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Elucidation and Characteristics of Non-opioid β-Endorphin Receptors in Rat Adrenal Cortex

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Abstract

β-Endorphin-like decapeptide immunorphin (SLTCLVKGFY), a selective agonist of non-opioid β-endorphin receptor, was labeled with tritium to specific activity of 24 Ci/mmol. It was used for the detection and characterization of nonopioid β-endorphin receptors on rat adrenal cortex membranes (K d1 = 39.6 ± 2.0 nM, Bmax1 = 40.7 ± 2.3 pmol/mg protein; K d2 = 0.25 ± 0.01 μM, Bmax2 = 187.8 ± 9.4 pmol/mg protein). β-Endorphin was found to inhibit the [3H]immunorphin specific binding to membranes (K i = 70.0 ± 9.2 nM); naloxone, [Met5]enkephalin, and α- and γ-endorphins tested in parallel were inactive. Immunorphin at concentrations of 10–9-10–6 M was found to inhibit the adenylate cyclase activity in adrenocortical membranes, while intramuscular injection of immunorphin at doses of 10-100 μg/kg was found to reduce the secretion of 11-oxycorticosteroids from the adrenals to the bloodstream.

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Authors and Affiliations

  1. Branch of Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, pr. Nauki 6, Pushchino, 142290, Russia

    E. V. Navolotskaya, Yu. A. Kovalitskaya, N. V. Malkova & V. M. Lipkin

  2. Institute of Molecular Genetics, Russian Academy of Sciences, pl. Kurchatova 2, Moscow, 123182, Russia

    Yu. A. Zolotarev

  3. Faculty of Biology, Lomonosov Moscow State University, Moscow, 117899, Russia

    N. Yu. Kudryashova & E. N. Goncharenko

  4. State Research Center for Institute of Highly Pure Biopreparations, Ministry of Health of the Russian Federation, Pudozhskaya ul. 7, St. Petersburg, 197110, Russia

    A. A. Kolobov & E. A. Kampe-Nemm

  5. Department of Medicine, University of Maryland School of Medicine, 10 S. Pine St., Baltimore, MD, 21201, USA

    V. V. Yurovsky

Authors
  1. E. V. Navolotskaya
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  2. Yu. A. Kovalitskaya
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  3. Yu. A. Zolotarev
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  4. N. Yu. Kudryashova
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  5. E. N. Goncharenko
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  6. A. A. Kolobov
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  7. E. A. Kampe-Nemm
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  8. N. V. Malkova
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  9. V. V. Yurovsky
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  10. V. M. Lipkin
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Correspondence to E. V. Navolotskaya.

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Navolotskaya, E.V., Kovalitskaya, Y.A., Zolotarev, Y.A. et al. Elucidation and Characteristics of Non-opioid β-Endorphin Receptors in Rat Adrenal Cortex. Biochemistry (Moscow) 69, 870–875 (2004). https://doi.org/10.1023/B:BIRY.0000040218.03078.9f

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  • DOI: https://doi.org/10.1023/B:BIRY.0000040218.03078.9f

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