Abstract
Three bovine cosmid-derived microsatellites (IDV-GA49, IDVGA7 and IDVGA47), previously assigned to cattle syntenic groups U1, U7 and U21, respectively, were fluorescence in situ hybridization (FISH) mapped to river buffalo (Bubalus bubalis, L., 2n= 50) chromosomes (BBU) 3p22 (IDVGA47, U21), BBU 5q21 (IDVGA49, U1) and BBU 5p19 (IDVGA7, U7) using sequential FISH and R-banding techniques. These localizations allowed the assignment, for the first time, of the bovine syntenic groups U21, U1 and U7 to specific river buffalo chromosomes. FISH mapping of IDVGA7 (U7) to cattle rob(1;29) p-arms confirms the banding homologies between BTA 29 and BBU 5p and further supports the idea that cattle standard karyotypes need adjustments.
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References
Committee for Standardized Karyotype of Bubalus bubalis (1994) Standard karyotype of the river buffalo (Bubalus bubalis L., 2n = 50). Report of the committee for the standardization of banded karyotypes of the river buffalo (L. Iannuzzi, coordinator). Cytogenet Cell Genet 67: 102–113.
Di Berardino D, Iannuzzi L, Bettini TM, Matassino D (1981) Ag-NORs variations and banding homologies in two species of Bovidae: Bubalus bubalis L. and Bos taurus L. Can J Genet Cytol 23: 89–99.
Eggen A, Fries R (1995) An integrated cytogenetic and meiotic map of the bovine genome. Anim Genet 26: 215–236.
Eggen A, Oustry A, Vaiman D, Ferretti L, Fries R, Cribiu EP (1994) Bovine syntenic group U7, previously assigned to G-banded chromosome 25, corresponds to R-banded chromosome 29. Hereditas 121: 295–300.
El Nahas SM, Oraby HA, de Hondt HA, Medhat AM, Zahran MM, Mahfouz ER, Kari AM (1996) Synteny mapping in river buffalo. Mamm Genome 7: 831–834.
Gustavsson I (1969) Cytogenetics, distribution and phenotypic effects of a translocation in Swedish cattle. Hereditas 63: 68–169.
Hassaname MS, Chowdhary BP, Gu F, Andersson L, Gustavsson I (1994) Mapping of the interferon gamma (IFNG) gene in river and swamp buffaloes by in situ hybridization. Hereditas 120: 29–33.
Iannuzzi L (1996) G-and R-banded prometaphase karyotypes in cattle (Bos taurus L.) Chrom Res 4: 448–456.
Iannuzzi L, Di Meo GP (1995) Chromosomal evolution in bovids: a comparison of cattle, sheep and goat G-and R-banded chromosomes and cytogenetic divergences among cattle, goat and river buffalo sex chromosomes. Chrom Res 3: 291–299.
Iannuzzi L, Di Meo GP, Perucatti A, Ferrara L (1990a) The high resolution G-and R-banding patterns in chromosomes of river buffalo (Bubalus bubalis L.). Hereditas 112: 209–215.
Iannuzzi L, Di Meo GP, Perucatti A, Ferrara L (1990b) A Comparison of G-and R-banding in cattle and river buffalo prometaphase chromosomes. Caryologia 43: 283–290.
Iannuzzi L, Gallagher DS, Womack JE, Di Meo GP, Skow LC, Ferrara L (1993a) Chromosomal localization of the major histocompatibility complex in cattle and river buffalo by fluorescent in situ hybridization. Hereditas 118: 187–190.
Iannuzzi L, Gallagher DS, Ryan AM, Di Meo GP, Womack JE (1993b) Chromosomal localization of omega and trophoblast interferon genes in cattle and river buffalo by sequential R-banding and fluorescent in situ hybridization. Cytogenet Cell Genet 62: 224–227.
Iannuzzi L, Gallagher DS, Di Meo GP et al. (1993c) Chromosomal localization of the lysozyme gene cluster in river buffalo (Bubalus bubalis L.). Chrom Res 1: 253–255.
Iannuzzi L, Di Meo GP, Ryan AM, Gallagher DS, Ferrara L, Womack JE (1994a) Localization of uridine monophosphate synthase (UMPS) gene to river buffalo chromosomes by FISH. Chrom Res 2: 255–256.
Iannuzzi L, Gallagher DS, Liou LS, Di Meo GP, Ryan AM, Sastry KN, Womack JE (1994b) Chromosomal localization of conglutinin (CGN1) gene to river buffalo by sequential RBH-banding and FISH. Hereditas 120: 283–286.
Iannuzzi L, Gallagher DS, Di Meo GP, Diamond G, Bevins CL, Womack JE (1996a) High-resolution FISH mapping of \gb-defensin genes to river buffalo and sheep chromosomes suggetss a chromosome discrepancy in cattle standard karyotypes. Cytogenet Cell Genet 75: 10–13.
Iannuzzi L, Gallagher DS, Womack JE, Di Meo GP, Schelling CP, Groenen MAM (1996b) FISH mapping of the \ga-S2 casein gene on river buffalo and cattle chromosomes identifies a nomenclature discrepancy in the bovine karyotype. Chrom Res 4: 159–162.
Iannuzzi L, Skow L, Di Meo GP, Gallagher DS, Womack JE (1997) Comparative FISH-mapping of villin (VIL) gene in river buffalo, sheep and goat chromosomes. Chrom Res (in press).
ISCNDA89 (1990) International System for Cytogenetic Nomenclature of Domestic Animals (Di Berardino D, Hayes H, Fries R, Long S, eds). Cytogenet Cell Genet 53: 65–79.
Mezzelani A, Solinas-Toldo S, Nocart M, Guérin G, Ferretti L, Fries R (1994) Mapping of syntenic groups U7 and U27 to bovine chromosomes 25 and 12, respectively. Mamm Genome 5: 574–576.
Mezzelani A, Zhang Y, Redaelli L et al. (1995) Chromosomal localization and molecular characterization of 53 cosmid-derived bovine microsatellites. Mamm Genome 6: 629–635.
Reading Conference (1980)
Texas Nomenclature, Popescu CP, Long S, Riggs P, Womack J, Schmutz S, Fries R, Gallagher D (eds) (1996) Standardization of cattle karyotype nomenclature: report of the committee for the standardization of the cattle karyotype. Cytogenet Cell Genet 74: 259–261.
Threadgill DS, Womack JE (1991) Mapping HSA 3 loci in cattle: additional support for ancestral synteny of HSA 3 and 21. Genomics 11: 1143–1148.
Womack JE, Moll YD (1986) Gene mapping of the cow: conservation of linkage with mouse and man. J Hered 77: 2–7.
Zhang N, Womack JE (1992) Synteny mapping in the bovine: genes from human chromosome 5. Genomics 14: 126–130.
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Iannuzzi, L., Ferretti, L., Di Meo, G.P. et al. FISH Mapping of Bovine U21, U1 and U7 Molecular Markers to River Buffalo Chromosomes 3p, 5q and 5p. Chromosome Res 5, 337–340 (1997). https://doi.org/10.1023/B:CHRO.0000038765.65580.22
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DOI: https://doi.org/10.1023/B:CHRO.0000038765.65580.22