Abstract
Tobacco (Nicotiana tabacum L. cv. BY-2) cell lines tolerant to 700 μM Ni in which unselected cells can not grow, were selected. The Ni-tolerant cells were also more tolerant to Co, but not to Cd than unselected cells. Ni concentrations in Ni-tolerant cells were always higher than those in medium. Since buthionine sulfoximine did not affect their Ni-tolerance, it is suggested that phytochelatins are not involved in Ni-tolerance of Ni-tolerant cells. On the other hand, histidine contents in Ni-tolerant and unselected cells, which were treated with Ni, were higher that those treated without Ni, and the degree of the elevation of histidine contents by Ni-treatment was higher in Ni-tolerant cells than in unselected cells. Additionally, exogenous histidine reduced the inhibitory effect of Ni on the growth of unselected cells. In addition, the cells that were tolerant to histidine-analogue, had higher contents of histidine and Ni-tolerance. These results suggest that histidine is involved in Ni-tolerance and the detoxification of Ni in symplast in Ni-tolerant cells.
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Nakazawa, R., Kameda, Y., Ito, T. et al. Selection and Characterization of Nickel-Tolerant Tobacco Cells. Biologia Plantarum 48, 497–502 (2004). https://doi.org/10.1023/B:BIOP.0000047143.23646.18
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DOI: https://doi.org/10.1023/B:BIOP.0000047143.23646.18