Abstract
The effect of applied arachidonic acid, prostaglandin (PGE1) and various sterols and combinations of arachidonic acid + sterols, on flowering of Pharbitis nil were ascertained by using a tissue culture technique. It was found that arachidonic acid, PGE1 stigmasterol, testosterone, cholesterol, stigmasterol + arachidonic acid, β-sitosterol + arachidonic acid and cholesterol + arachidonic acid all caused earlier flowering. Four inhibitors of prostaglandin biosynthesis (gentisic acid, acetylsalicylic acid, salicylic acid and oleic acid), inhibited flowering completely. The results confirm that the compounds tested could possibly play a role in the flowering of P. nil.
This is a preview of subscription content, log in via an institution to check access.
References
Afzal, M., Ali, M., Hassan, R.A.H., Sweedan, N., Dhami, M.S.I.: Identification of some prostanoids in Aloe vera extracts.-Planta medica 57: 38-40, 1991.
Ali, M., Afzal, M., Hassan, R.A.H., Farid, A., Burka, J.F.: Comparative study of the in vitro synthesis of prostaglandins and thromboxanes in plants belonging to Liliaceae family.-Gen. Pharmacol. 21: 273-276, 1990.
Aukerman, M.J., Amasino, R.M.: Floral induction and florigen.-Cell 93: 491-494, 1998.
Bonner, J., Heftman, E., Zeevaart, J.A.D.: Suppression of floral induction by inhibitors of steroid biosynthesis.-Plant Physiol. 38: 81-88, 1963.
Chailakhyan, M.Kh.: [Concerning the hormonal nature of plant development processes.]-Doklady Akad. Nauk SSSR 16: 227-230, 1937. [In Russ.].
Colasanti, J., Sundaresan, V.: 'Florigen' enters the molecular age: long-distance signals that cause plants to flower.-Trends Biochem. Sci. 25: 236-240, 2000.
De Fossard, R.A.: Flower initiation in tissue and organ cultures.-In: Street, H.E. (ed.): Tissue Culture and Plant Science. Pp. 193-212. Academic Press, London-New York 1974. Evans, L.T.: Daylength and the flowering of plants.-W.A. Benjamin Inc., London-Amsterdam 1975.
Forster, T., Szarvas, T., Tanacs, B.: Plant physiological effects of PGF2 D and prostaglandin synthesis in corn-leaf.-Acta biochem. biophys. Acad. Sci. hung. 19: 42, 1984.
Groenewald, E.G., Van der Westhuizen, A.J.: The flowering stimulus and possible involvement of prostaglandins in the the flowering of Pharbitis nil.-S. Afr. J. Sci. 97: 313-317, 2001.
Groenewald, E.G., Visser, J.H.: The effect of certain inhibitors of prostaglandin biosynthesis on flowering of Pharbitis nil.-Z. Pflanzenphysiol. 71: 67-70, 1974.
Groenewald, E.G., Visser, J.H., Grobbelaar, N.: The occurrence of prostaglandin (PG) F2 D in Pharbitis nil seedlings grown under short-days or long-days.-S. Afr. J. Bot. 2: 82, 1983.
Harada, H.: Flower induction in excised shoot apices of Pharbitis and Chrysanthemum cultured in vitro.-Nature 214: 1027-1028, 1967.
Hempel, F.D., Welch, D.R., Feldman, L.J.: Floral induction and determination: where is flowering controlled?-Trends Plant. Sci. 5: 17-21, 2000.
Hinman, J.W.: Prostaglandins.-Annu. Rev. Biochem. 41: 161-178, 1972.
Lang, A.: Die photoperiodische Regulation von Förderung und Hemmung der Blütenbildung.-Ber. deut. bot. Ges. 97: 293-314, 1984.
Linsmaier, E.M., Skoog, F.: Organic growth factor requirements of tobacco tissue cultures.-Physiol. Plant. 18: 100-127, 1965.
Raghavan, V., Jacobs, W.P.: Studies on the floral histogenesis and physiology of Perilla. II. Floral induction in cultured apical buds of P. frutescens.-Amer. J. Bot. 48: 751-760, 1961.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Groenewald, E., Van Der Westhuizen, A. The Effect of Various Lipids on Flowering of Pharbitis nil in in vitro Culture. Biologia Plantarum 48, 637–639 (2004). https://doi.org/10.1023/B:BIOP.0000047168.78207.21
Issue Date:
DOI: https://doi.org/10.1023/B:BIOP.0000047168.78207.21