Abstract
Effective conservation that preservesevolutionary entities and processes requiresconservation action that is targeted atappropriate taxonomic units. Molecularphylogenetics can contribute to the resolutionof taxonomic uncertainties by defining therelationships between populations and species,and allowing the recognition of taxonomicentities that reflect evolutionary history.Some uncertainty surrounds the status of taxain the Eucalyptus angustissima complex,which includes threatened taxa with restricteddistributions. The phylogenetic relationshipsbetween populations and taxa in the E.angustissima complex were investigated usingnuclear and chloroplast RFLP analysis.Phylogenetic relationships confirmed thegenetic differentiation of two taxa, identifiedspecies level divergence between two taxacurrently recognised at subspecies level, andestablished the identity of a previouslyundetermined population. Phylogeneticrelationships between the taxa were notcongruent with presumed relationships based onmorphological characters alone.
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References
Avise JC (1989) A role for molecular genetics in the recognition and conservation of endangered species. TREE, 4, 279–281.
Avise JC, Walker D (1999) Species realities and numbers in sexual vertebrates: Perspectives from an asexually transmitted genome. Proc. Natl. Acad. Sci. USA, 96, 992–995.
Avise JC, Wollenberg K (1997) Phylogenetics and the origin of species. Proc. Natl. Acad. Sci. USA, 94, 7748–7755.
Brooker MIH, Kleinig DA (1990) Field guide to eucalypts: Volume 2 South-western and southern Australia. Inkata Press, Melbourne.
Byrne M (1999) High genetic identities between three oil mallee taxa, Eucalyptus kochiissp. kochii, ssp. plenissimaand E. horistes, based on nuclear RFLP analysis. Heredity, 82, 205–211.
Byrne M, Macdonald B (2000) Phylogeography and conservation of three oil mallee taxa, Eucalyptus kochiissp kochii, ssp. plenissimaand E. horistes. Aust. J. Bot., 48, 305–312.
Byrne M, Macdonald B, Coates D (2002). Phylogeographic patterns in chloroplast DNA variation within the Acacia acuminata(Leguminosae:Mimosoideae) complex in Western Australia. J. Evol. Biol., 15, 576–587.
Byrne M, Moran GF (1994) Population divergence in the chloroplast genome of Eucalyptus nitens. Heredity, 73, 18–28.
Byrne M, Parrish TL, Moran GF (1998) Nuclear RFLP diversity in Eucalyptus nitens. Heredity, 81, 225–232.
Coates DJ (2000) Defining conservation units in a rich and fragmented flora: Implications for management of genetic resources and evolutionary processes in south-west Australian plants. Aust. J. Bot., 48, 329–339.
Crawford DJ (1989) Enzyme electrophoresis and plant systematics. In: Isozymes in Plant Biology (eds. Soltis DE, Soltis PS), pp. 146–164. Dioscorides Press, Portland, Oregon.
Elliott CP, Byrne M (2004) Genetic diversity within and between natural populations of Eucalyptus occidentalis (Myrtaceae). Silvae Genetica, in press.
Felsenstein J (1993) PHYLIP (Phylogeny inference package) version 3.5c. Department of Genetics, The University of Washington, Seattle, USA.
Goudet J (2001). FSTAT, a program to estimate and test gene diversities and fixation indices (version 2.9.3). Available from http://www.unil.ch/izea/softwares/fstat.html.
Hibbett DS, Donoghue MJ (1996) Implications of phylogenetic studies for conservation of genetic diversity in shiitake mushrooms. Conserv. Biol., 10, 1321–1327.
Hill KD, Johnson LAS (1992) Systematic studies in the eucalypts. 5. New taxa and combinations in Eucalyptus(Myrtaceae) in Western Australia. Telopea, 4(4), 561–634.
Hines B, Byrne M (2001) Some genetic differentiation between mallee and tree forms in the Eucalyptus loxophlebacomplex. Heredity, 87, 566–572.
Jackson HD, Steane DA, Potts BM, Vaillancourt RE (1999) Chloroplast DNA evidence for reticulate evolution in Eucalyptus(Myrtaceae). Mol. Ecol., 8, 739–751.
Nei M (1973) Analysis of gene diversity in subdivided populations. Proceedings of the National Academy of Sciences of the United States of America, 70, 3321–3323.
Nei M (1978) Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics, 89, 583–590.
Rojas M (1992) The species problem and conservation: What are we protecting? Conserv. Biol., 6, 170–178.
Shinozaki K, Ohme M, Tanaka M, Wakasugi T, Hayashiuda N, Matsubayashi T, Zaita N, Chunwongse J, Obokata J, Yamaguchi-Sinosaki K, Ohto C, Torazawa K, Meng BY, Sugita M, Deno H, Kamagashira T, Yamada K, Kusuda J, Takaiwa F, Kato A, Tohdoh N, Shimada H, Sugiura M (1986) The complete nucleotide sequence of the tobacco chloroplast genome: Its gene organization and expression. EMBO J, 5, 2043–2049.
Soltis PS, Gitzendanner MA (1999) Molecular systematics and the conservation of rare species. Conservation Biology, 13, 471–481.
Steane DA, Byrne M, Vaillancourt RE, Potts BM (1998) Chloroplast DNA polymorphisms signal complex inter-specific interactions in Eucalyptus(Myrtaceae). Aust. Syst. Bot., 11, 25–40.
Suguira M, Shinozaki K, Zaita N, Kusuda M, Kumano M (1986) Clone bank of the tobacco (Nicotiana tabacum) chloroplast genome as a set of overlapping restriction endonuclease fragments: Mapping of eleven ribosomal protein genes. Pl. Sci., 44, 211–216.
Swofford DL (1991) PAUP: Phylogenetic Analysis Using Parsimony, Version 3.1.1. Illinois Natural History Survey, Champaign, Illinois.
Sytsma, KJ, Gottlieb LD (1986) Chloroplast DNA evolution and phylogenetic relationships in Clarkiasect. Peripetasma(Onagraceae). Evol., 40, 1248–1261.
Weir BS, Cockerham CC (1984) Estimating F-statistics for the analysis of population structure. Evol., 38, 1358–1370.
Wildy DT, Pate JS, Bartle JR (2000) Variations in composition and yield of leaf oils from alley-farmed oil mallees (Eucalyptusspp.) at a range of contrasting sites in the Western Australian wheatbelt. For. Ecol. Manage., 134, 205–217.
Yates CJ, Hobbs RJ (1997) Temperate eucalypt woodlands: A review of their status, processes threatening their persistence and techniques for restoration. Aust. J. Bot., 45, 949–973.
Yeh FC, Yang RC, Boyle BJ, Ye ZH, Mao JX (1997) POPGENE: The User Friendly Shareware for Population Genetic Analysis. Molecular Biology and Biotechnology Centre, University of Alberta, Canada.
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Elliott, C., Byrne, M. Phylogenetics and the conservation of rare taxa in the Eucalyptus angustissima complex in Western Australia. Conservation Genetics 5, 39–47 (2004). https://doi.org/10.1023/B:COGE.0000014063.56405.93
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DOI: https://doi.org/10.1023/B:COGE.0000014063.56405.93