Abstract
The culture vessels with multiplying shoots of Achras zapota L. on Schenk and Hildebrandt (SH) medium containing 8.88 μM 6-benzylaminopurine (BAP) with or without sucrose were kept under varied CO2 concentrations ranging from 0.6 to 40.0 g m−3 using different concentrations of sodium bicarbonate (NaHCO3), sodium carbonate (Na2CO3), potassium bicarbonate (KHCO3), and potassium carbonate (K2CO3) in small acrylic chambers. Complete absence of carbon source caused death of shoots within 20 d. Under elevated concentrations of CO2 (10.0 and 40.0 g m−3) the shoots grew photoautotrophically on sucrose-free medium. The growth of cultures was better at 40.0 g (CO2) m−3 than on 3.0 % sucrose under ambient air of growth room. However, the best response was obtained at 10.0 g (CO2) m−3 and 3.0 % sucrose where maximum number of shoots, shoot length, fresh and dry mass, total number of leaves and leaf area was observed.
This is a preview of subscription content, log in via an institution to check access.
References
Aitken-Christie, J., Davies, H., Kubota, C., Kozai, T.: Benefits of using a microporous membrane in tissue culture vessel lid for the micropropagation of Pinus radiata.-In: Van der Plas, L.H.W., Aartrijk, J.V., Nijkamp, H.J.J. (ed.): Progress in Plant Cellular and Molecular Biology: Proceedings of the VII International Congress on Plant Tissue and Cell Culture. Pp. 86. Kluwer Academic Publishers, Amsterdam 1990.
Deng, R., Donnelly, D.J.: In vitro hardening of red raspberry through CO2 enrichment and relative humidity reduction on sugar-free medium.-Can. J. Plant Sci. 73: 1105-1113, 1993.
De Proft, M.P., Maene, L.J., Debergh, P.C.: Carbon dioxide and ethylene evolution in culture atmosphere of Magnolia cultured in vitro.-Physiol. Plant. 65: 375-379, 1985.
Doi, M., Oda, H., Asahira, T.: In vitro atmosphere of cultured C3 and CAM plants in relation to day-lengths.-Environ. Control Biol. 27: 9-13, 1989.
Fujiwara, K., Kozai, T., Watanabe, I.: Fundamental studies on environments in plant tissue culture vessels (3). Measurements of carbon dioxide gas concentration in closed vessels containing tissue cultured plantlets and estimates of net photosynthetic rates of the plantlets.-J. Agr. Meteorol. 43: 21-30, 1987.
Fujiwara, K., Kozai, T., Watanabe, I.: Development of a photoautotrophic tissue culture system for shoot and/or plantlets at rooting and acclimatization stages.-Acta Hort. 230: 153-158, 1988.
Jeong, B.R., Fujiwara, K., Kozai, T.: Carbon dioxide enrichment in autotrophic micropropagation: methods and advantages.-Hort. Technol. 3: 332-334, 1993.
Kirdmanee, C., Kitaya, Y., Kozai, T.: Effect of CO2 enrichment and supporting material in vitro on photoautotrophic growth of Eucalyptus plantlets in vitro and ex vitro.-In vitro cell. dev. Biol. Plant 31: 144-149, 1995.
Kozai, T.: Photoautotrophic micropropagation.-In Vitro cell. dev. Biol. Plant 27: 47-51, 1991.
Kozai, T., Fujiwara, K., Hayashi, M., Aitken-Christie, J.: The in vitro environment and its control in micropropagation.-In: Kurata, K., Kozai, T. (ed.): Transplant Production System. Pp. 247-282. Kluwer Academic Publishers, Dordrecht 1992.
Kozai, T., Iwanami, Y.: Effects of CO2 enrichment and sucrose concentration under high photon fluxes on plantlet growth of carnation (Dianthus caryophyllus L.) in tissue culture during preparation stage.-J. Jap. Soc. Hort. Sci. 57: 279-288, 1988.
Langford, P.J., Wainwright, H.: Effects of sucrose concentration on the photosynthetic ability of rose shoots in vitro.-Ann. Bot. 60: 633-640, 1987.
Morini, S., Melai, M.: CO2 dynamics and growth in photoautotrophic and photomixotrophic apple cultures.-Biol. Plant. 47: 167-172, 2003/4.
Mousseau, M.: CO2 enrichment in vitro: effect on autotrophic and heterotrophic cultures of Nicotiana tabacum.-Photosynth. Res. 8: 187-191, 1986.
Purohit, S.D., Singhvi, A.: Micropropagation of Achras sapota through enhanced axillary branching.-Scientia Hort. 76: 219-229, 1998.
Sha Valli Khan, P.S., Kozai, T., Nguyen, Q.T., Kubota, C., Dhawan, V.: Growth and water relations of Paulownia fortunei under photomixotrophic and photoautotrophic conditions.-Biol. Plant. 46: 161-166, 2003.
Schenk, R.V., Hildebrandt, A.C.: Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures.-Can. J. Bot. 50: 199-204, 1972.
Solárová, J., Pospíšilová, J.: Effect of carbon dioxide enrichment during in vitro cultivation and acclimation to ex vitro conditions.-Biol. Plant. 39: 23-30, 1997.
Solárová, J., Pospíšilová, J., Čatský, J. SantrŮček, J.: Photosynthesis and growth of tobacco plantlets in dependence on carbon supply.-Photosynthetica 23: 629-637, 1989.
Solárová, J., Součková, D., Ullmann, J., Pospíšilová, J.: In vitro culture: environmental conditions and plantlet growth as affected by vessel type and stopper material.-Zahradnictví (Praha) 23: 51-58, 1996.
Thompson, M.R., Thorpe, T.A.: Metabolic and non-metabolic roles of carbohydrates.-In: Bonga, J.M., Durzan, D.J. (ed.): Cell and Tissue Culture in Forestry. Pp. 89-112. Martinus Nijhoff, Dordrecht 1987.
Tichá, I.: Optimization of photoautotrophic tobacco in vitro culture: effect of suncaps closures on plantlet growth.-Photosynthetica 32: 475-479, 1996.
Vasil, I.: Cell Culture and Somatic Cell Genetics of Plants. Vol. 8.-Academic Press, New York 1991.
Vyas, S., Purohit, S.D.: In vitro growth and shoot multiplication of Wrightia tomentosa Roem et Schult in a controlled carbon dioxide environment.-Plant Cell Tissue Organ Cult. 75: 283-286, 2003.
Woltering, E.J.: Ethylene and carbon dioxide accumulation within various tissue culture systems.-Acta. bot. neerl. 35: 50, 1986.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Dave, N., Purohit, S. In vitro Growth and Shoot Multiplication of Achras zapota in a Controlled Carbon Dioxide Environment. Biologia Plantarum 48, 621–624 (2004). https://doi.org/10.1023/B:BIOP.0000047164.06779.27
Issue Date:
DOI: https://doi.org/10.1023/B:BIOP.0000047164.06779.27