Abstract
The spatial pattern of mitotic activity, cell elongation, rate of H+ fluxes, and 14-3-3 protein content were determined in Zea mays roots. We found that the regions along the apical part of the growing root conversely differ in their proton pumping activity. Higher rate of H+ efflux coincides with higher growth rate and correlates with increased 14-3-3 protein content in membrane preparations. The segment consisting of the root cap and the apical part of the meristem exerts net inward proton pumping, which can be inverted under fusicoccin treatment or osmotic stress. In the latter case, this inversion is accompanied by accumulation of 14-3-3 protein in plasma membranes. The results obtained highlight 14-3-3 protein as an obvious candidate for the fine regulation of plasma membrane H+-ATPase in root apex.
Similar content being viewed by others
REFERENCES
Hager, A., Menzel, H., and Krauss, A. (1971) Planta, 100, 47-75.
Evans, M. L. (1976) Plant Physiol., 58, 599-601.
Pilet, P. E., Versel, J. M., and Mayor, G. (1983) Planta, 158, 398-402.
Zieschang, H. E., Kohler, K., and Sievers, A. (1993) Planta, 190, 546-554.
Peters, W. S., and Felle, H. H. (1999) Plant Physiol., 121, 905-912.
Palmgren, M. G. (1998) Adv. Bot. Res., 28, 1-70.
Morsomme, P., and Boutry, M. (2000) Biochim. Biophys. Acta, 1465, 1-16.
Sze, H., Li, X., and Palmgren, M. G. (1999) Plant Cell, 11, 677-689.
Felle, H. H. (1998) J. Exp. Bot., 49, 987-995.
Parets-Soler, A., Pardo, J. M., and Serrano, R. (1990) Plant Physiol., 93, 1654-1658.
Jahn, T., Baluska, F., Michalke, W., Harper, J. F., and Volkmann, D. (1998) Physiol. Plant., 104, 311-316.
Stenz, H.-G., Heumann, H.-G., and Weisenseel, M. H. (1993) Naturwissenschaften, 80, 317-319.
Aitken, A. (1996) Trends Cell Biol., 6, 341-347.
Oecking, C., and Hagemann, K. (1999) Planta, 207, 480-482.
Jahn, T., Fuglsang, A. T., Olsson, A., Bruntrup, I. M., Collinge, D. B., Volkmann, D., Sommarin, M., Palmgren M. G., and Larsson, C. (1997) Plant Cell, 9, 1805-1814.
Svennelid, F., Olsson, A., Piotrowski, M., Rosenquist, M., Ottman, C., Larsson, C., Oecking, C., and Sommarin, M. (1999) Plant Cell, 11, 2379-2392.
Jelich-Ottmann, C., Weiler, E. W., and Oecking, C. (2001) J. Biol. Chem., 276, 39852-39857.
Chelysheva, V. V., Smolenskaya, I. N., Trofimova, M. S., Babakov, A. V., and Muromtsev, G. S. (1999) FEBS Lett., 456, 22-26.
Babakov, A. V., Chelysheva, V. V., Klychnikov, O. I., Zorinyanz, S. E., Trofimova, M. S., and De Boe, A. H. (2000) Planta, 211, 446-448.
Emi, T., Kinoshita, T., and Shimazaki, K. (2001) Plant Physiol., 125, 1115-1125.
Ivanov, V. B., and Maximov, V. N. (1999) Russ. J. Plant Physiol., 46, 73-82.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall R. J. (1951) J. Biol. Chem., 193, 265-275.
Larsson, C., Sommarin, M., and Widell, S. (1994) Meth. Enzymol., 226, 451-469.
Serrano, R. (1990) in The Plant Plasma Membrane (Larsson, C., and Moller, I. M., eds.) Springer-Verlag, Berlin, pp. 127-153.
Serrano, R. (1996) Int. Rev. Cytol., 165, 1-52.
Erickson, R. O., and Sax, K. B. (1956) Proc. Amer. Phil. Soc., 100, 499-514.
Sentenac, H., and Grignon, C. (1987) Plant Physiol., 84, 1367-1372.
Feldman, L. (1984) Annu. Rev. Plant Physiol., 35, 223-242.
Fasano, J. M., Swanson, S. J., Blancaflor, E. B., Dowd, P. E., Kao, T.-H., and Gilroy, S. (2001) Plant Cell, 13, 907-921.
Korthout, H. A. A. J., and De Boe, A. H. (1998) Plant Physiol. Biochem., 36, 357-365.
Zeleneva, I. V. (1978) Soviet J. Devel. Biol., 8, 244-250.
Camoni, L., Visconti, S., Marra, M., and Aducci, P. (2001) J. Biol. Chem., 276, 31709-31712.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Shanko, A.V., Mesenko, M.M., Klychnikov, O.I. et al. Proton Pumping in Growing Part of Maize Root: Its Correlation with 14-3-3 Protein Content and Changes in Response to Osmotic Stress. Biochemistry (Moscow) 68, 1320–1326 (2003). https://doi.org/10.1023/B:BIRY.0000011653.46422.c3
Issue Date:
DOI: https://doi.org/10.1023/B:BIRY.0000011653.46422.c3