Stearoyl-poly-N-vinylpyrrolidone (PVP-stear) of various molecular weights (Mn = 1500-5500) self-assemble in aqueous medium. Particles prepared from PVP-stear were characterized in terms of shape and size distribution, and the mechanical stability of the particles was studied. The interaction of PVP-stear and its aggregates with blood components was investigated. Aggregates formed by the polymers with Mn = 1500-3500 in the presence of human serum are stable. The direct lytic action of PVP-stear preparations was studied using sheep and human erythrocytes. The influence of PVP-stear aggregates on the activation of complement system both on classical and alternative pathways was examined. The aggregates prepared from PVP-stear of various molecular weights had no effect on the activation of the complement system.
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Liaw, J., Aoyagi, T., Kataoka, K., Sakurai, Y., and Okano, T. (1998) Pharm. Res., 15, 1721–1726.
Yasugi, K., Nakamura, T., Nagasaki, Y., Kato, M., and Kataoka, K. (1999) J. Macromol., 32, 8024–8032.
Jeong, Y.-I., Nah, J.-W., Lee, Y.-K., Kim, S.-H., and Cho, C.-S. (1999) J. Controll. Rel., 188, 49–58.
Gref, R., Minamitake, Y., Peracchia, M. T., Trubetskoy, V. S., Torchilin, V. P., and Langer, R. (1994) Science, 263, 1600–1603.
Torchilin, V. P. (1988) J. Microencapsulation, 15, 1–19.
Torchilin, V. P. (2001) J. Controll. Rel., 73, 137–172.
Sidelskaya, F. P. (1970) Chemistry of N-Vinylpyrrolidone and Its Polymers [in Russian], Nauka, Moscow.
Shtilman, M. I., Michailova, E. V., Yaroslavov, A. A., and Torchilin, V. P. (1999) Biomaterials, 20, 280–285.
Cohen, S., and Bernstein, H. (1996) in Microparticulate Systems for the Delivery of Proteins and Vaccines (Cohen, S., ed.) Marcel Dekker, New York, pp. 1–49.
Roitt, A. (1991) Essential Immunology [Russian translation], Mir, Moscow.
Williams, C. A., and Chase, M. W. (1976) Methods in Immunology and Immunochemistry, Academic Press, New York.
Torchilin, V. P., Levchenko, T. S., Whiteman, K. R., Yaroslavov, A. A., Tsatsakis, A. M., Rizos, A. K., Michailova, E. V., and Shtilman, M. I. (2001) J. Biomaterials, 22, 3035–3044.
Kozlov, L. V., Krylova, Yu. I., Chikh, V. P., and Molchanova, P. N. (1982) Bioogr. Khim., 8, 652–659.
Raepple, E., Hill, H. U., and Loos, M. (1976) Immunochemistry, 13, 251–255.
Scherbak, I. G., Galebskaya, L. V., and Ryumina, E. V. (1992) RF Patent No. 1532876; Byul. Izobret., 14, 188.
Khalyapin, B. D., and Prokop'ev, A. A. (1986) Immunologiya, 3, 66–69.
Mittel, K. (1980) Micelle Formation, Solubilization and Microemulsions [Russian translation], Mir, Moscow.
Zhang, L., and Eisenberg, A. (1995) Science, 268, 1728–1731.
Tarasova, Yu. V. (2000) Blood Plasma Proteins as Regulators of Hemolysis: Candidate's dissertation [in Russian], St. Petersburg.
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Villemson, A.L., Kuskov, A.N., Shtilman, M.I. et al. Interaction of Polymer Aggregates Based on Stearoyl-poly-N-vinylpyrrolidone with Blood Components. Biochemistry (Moscow) 69, 621–628 (2004). https://doi.org/10.1023/B:BIRY.0000033735.34356.21
- self-assembling systems
- amphiphilic polymer
- colloidal particles
- blood serum
- complement system