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Isolation and Characterization of Site-Specific DNA-methyltransferases from Bacillus coagulans K

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Abstract

Two site-specific DNA methyltransferases, M.BcoKIA and M.BcoKIB, were isolated from the thermophilic strain Bacillus coagulans K. Each of the methylases protects the recognition site 5′-CTCTTC-3′/5′-GAAGAG-3′ from cleavage with the cognate restriction endonuclease BcoKI. It is shown that M.BcoKIB is an N6-adenine specific methylase and M.BcoKIA is an N4-cytosine specific methylase. According to bisulfite mapping, M.BcoKIA methylates the first cytosine in the sequence 5′-CTCTTC-3′.

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Authors and Affiliations

  1. Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Pushchino, 142290, Moscow Region, Russia

    I. V. Svadbina & L. A. Zheleznaya

  2. Institute of Protein Research, Russian Academy of Sciences, Pushchino, 142290, Moscow Region, Russia

    N. V. Zelinskaya, N. P. Kovalevskaya & N. I. Matvienko

Authors
  1. I. V. Svadbina
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  2. N. V. Zelinskaya
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  3. N. P. Kovalevskaya
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  4. L. A. Zheleznaya
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  5. N. I. Matvienko
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Correspondence to N. I. Matvienko.

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Svadbina, I.V., Zelinskaya, N.V., Kovalevskaya, N.P. et al. Isolation and Characterization of Site-Specific DNA-methyltransferases from Bacillus coagulans K. Biochemistry (Moscow) 69, 299–305 (2004). https://doi.org/10.1023/B:BIRY.0000022061.29918.8b

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  • DOI: https://doi.org/10.1023/B:BIRY.0000022061.29918.8b

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