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Molecular Cloning of a Phosphoenolpyruvate Carboxylase cDNA from Tropical Epiphytic CAM Orchid

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Biologia Plantarum

Abstract

A full-length cDNA encoding phosphoenolpyruvate carboxylase (PEPC) was isolated from tropical epiphytic CAM orchid Mokara Yellow. The cDNA designated as Mpepc1 is 3 450 bp in length with an open reading frame of 2 862 bp encoding 954 amino acids. The deduced amino acid sequence of Mpepc1 shows 83 % identity with pepc2 of sorghum, 82 % with pepc1 and pepc2 of maize and 81 % with pepc of Arabidopsis thaliana. RT-PCR analysis showed that Mpepc1 was expressed in mature leaves, immature leaves, and aerial roots of M. Yellow. No expression was detected in the flower.

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Authors and Affiliations

  1. Department of Biological Sciences, National University of Singapore, 10 Kent Ridge Crescent, Singapore, 119260

    C.R. Li & C.S. Hew

  2. Department of Biological Sciences, National University of Singapore, 10 Kent Ridge Crescent, Singapore, 119260, e-mail

    X.B. Zhang

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  1. C.R. Li
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  2. X.B. Zhang
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  3. C.S. Hew
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Li, C., Zhang, X. & Hew, C. Molecular Cloning of a Phosphoenolpyruvate Carboxylase cDNA from Tropical Epiphytic CAM Orchid. Biologia Plantarum 47, 635–636 (2003). https://doi.org/10.1023/B:BIOP.0000041079.47888.ed

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  • DOI: https://doi.org/10.1023/B:BIOP.0000041079.47888.ed

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