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Surface Effects on PCR Reactions in Multichip Microfluidic Platforms

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Abstract

We evaluated the compatibility of several common plastics, commercially available plastic tubing and disposable syringes which might be useful in the construction of microfluidic platforms with respect to the polymerase chain reaction (PCR). A simple and inexpensive plastic test module was constructed in order to evaluate some of the construction plastics. We also investigated the effect of addition of PEG 8000 to PCR reaction mixtures on the compatibility of materials. These studies identified several common plastics, plastic tubing, and disposable syringes which were compatible with the PCR reaction.

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References

  1. L.J. Kricka and P. Wilding, Microchip PCR. Anal. Bioanal. Chem. 377, 820-825 (2003).

    Google Scholar 

  2. P. Wilding, Biochip Technology, edited by J. Cheng and L.J. Kricka (Harwood Academic Publishers, Philadelphia, 2001), p. 173.

    Google Scholar 

  3. B.C. Giordano, J. Ferrance, S. Swedberg, A.F. Huhmer, and J.P. Landers, Anal. Biochem. 291, 124-132 (2001).

    Google Scholar 

  4. M.A. Shoffner, J. Cheng, G.E. Hvichia, L.J. Kricka, and P. Wilding, Nucleic Acids Res. 24, 375-379 (1996).

    Google Scholar 

  5. N. Zhang, H. Tan, and E.S. Yeung, Anal. Chem. 71, 1138-1145 (1999).

    Google Scholar 

  6. M.A. Northrup, M.T. Ching, R.M. White, and R.T. Watson, Transducers'93, Seventh International Conference on Solid State Sensors and Actuators, Yokohama, Japan, 924-927 (1993).

  7. P. Belgrader, B. Benett, D. Hadley, D. Long, R. Mariella Jr., F. Milanmovich, S. Nasarabadi, W. Nelson, J. Richards, and P. Stratton, Clin. Chem. 44, 2191-2194 (1998).

    Google Scholar 

  8. M.A. Northrup, B. Benett, D. Hadley, P. Landre, S. Lehew, J. Richards J, and P. Stratton, Anal. Chem. 70, 918-922 (1998).

    Google Scholar 

  9. M.U. Kopp, A.J. de Mello, and A. Manz, Science 280, 1046-1048 (1998).

    Google Scholar 

  10. P.K. Yuen, L.J. Kricka, P. Fortina, N.J. Panaro, T. Sakazume, and P. Wilding, Genome Res. 11, 405-412 (2001).

    Google Scholar 

  11. P.A. Marsden, H.H.Q. Heng, S.W. Scherer, R.J. Stewart, A.V. Hall, X.-M. Shi, L.C. Tsui, and K.T. Schappert, J. Biol. Chem. 28, 17478-17488 (1993).

    Google Scholar 

  12. N.J. Panaro, P.K. Yuen, T. Sakazume, P. Fortina, L.J. Kricka, and P. Wilding, Clin. Chem. 46, 1851-1853 (2000).

    Google Scholar 

  13. T.B. Taylor, S.E. Harvey, M. Albin, L. Lebak, Y. Ning, L. Mowat, T. Schuerlein, and E. Principe, J. Biomed. Microdev. 1, 65-70 (1998).

    Google Scholar 

  14. B.C. Giordano, E.R. Copeland, and J.P. Landers, Electrophoresis 22, 334-340 (2001).

    Google Scholar 

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Authors and Affiliations

  1. Department of Pathology and Laboratory Medicine, Hospital of the University of Pennsylvania, 3400 Spruce Street, Founders Pavilion 7.103, Philadelphia, PA, 19104, USA

    Nicholas J. Panaro, Xing Jian Lou, Larry J. Kricka & Peter Wilding

  2. Center for Translational Medicine, Department of Medicine, Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA, 19107, USA

    Paolo Fortina

Authors
  1. Nicholas J. Panaro
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  2. Xing Jian Lou
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  3. Paolo Fortina
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  4. Larry J. Kricka
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  5. Peter Wilding
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Correspondence to Larry J. Kricka.

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Panaro, N.J., Lou, X.J., Fortina, P. et al. Surface Effects on PCR Reactions in Multichip Microfluidic Platforms. Biomedical Microdevices 6, 75–80 (2004). https://doi.org/10.1023/B:BMMD.0000013369.34716.8d

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  • DOI: https://doi.org/10.1023/B:BMMD.0000013369.34716.8d

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