Abstract
The effects of obstructive cholestasis and sex hormones on the total content of prolactin receptor mRNA and ratio of mRNAs of its short and long isoforms have been studied in rat hepatocytes. Obstructive cholestasis caused insignificant changes in total content of prolactin receptor mRNA, but the proportion of mRNA of the long isoform increased. Comparison of prolactin receptor mRNA levels in gonadectomized and intact animals revealed opposite effects of male and female sex hormones on total mRNA, but both groups of these hormones increased the proportion of prolactin receptor short form mRNA. Changes in ratio of mRNA of receptor isoforms found in rat hepatocytes under obstructive cholestasis did not depend on levels of sex hormones. Obstructive cholestasis and sex hormones are suggested to regulate the content of long and short prolactin receptor isoforms in hepatocytes independently.
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REFERENCES
Bole-Feysot, C., Goffln, V., Edery, M., Binart, N., and Kelly, P.A. (1998) Endocrin. Rev.,19, 225–268.
Perrot-Applanat, M., Gualillo, O., Pezet, A., Vincent, V., Edery, M., and Kelly, P.A. (1997) Mol. Endocrinol.,11, 1020–1032.
Das, R., and Vonderhaar, B.K. (1995) Mol. Endocrinol.,9, 1750–1759.
Duan, W.R., Farmer, T.G., Albarracin, C.T., Zhong, L., and Gibori, G. (1997) Endocrinology, 138, 3216–3221.
Bignon, C., Binart, N., Ormandy, C., Schuler, L.A., Kelly, P.A., and Djiane, J. (1997) J. Mol. Endocrinol.,19, 109–120.
Nagano, M., and Kelly, P. (1994) J. Biol. Chem.,269, 13337–13345.
Sakaguchi, K., Ohkubo, T., Sugiyama, T., Tanaka, M., Ushiro, H., and Nakashima, K. (1994) J. Endocrinol.,143, 383–392.
Jolicoeur, C., Boutin, J.M., Okamura, H., Raguet, S., Djiane, J., and Kelly, P.A. (1989) Mol. Endocrinol.,3, 895–890.
Kloehn, S., Otte, C., Korsanke, M., Arendt, T., Clemens, A., Glasow, A., Bornstein, S.R., Folsch, U.R., and Monig, H. (2001) Horm. Metab. Res.,33, 394–401.
Zenkova, T.Yu., Kulikov, A.V., Bogorad, R.L., Rozenkranz, A.A., Platonova, L.V., Shono, N.I., Galperin, E.I., and Smirnova, O.V. (2003) Byul. Eksp. Biol. Med.,135, 664–668.
Garcia-Caballero, T., Mertani, H.M., Lambert, A., Gallego, R., Fraga, M., Pintos, E., Forteza, J., Chevallier, M., Lobie, P.E., Vonderhaar, B.K., Beiras, A., and Morel, G. (2000) Endocrine, 12, 265–271.
Bogorad, R.L., Smyslova, V.S., Rubtsov, P.M., and Smirnova, O.V. (2002) Mol. Biol. (Moscow),36, 91–93.
Alpini, G., Phillips, J.O., Vroman, B., and LaRusso, N.F. (1994) Hepatology, 20, 494–514.
Oleinik, N.V., Smirnova, O.V., and Rozen, V.B. (1990) Probl. Endokrinol.,36, 61–66.
Chomczynski, P., and Sacchi, N. (1987) Analyt. Biochem., 162, 156–159.
Database http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db= Nucleotide.
Pi, X., Zhang, B., Li, J., and Voogt, J.L. (2003) Neuroendocrinology, 77, 187–197.
Smirnova, O., Petraschuk, O., and Kelly, P. (1994) Mol. Cell. Endocrinol.,105, 77–81.
Hu, Z.Z., Li, Z., and Dufau, M.L. (1998) Trends Endocr. Metab.,9, 94–102.
Moldrup, A., Ormandy, C., Nagano, M., Murthy, K., Banville, D., Tronche, F., and Kelly, P.A. (1996) Mol. Endocrinol.,10, 661–671.
Galsgaard, E.D., Nielsen, J.H., and Moldrup, A. (1999) J. Biol.Chem.,274, 18686–18692.
Rozen, V.B., Mataradze, G.D., Smirnova, O.V., and Smirnov, A.N. (1991) Sex Differentiation of Hepatic Functions[in Russian],Meditsina, Moscow.
Janne, O.A., Moilanen, A.M., Poukka, H., Rouleau, N., Karvonen, U., Kotaja, N., Hakli, M., and Palvimo, J.J. (2000) Biochem.Soc.Trans.,28, 401–405.
Webster, N.J.G., and Huang, Z. (1999) in Post transcriptional Processing and the Endocrine System (Chew, S.L., ed.) Karger, Basel Sydney,pp.1–17.
Talamantes, F., and Ortiz, R. (2002) J. Endocrinol.,175, 55–59.
Auboeuf, D., Honig, M., Berget, S.M., and O'Malley, B. W. (2002) Science, 298, 416–419.
Bowen, J.M., Telleria, C.M., Towns, R., and Keyes, P.L. (2000) Eur. J. Endocrinol.,143, 285–292.
Zirulnik, F., Anzulovich, A.C., Larregle, E., Jahn, G. A., and Gimenez, M.S. (2003) Endocr. Res.,29, 201–210.
Pignata, S., Daniele, B., Galati, M.G., Esposito, G., Vallone, P., Fiore, F., Ricchi, P., and Pergola, M. (1997) Eur. J. Gastroenterol. Hepatol.,9, 283–286.
Du, K., Peng, Y., Greenbaum, L.E., Haber, B.A., and Taub, R. (1997) Mol. Cell. Biol.,17, 4096–4104.
Cunningham, B.C., Bass, S., Fuh, G., and Wells, J.A. (1990) Science, 250, 1709–1712.
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Bogorad, R.L., Zenkova, T.Y., Rubtsov, P.M. et al. Influence of Obstructive Cholestasis and Sex Hormones on the Ratio of mRNA of Two Alternative Prolactin Receptor Isoforms in Rat Hepatocytes. Biochemistry (Moscow) 69, 1114–1122 (2004). https://doi.org/10.1023/B:BIRY.0000046885.88590.64
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DOI: https://doi.org/10.1023/B:BIRY.0000046885.88590.64