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Recombinant Endo-β-1,4-xylanase from Penicillium canescens

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Abstract

Recombinant endo-β-1,4-xylanase (Xyl-31rec, 31 kD, pI 8.2-9.3, the tenth family of glycosyl hydrolases) was isolated from the culture liquid of Penicillium canescens (strain with the amplified homologous xylanase gene) by chromatofocusing on Mono P and hydrophobic chromatography on phenyl-Superose. It is shown that the biochemical and kinetic parameters, substrate specificity, stability, and other properties of the recombinant and native enzymes are almost the same. It was found that Xyl-31rec can be used for biobleaching of cellulose, the recombinant P. canescens strains providing a high yield of extracellular Xyl-31rec (up to 800-900 U/ml of culture liquid) and not secreting cellulases.

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Authors and Affiliations

  1. Faculty of Chemistry, Lomonosov Moscow State University, Moscow, 199899, Russia

    O. A. Sinitsyna, A. V. Gusakov & A. P. Sinitsyn

  2. Russian Academy of Sciences, Institute of Biochemistry and Physiology of Microorganisms, Pushchino, 142292, Moscow Region, Russia

    O. N. Okunev

  3. Institute of Genetics, Moscow, 113545, Russia

    V. A. Serebryany, E. A. Vavilova & Yu. P. Vinetsky

Authors
  1. O. A. Sinitsyna
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  2. A. V. Gusakov
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  3. O. N. Okunev
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  4. V. A. Serebryany
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  5. E. A. Vavilova
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  6. Yu. P. Vinetsky
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  7. A. P. Sinitsyn
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Sinitsyna, O.A., Gusakov, A.V., Okunev, O.N. et al. Recombinant Endo-β-1,4-xylanase from Penicillium canescens . Biochemistry (Moscow) 68, 1313–1319 (2003). https://doi.org/10.1023/B:BIRY.0000011652.52741.d6

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  • DOI: https://doi.org/10.1023/B:BIRY.0000011652.52741.d6

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