Abstract
The effects of different concentrations of NaCl on the activities of antioxidative enzymes in the shoots and roots of soybean (Glycine max [L.] Merr cv. Pershing) inoculated or not with an arbuscular mycorrhizal fungus, Glomus etunicatum Becker & Gerdemann, were studied. Furthermore, the effect of salt acclimated mycorrhizal fungi on the antioxidative enzymes in soybean plants grown under salt stress (100 mM NaCl) was investigated. Activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) were increased in the shoots of both mycorrhizal (M) and nonmycorrhizal (NM) plants grown under NaCl salinity. Salinity increased SOD activity in the roots of M and NM plants, but had no effect on CAT and polyphenol oxidase activities in the roots. M plants had greater SOD, POD and ascorbate peroxidase activity under salinity. Under salt stress, soybean plants inoculated with salt pre-treated mycorrhizal fungi showed increased SOD and POD activity in shoots, relative to those inoculated with the non pre-treated fungi.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Alguacil, M.M., Hernandez, J.A., Caravaca, F., Portillo, B., Roldan, A.: Antioxidant enzyme activities in shoots from three mycorrhizal shrub species afforested in a degraded semi-arid soil.-Physiol. Plant. 118: 562-570, 2003.
Al-Karaki, G.N., Al-Raddad, A.: Effect of arbuscular mycorrhizal fungi and drought stress on growth and nutrient uptake of two wheat genotypes differing in drought resistance.-Mycorrhiza 7: 83-88, 1997.
Al-Karaki, G.N., Hammad, R., Rusan, M.: Response of two tomato cultivars differing in salt tolerance to inoculation with mycorrhizal fungi under salt stress.-Mycorrhiza 11: 43-47, 2001.
Arbona, V., Flors, V., Jacas, J., García-Agustín, P., Gómez-Cadenas, A.: Enzymatic and non-enzymatic antioxidant responses of Carrizo citrange, a salt-sensitive citrus rootstock, to different levels of salinity.-Plant Cell Physiol. 44: 388-394, 2003.
Asada, K.: Production of active oxygen species in photosynthetic tissue.-In: Foyer, C.H., Mullineaux, P.M. (ed.): Causes of Photoxidative Stress and Amelioration of Defense Systems in Plants. Pp. 77-104. CRC, Boca Raton 1994.
Beauchamp, C., Fridovich, I.: Superoxide dismutase: improved assay and an assay applicable to acrylamide gles.-Anal. Biochem. 44: 276-287, 1971.
Benavides, M.P., Marconi, P.L., Gallego, S.M., Comba, M.E., Tomaro, M.L.: Relationship between antioxidant defence systems and salt tolerance in Solanum tuberosum.-Aust. J. Plant Physiol. 27: 273-278, 2000.
Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the preinciple of protein-dye binding.-Anal. Biochem. 72: 248-254, 1976.
Castillo, F.J.: Peroxidases and stress.-In: Penel, C., Gaspar, T., Greppin, H. (ed.): Plant Peroxidases. Topics and Detailed Literature on Molecular, Biochemical, and Physiological Aspects. Pp. 187-203. University of Geneva, Geneva 1992.
Dehne, H.W.: Interaction between vesicular-arbuscular mycorrhizal fungi and plant pathogens.-Phytopathology 72: 1115-1119, 1982.
Dionisio-Sese, M.L., Tobita, S.: Antioxidant responses of rice seedling to salinity stress.-Plant Sci. 135: 1-9, 1998.
Garratt, L.C., Janagoudar, B.S., Lowe, K.C.: Salinity tolerance and antioxidant status in cotton culture.-Free Rad. Biol. Med. 33: 502-511, 2002.
Giannopolitis, C.N., Ries, S.K.: Superoxide dismutases. 1. Occurrence in higher plants.-Plant Physiol. 59: 309-314, 1977.
Gossett, D.R., Millhollon, E.P., Lucas, M.C.: Antioxidant response to NaCl stress in salt tolerant and salt sensitive cultivars of cotton.-Crop Sci. 34: 706-714, 1994.
Harinasut, P., Poonsopa, D., Roengmongkol, K., Charoensataporn, R.: Salinity effects on antioxidant enzymes in mulberry cultivar.-Sci. Asia 29: 109-113, 2003.
Hernandez, J.A., Olmos, E., Corpas, F.J., Sevilla, F., del Rio, L.A.: Salt induced oxidative stress in chloroplasts of pea plants.-Plant Sci. 105: 151-167, 1995.
Hirrel, M.C., Gerdemann, J.W.: Improved growth of onion and bell peppers in saline soils by two vesicular arbuscular mycorrhizal fungi.-Soil Sci. Soc. Amer. J. 44: 654-655, 1980.
Hoagland, D.R., Arnon, D.I.: The water culture method for growing plants without soil.-Calif. Agr. Exp. Sta. Circ. 347: 1-38, 1950.
Imlay, J.A., Linn, S.: DNA damage and oxygen radical toxicity.-Science 240: 1302-1309, 1988.
Jiang, M., Zhang, J.: Effect of abscisic acid on active oxygen species, antioxidative defence system and oxidative damage in leaves of maize seedlings.-Plant Cell Pysiol. 42: 1265-1273, 2001.
Jiang, M., Zhang, J.: Water stress-induced abscisic acid accumulation triggers the increased generation of reactive oxygen species and up-regulates the activities of antioxidant enzymes in maize leaves.-J. exp. Bot. 53: 2401-2410, 2002.
Kar, M., Mishra, D.: Catalase, peroxidase, polyphenol oxidase activities during rice leaf senescence.-Plant Physiol. 57: 315-319, 1976.
Linderman, R.G.: Role of VAM fungi in biocontrol.-In: Pfleger, F.L., Linderman, R.G. (ed.): Mycorrhizae and Plant Health. Pp. 1-27. American Phytopathological Society, St. Paul 1994.
Lopez, F., Vansuyt, G., Casse-Delbart, F., Fourcroy, P.: Ascorbate peroxidase activity, not the mRNA level, is enhanced in salt-stressed Rhaphanus sativus plants.-Physiol. Plant. 97: 13-20, 1996.
Mathur, N., Vyas, A.: Changes in isozyme patterns of peroxidase and polyphenol oxidase by VAM fungi in roots of Ziziphus species.-J. Plant Physiol. 145: 498-500, 1995.
Mathur, N., Vyas, A.: Biochemical changes in Ziziphus xylopyrus by VA mycorrhizae.-Bot. Bull. Acad. sin. 37: 209-212, 1996.
Nakano, Y., Asada, K.: Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts.-Plant Cell Physiol. 22: 867-880, 1981.
Núñez, M., Mazzafera, P., Mazorra, L.M., Siqueira, W.J., Zullo, M.A.T.: Influence of brassinosteroid analogue on antioxidant enzymes in rice grown in culture medium with NaCl.-Biol. Plant. 47: 67-70, 2003/4.
Smirnoff, N.: The role of active oxygen in the response of plants to water deficit and desiccation.-New Phytol. 125: 27-58, 1993.
Smith, S.E., Read, D.J. (ed.): Mycorrhizal Symbiosis. Pp. 105-160.-Academic Press, New York 1995.
Spychalla, J.P., Desbough, S.L.: Superoxide dismutase, catalase, and alpha-tocopherol content of stored potato tubers.-Plant Physiol. 94: 1214-1218, 1990.
Sreenivasulu, N., Ramanjulu, S., Ramachandra-Kini, K., Prakash, H.S., Shekar-Shetty, H., Savithri, H.S., Sudhakar, C.: Total peroxidase activity and peroxidase isoforms as modified by salt stress in two cultivars of fox-tail millet with differential salt tolerance.-Plant Sci. 141: 1-9, 1999.
Sudhakar, C., Lakshmi, A., Giridarakumar, S.: Changes in the antioxidant enzyme efficacy in two high yielding genotypes of mulberry (Morus alba L.) under NaCl salinity.-Plant Sci. 161: 613-619, 2001.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ghorbanli, M., Ebrahimzadeh, H. & Sharifi, M. Effects of NaCl and Mycorrhizal Fungi on Antioxidative Enzymes in Soybean. Biologia Plantarum 48, 575–581 (2004). https://doi.org/10.1023/B:BIOP.0000047157.49910.69
Issue Date:
DOI: https://doi.org/10.1023/B:BIOP.0000047157.49910.69