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Chromosome Research

, Volume 12, Issue 3, pp 225–237 | Cite as

Transcriptional repression mechanisms of nucleolus organizer regions (NORs) in humans and chimpanzees

  • Ana Karina Zavala Guillén
  • Yuriko Hirai
  • Tetsuya Tanoue
  • Hirohisa Hirai
Article

Abstract

Polymorphisms related to transcriptional inactivation of nucleolus organizer regions (NORs) have long been described in many animals, particularly humans. However, the precise aetiology of such variations is not always clear. We conducted analyses to investigate the repression mechanisms in humans and chimpanzees using FISH (fluorescence in situ hybridisation) with 18 S rDNA, Ag-NOR (silver nitrate) staining, C-banding, and the in situ nick translation technique with the HpaII restriction enzyme. Examination of 48 humans and 46 chimpanzees suggested that there are at least three different mechanisms that produce inactivation of NORs. These include: (1) elimination of rDNA; (2) DNA methylation; (3) gene silencing due to position effects induced by heterochromatin (C-bands) and/or telomeres.

chimpanzees DNA loss humans methylation NOR inactivation position effect 

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Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Ana Karina Zavala Guillén
    • 1
  • Yuriko Hirai
    • 1
  • Tetsuya Tanoue
    • 2
  • Hirohisa Hirai
    • 1
  1. 1.Primate Research InstituteKyoto University, InuyamaAichiJapan; Tel
  2. 2.Kumamoto Primate Research ParkSanwa Kagaku Kenkyusho Co., Ltd.KumamotoJapan

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