Conservation Genetics

, Volume 5, Issue 1, pp 39–47 | Cite as

Phylogenetics and the conservation of rare taxa in the Eucalyptus angustissima complex in Western Australia

  • C.P. Elliott
  • M. ByrneEmail author


Effective conservation that preservesevolutionary entities and processes requiresconservation action that is targeted atappropriate taxonomic units. Molecularphylogenetics can contribute to the resolutionof taxonomic uncertainties by defining therelationships between populations and species,and allowing the recognition of taxonomicentities that reflect evolutionary history.Some uncertainty surrounds the status of taxain the Eucalyptus angustissima complex,which includes threatened taxa with restricteddistributions. The phylogenetic relationshipsbetween populations and taxa in the E.angustissima complex were investigated usingnuclear and chloroplast RFLP analysis.Phylogenetic relationships confirmed thegenetic differentiation of two taxa, identifiedspecies level divergence between two taxacurrently recognised at subspecies level, andestablished the identity of a previouslyundetermined population. Phylogeneticrelationships between the taxa were notcongruent with presumed relationships based onmorphological characters alone.

conservation Eucalyptus phylogenetics RFLP 


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  1. Avise JC (1989) A role for molecular genetics in the recognition and conservation of endangered species. TREE, 4, 279–281.Google Scholar
  2. Avise JC, Walker D (1999) Species realities and numbers in sexual vertebrates: Perspectives from an asexually transmitted genome. Proc. Natl. Acad. Sci. USA, 96, 992–995.Google Scholar
  3. Avise JC, Wollenberg K (1997) Phylogenetics and the origin of species. Proc. Natl. Acad. Sci. USA, 94, 7748–7755.Google Scholar
  4. Brooker MIH, Kleinig DA (1990) Field guide to eucalypts: Volume 2 South-western and southern Australia. Inkata Press, Melbourne.Google Scholar
  5. Byrne M (1999) High genetic identities between three oil mallee taxa, Eucalyptus kochiissp. kochii, ssp. plenissimaand E. horistes, based on nuclear RFLP analysis. Heredity, 82, 205–211.Google Scholar
  6. Byrne M, Macdonald B (2000) Phylogeography and conservation of three oil mallee taxa, Eucalyptus kochiissp kochii, ssp. plenissimaand E. horistes. Aust. J. Bot., 48, 305–312.Google Scholar
  7. Byrne M, Macdonald B, Coates D (2002). Phylogeographic patterns in chloroplast DNA variation within the Acacia acuminata(Leguminosae:Mimosoideae) complex in Western Australia. J. Evol. Biol., 15, 576–587.Google Scholar
  8. Byrne M, Moran GF (1994) Population divergence in the chloroplast genome of Eucalyptus nitens. Heredity, 73, 18–28.Google Scholar
  9. Byrne M, Parrish TL, Moran GF (1998) Nuclear RFLP diversity in Eucalyptus nitens. Heredity, 81, 225–232.Google Scholar
  10. Coates DJ (2000) Defining conservation units in a rich and fragmented flora: Implications for management of genetic resources and evolutionary processes in south-west Australian plants. Aust. J. Bot., 48, 329–339.Google Scholar
  11. Crawford DJ (1989) Enzyme electrophoresis and plant systematics. In: Isozymes in Plant Biology (eds. Soltis DE, Soltis PS), pp. 146–164. Dioscorides Press, Portland, Oregon.Google Scholar
  12. Elliott CP, Byrne M (2004) Genetic diversity within and between natural populations of Eucalyptus occidentalis (Myrtaceae). Silvae Genetica, in press.Google Scholar
  13. Felsenstein J (1993) PHYLIP (Phylogeny inference package) version 3.5c. Department of Genetics, The University of Washington, Seattle, USA.Google Scholar
  14. Goudet J (2001). FSTAT, a program to estimate and test gene diversities and fixation indices (version 2.9.3). Available from Scholar
  15. Hibbett DS, Donoghue MJ (1996) Implications of phylogenetic studies for conservation of genetic diversity in shiitake mushrooms. Conserv. Biol., 10, 1321–1327.Google Scholar
  16. Hill KD, Johnson LAS (1992) Systematic studies in the eucalypts. 5. New taxa and combinations in Eucalyptus(Myrtaceae) in Western Australia. Telopea, 4(4), 561–634.Google Scholar
  17. Hines B, Byrne M (2001) Some genetic differentiation between mallee and tree forms in the Eucalyptus loxophlebacomplex. Heredity, 87, 566–572.Google Scholar
  18. Jackson HD, Steane DA, Potts BM, Vaillancourt RE (1999) Chloroplast DNA evidence for reticulate evolution in Eucalyptus(Myrtaceae). Mol. Ecol., 8, 739–751.Google Scholar
  19. Nei M (1973) Analysis of gene diversity in subdivided populations. Proceedings of the National Academy of Sciences of the United States of America, 70, 3321–3323.Google Scholar
  20. Nei M (1978) Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics, 89, 583–590.Google Scholar
  21. Rojas M (1992) The species problem and conservation: What are we protecting? Conserv. Biol., 6, 170–178.Google Scholar
  22. Shinozaki K, Ohme M, Tanaka M, Wakasugi T, Hayashiuda N, Matsubayashi T, Zaita N, Chunwongse J, Obokata J, Yamaguchi-Sinosaki K, Ohto C, Torazawa K, Meng BY, Sugita M, Deno H, Kamagashira T, Yamada K, Kusuda J, Takaiwa F, Kato A, Tohdoh N, Shimada H, Sugiura M (1986) The complete nucleotide sequence of the tobacco chloroplast genome: Its gene organization and expression. EMBO J, 5, 2043–2049.Google Scholar
  23. Soltis PS, Gitzendanner MA (1999) Molecular systematics and the conservation of rare species. Conservation Biology, 13, 471–481.Google Scholar
  24. Steane DA, Byrne M, Vaillancourt RE, Potts BM (1998) Chloroplast DNA polymorphisms signal complex inter-specific interactions in Eucalyptus(Myrtaceae). Aust. Syst. Bot., 11, 25–40.Google Scholar
  25. Suguira M, Shinozaki K, Zaita N, Kusuda M, Kumano M (1986) Clone bank of the tobacco (Nicotiana tabacum) chloroplast genome as a set of overlapping restriction endonuclease fragments: Mapping of eleven ribosomal protein genes. Pl. Sci., 44, 211–216.Google Scholar
  26. Swofford DL (1991) PAUP: Phylogenetic Analysis Using Parsimony, Version 3.1.1. Illinois Natural History Survey, Champaign, Illinois.Google Scholar
  27. Sytsma, KJ, Gottlieb LD (1986) Chloroplast DNA evolution and phylogenetic relationships in Clarkiasect. Peripetasma(Onagraceae). Evol., 40, 1248–1261.Google Scholar
  28. Weir BS, Cockerham CC (1984) Estimating F-statistics for the analysis of population structure. Evol., 38, 1358–1370.Google Scholar
  29. Wildy DT, Pate JS, Bartle JR (2000) Variations in composition and yield of leaf oils from alley-farmed oil mallees (Eucalyptusspp.) at a range of contrasting sites in the Western Australian wheatbelt. For. Ecol. Manage., 134, 205–217.Google Scholar
  30. Yates CJ, Hobbs RJ (1997) Temperate eucalypt woodlands: A review of their status, processes threatening their persistence and techniques for restoration. Aust. J. Bot., 45, 949–973.Google Scholar
  31. Yeh FC, Yang RC, Boyle BJ, Ye ZH, Mao JX (1997) POPGENE: The User Friendly Shareware for Population Genetic Analysis. Molecular Biology and Biotechnology Centre, University of Alberta, Canada.Google Scholar

Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  1. 1.Department of Conservation and Land ManagementScience DivisionBentley Delivery CentreAustralia

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