 1995. To treat or not to treat: that is the question for serum. Biotechnology 13(4): 333–334.

    PubMed  Article  CAS  Google Scholar 

  5. Holmqvist O. and Westermark B. 1989. Blood platelet lysate, method of its preparation and a cell culture medium containing said blood platelet lysate. European Patent Cooperation 413727.

  6. Lebkowski J.S., Schain L.R. and Okarma T.B. 1995. Serum-free culture of hematopoietic stem cells: a review. Stem Cells 13(6): 607–612.

    PubMed  CAS  Article  Google Scholar 

  7. Moreira J.L., Alves P.M., Feliciano A.S., Aunins J.G. and Carrondo M.J.T. 1995. Serum-free and serum-containing media for growth of suspended BHK aggregates in stirred vessels. Enzyme Microbial Technol. 17(5): 437–444.

    Article  CAS  Google Scholar 

  8. Ross R. and Raines E.W. 1990. Platelet-derived growth factor andcellproliferation.In:SaraV.R.(ed.)GrowthFactors:From Genes to Applications, Raven Press, New York, pp. 193–200.

    Google Scholar 

  9. Sporn M.B. and Roberts A.B. 1989. Transforming growth factor-beta: multiple actions and potential applications. J. Am. Med. Assn. 262(7): 938–941.

    Article  CAS  Google Scholar 

  10. Tezel T.H. and Priore L.V.D. 1998. Serum-free media for culturing and serial-passaging of adult human retinal pigment epithelium. Exp. Eye Res. 66(6): 807–815.

    PubMed  Article  CAS  Google Scholar 

Download references

Author information

Affiliations

  1. Department of Chemistry and Biomedical Sciences, University of Kalmar, SE-391 82, Kalmar, Sweden

    Liselott Johansson, Jeanna Klinth & Sten Ohlson

  2. ProLiff AB, SE-392 35, Kalmar, Sweden

    Olov Holmqvist

Authors
  1. Liselott Johansson
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jeanna Klinth
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Olov Holmqvist
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Sten Ohlson
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Sten Ohlson.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Johansson, L., Klinth, J., Holmqvist, O. et al. Platelet lysate: a replacement for fetal bovine serum in animal cell culture?. Cytotechnology 42, 67 (2003). https://doi.org/10.1023/B:CYTO.0000009820.72920.cf

Download citation

  • Animal cell culture
  • Fetal bovine serum
  • Growth supplement
  • Platelet lysate and Roller bottle