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Filter paper cards contaminated with EMLA cream produce artefacts on acylcarnitine analysis

  • Published:
Journal of Inherited Metabolic Disease

Abstract

Summary: Electrospray ionization tandem mass spectrometry is a widely applied method for the analysis of acylcarnitines in blood samples spotted on filter paper cards (Guthrie cards). When the filter paper cards are contaminated by EMLA cream, highly intense signals at m/z 221 and 235 are detected under ESI-MS/MS conditions, monitoring for precursors of m/z 85. These signals correspond to the active ingredients prilocaine and lidocaine in EMLA and overlap with the signals from the isotopically labelled internal standards (2H3)propionyl carnitine and (2H3)butyrylcarnitine. This interference prevents the proper quantification of the two short-chain acylcarnitines when samples are analysed without derivatization.

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REFERENCE

  • Rogers TL, Ostrow CL (2004) The use of EMLA cream to decrease venipuncture pain in children. J Pediatr Nurs 19: 33–39.

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Author notes

  1. T. Kuster

    Present address: Department of Pediatrics, Division of Clinical Chemistry and Biochemistry, University of Zurich, Steinwiesstrasse 75, 8032, Zurich, Switzerland. E-mail

Authors and Affiliations

  1. Department of Pediatrics, Division of Clinical Chemistry and Biochemistry, University of Zurich, Zurich, Switzerland

    T. Kuster & T. Torresani

  2. Department of Pediatrics, Division of Clinical Chemistry and Biochemistry, University of Zurich, Zurich, Switzerland

    P. Kleinert, S. Durka, F. Neuheiser, C. W. Heizmann & H. Troxler

Authors
  1. T. Kuster
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  2. T. Torresani
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  3. P. Kleinert
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  4. S. Durka
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  5. F. Neuheiser
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  6. C. W. Heizmann
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  7. H. Troxler
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Kuster, T., Torresani, T., Kleinert, P. et al. Filter paper cards contaminated with EMLA cream produce artefacts on acylcarnitine analysis. J Inherit Metab Dis 27, 707–709 (2004). https://doi.org/10.1023/B:BOLI.0000043024.08450.0c

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  • DOI: https://doi.org/10.1023/B:BOLI.0000043024.08450.0c

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