Skip to main content
SpringerLink
Log in

Molecular Cloning of ADP-Glucose Pyrophosphorylase Large Subunit cDNA from Oncidium

  • Published:
Biologia Plantarum

Abstract

A full-length cDNA for ADP-glucose pyrophosphorylase large subunit (AGPL) was isolated from tropical epiphytic orchid Oncidium hybrid Goldiana. The cDNA was 1754 bp in length with an open reading frame of 1551 bp encoding 517 amino acids. The deduced amino acid sequence showed 73 % identity with those of potato isoform 3 (AGPL3) and Arabidopsis thaliana isoform 1 (AGPL1), 71 % identity with that of barley isoform BLPL. RT-PCR analysis showed that AGPL was expressed in mature leaf, immature leaf, developing inflorescence and flower of Oncidium. No expression was detected in roots.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Ainsworth, C., Tarvis, M., Clark, J.: Isolation and analysis of a cDNA clone encoding the small subunit of ADP-glucose pyrophosphorylase from wheat.-Plant mol. Biol. 23: 23–33, 1993.

    Google Scholar 

  • Anderson, J.M., Hnilo, J., Larson, R., Okita, T.W., Morell, M., Preiss, J.: The encoded primary sequence of a rice ADPglucose pyrophosphorylase subunit and its homology to the bacterial enzyme.-J. biol. Chem. 264: 12238–12242, 1989.

    Google Scholar 

  • Bae, J.M., Giroux, M., Hannah, L.: Cloning and characterization of the brittle-2 gene of maize.-Maydica 35:317–322, 1990.

    Google Scholar 

  • Bhave, M.R., Lawrence, S., Barton. C., Hannah, L.C.: Identification and molecular characterization of shrunken-2 cDNA clones of maize.-Plant Cell 2: 581–588, 1990.

    Google Scholar 

  • Giroux. M., Smith-White. B., Gilmore. V., Hannah, L.C., Preiss. J.: The large subunit of the embryo isoform of ADPglucose pyrophosphorylase from maize.-Plant Physiol. 108: 1333–1334. 1995.

    Google Scholar 

  • Harn, C.H., Bac., J.M., Lee, S.S., Min, S.R., Liu, J.R.: Presence of multiple cDNAs encoding an isoform of ADP-glucose pyrophosphorylase large subunit from sweet potato and characterization of expression levels.-Plant Cell Physiol. 41: 1235–1242, 2000.

    Google Scholar 

  • La Cognata, J., Willmitzer, L., Muller-Rober, B.: Molecular cloning and characterization of novel isoforms of potato ADP-glucose pyrophosphorylase.-Mol. gen. Genet. 246: 538–548, 1995.

    Google Scholar 

  • Morell, M.K., Bloom, M., Knowles, V., Preiss, J.: Subunit structure of spinach leaf ADP-glucose pyrophosphorylase.-Plant Physiol. 85: 182–187, 1987.

    Google Scholar 

  • Muller-Rober, B., Kossmann, J., Hannah, L.C., Willmitzer, L., Sonnewald. U.: Only one of two different ADP-glucose pyrophosphorylase genes from potato responds strongly to elevated levels of sucrose.-Mol. gen. Genet. 224: 136–146. 1990.

    Google Scholar 

  • Nakata, P.A., Greene, T.W., Anderson, J.M., Smith-White, B.J., Okita, T.W., Preiss, J.: Comparison of the primary sequences of two potato tuber ADP-glucose pyrophosphorylase subunits.-Plant mol. Biol. 17: 1089–1093. 1991.

    Google Scholar 

  • Okita, T.W., Nakata, P.A., Anderson, J.M., Sowokinos, J., Morell, M., Preiss, J.: The subunit structure of potato tuber ADP-glucose pyrophosphorylase.-Plant Physiol. 93: 785–790, 1990.

    Google Scholar 

  • Olive, M.R., Ellis, R.J., Schuch, W.W.: Isolation and nucleotide sequences of cDNA clones encoding ADP-glucose pyrophosphorylase polypeptides from what leaf and endosperm.-Plant mol. Biol. 12: 525–538. 1989.

    Google Scholar 

  • Park. S.W., Chung. W.I.: Molecular cloning and organ-specific expression of three isoforms of tomato ADP-glucose pyrophosphorylase gene.-Gene 206: 215–221, 1998.

    Google Scholar 

  • Villand, P., Aalen. R., Olsen, O.-A., Luthi, E., onneborg, A., Kleczkowski, L.A.: PCR amplificaton and sequence of cDNA clones for the small and large subunits of ADPglucose pyrophosphorylase from barley tissues.-Plant mol. Biol. 19: 381–389, 1992a.

    Google Scholar 

  • Villand, P., Olsen, O.-A., Kilian, A., Kleczkowski, L.A.: ADPglucose pyrophosphorylase large subunit cI)NA from barley endosperm.-Plant Physiol. 100: 1617–1618, 1992b.

    Google Scholar 

  • Villand, P., Olsen, O.-A., Kleczkowski, L.A.: Molecular characterization of multiple cDNA clones for ADP-glucosc pyrophosphorylase from Arabidopsis thaliana.-Plant mol. Biol. 23: 1279–1284, 1993.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biological Sciences, National University of Singapore, 10 Kent Ridge Crescent, 119260, Singapore, Singapore

    C.R. Li, X.B. Zhang & C.S. Hew

Authors
  1. C.R. Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. X.B. Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. C.S. Hew
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Li, C., Zhang, X. & Hew, C. Molecular Cloning of ADP-Glucose Pyrophosphorylase Large Subunit cDNA from Oncidium . Biologia Plantarum 47, 613–615 (2003). https://doi.org/10.1023/B:BIOP.0000041073.13794.0c

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/B:BIOP.0000041073.13794.0c

Search

Navigation