Abstract
Expression of the rpoS gene encoding the σS subunit of E. coli RNA polymerase was studied on transition to the stationary phase and under conditions of starvation. Polyamines, in particular putrescine, are involved in regulation of the rpoS expression by concentration-dependent stimulation at the levels of translation and stability of the protein σS. The stimulatory effect of exogenous putrescine inversely depended on its content in the cell and was the most pronounced in the beginning of the exponential growth. Possible mechanisms of the polyamine effect on the rpoS expression on post-transcriptional and post-translational levels are discussed.
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REFERENCES
Maeda, H., Fujita, N., and Ishihama, A. (2000) Nucleic Acids Res., 28, 349–3503.
Hengge-Aronis, R. (2002) Microbiol. Mol. Biol. Rev., 66, 373–395.
Tabor, C. W., and Tabor, H. (1985) Microbiol. Rev., 49, 8–99.
Cohen, S. (1978) Nature, 274, 209–210.
Tkachenko, A. G., Salakhetdinova, O. Ya., and Pshenichnov, M. P. (1997) Mikrobiologiya, 67, 329–334.
Tkachenko, A. G., Pshenichnov, M. P., Salakhetdinova, O. Ya., and Nesterova, L. Yu. (1998) Mikrobiologiya, 67, 601–606.
Yoshida, M., Meksuriyen, D., Kashiwagi, K., Kawai, G., and Igarashi, K. (1999) J. Biol. Chem., 274, 22723–22728.
Yoshida, M., Kashiwagi, K., Kawai, G., Ishihama, A., and Igarashi, K. (2001) J. Biol. Chem., 276, 16289–16295. Ref. Type: Journal (Full).
Tkachenko, A., Nesterova, L., and Pshenichnov, M. (2001) Arch. Microbiol., 176, 155–157.
Tkachenko, A. G., and Nesterova, L. Yu. (2003) Biochemistry (Moscow), 68, 850–856.
Jung, I. L., and Kim, I. G. (2003) Biochem. Biophys. Res. Commun.
Yoshida, M., Kashiwagi, K., Kawai, G., Ishihama, A., and Igarashi, K. (2002) J. Biol. Chem., 277, 37139–37146.
Tkachenko, A. G., and Chudinov, A. A. (1989) Mikrobiologiya, 58, 584–590.
Muffler, A., Barth, M., Marschall, C., and Hengge-Aronis, R. (1997) J. Bacteriol., 179, 445–452.
Miller, J. H. (1992) Experiments in Molecular Genetics, Cold Spring Harbor, New York.
Mukhopadhyay, S., Audia, J. P., Roy, R. N., and Schellhorn, H. E. (2000) Mol. Microbiol., 37, 371–381.
Lange, R., and Hengge-Aronis, R. (1994) Genes Dev., 8, 1600–1612.
Lange, R., and Hengge-Aronis, R. (1995) Mol. Microbiol., 5, 49–59.
Muffler, A., Fischer, D., Altuvia, S., Storz, G., and Hengge-Aronis, R. (1996) EMBO J., 15, 1333–1339.
Tkachenko, A. G., and Chudinov, A. A. (1989) Dokl. Akad. Nauk SSSR, 305, 219–222.
Sullivan, K. M., and Lilley, M. J. (1987) J. Mol. Biol., 193, 397–404.
Takayanagi, Y., Tanaka, K., and Takahashi, H. (1994) Mol. Gen. Genet., 243, 525–531.
Pruteanu, M., and Hengge-Aronis, R. (2002) Mol. Microbiol., 45, 1701–1713.
Muffler,A.,Traulsen,D.D.,Lange,R.,and Hengge-Aronis,R.(1996) J.Bacteriol, 178, 1607–1613.
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Tkachenko, A.G., Shumkov, M.S. Role of Putrescine in Regulation of the σS Subunit of RNA Polymerase in Escherichia coli Cells on Transition to Stationary Phase. Biochemistry (Moscow) 69, 876–882 (2004). https://doi.org/10.1023/B:BIRY.0000040219.33023.5a
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DOI: https://doi.org/10.1023/B:BIRY.0000040219.33023.5a