Abstract
An abrin-resistant cell line, CHOR 3-4, was isolated from CHOK1 cells which were resistant to a high concentration of abrin (160 ng/ml), and had a 1000-fold higher resistance to abrin that that of CHOK1 cells.
CHOR 3-4 cells were about 25-fold more resistant than CHOK1 cells to the N-glycosidase activity of abrin, which was measured by hydrolyzing the N-glycosidic bond of adenine 4324 nucleotide from 3′ end of mammalian 28S rRNA. However, the isolated polysomes of CHOR 3-4 cells had the same sensitivity to abrin as those of CHOK1 cells. On measuring the binding of 125I-abrin to CHOR 3-4 cells, it was decreased to about 20% that of CHOK1 cells. This indicates that the mechanism of the resistance of CHOR 3-4 to abrin is due to the alteration of glycoproteins or glycolipids of cell membrane.
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Ko, JL., Lin, JY. Establishment and characterization of an abrin-resistant cell line. Cell Biol Toxicol 13, 75–81 (1997). https://doi.org/10.1023/B:CBTO.0000010392.62109.2c
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DOI: https://doi.org/10.1023/B:CBTO.0000010392.62109.2c