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Cloning and Expression Analysis of Salt Responsive Gene from Kandelia candel

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Biologia Plantarum

Abstract

Identification of gene expression patterns in mangroves grown under salinity will help to reveal the molecular mechanisms of salt tolerance. Here, 10 cDNAs of genes were isolated from Kandelia candel and identified by representational difference analysis of cDNA (cDNA RDA) under different NaCl concentrations. Of five genes expressed preferentially under salt condition, two were unknown, three were two kinds of low molecular mass heat-shock proteins (sHSPs) and ADP-ribosylation factor, respectively. The expressions of other five genes were repressed under NaCl stress, two encoded cyclophilins, three were tonoplast intrinsic protein, early light-induced protein and 60S ribosomal protein, respectively.

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References

  • Anne, N.S., Sabine, K., Gerd, K., Gunter, F., Bernd. F.: Oxygen stress increases prolyl cus/trans isomerase activity and expression of cyclophilin 18 in rabbit blastocysts.-Biol. Reproduct. 62: 1–7. 2000.

    Google Scholar 

  • Anthony. Y.: Molecular biology of salt tolerance in the context of whole plant physiology. J. exp. Bot. 49: 915–929, 1998.

    Google Scholar 

  • Bowler, L.D., Hubank, M., Spratt. B.G.: Representational difference analysis of cDNA for the detection of differential gene expression in bacteria: development using a model of iron-regulated gene expression in Neisseria meningitidis.-Microbiology 145: 3529–3537, 1999.

    Google Scholar 

  • Collada, C., Gomez, L., Casado, R., Aragoncillo, C.: Purification and in vitro chaperon activity of a class I small heat-shock protein abundant on recalcitrant chestnut seed.-Plant Physiol. 115: 71–77, 1997.

    Google Scholar 

  • Dennis. A.B. Ilene, K.M. Lipman. D.J. Ostell. J., Rapp. B.A. Wheeler. D.L.: GenBank.-Nucleic Acids Res. 28: 15–18. 2000.

    Google Scholar 

  • Elizabeth. J. Farnsworth.. M.F.: Reductions in abscisic acid are linked with viviparous reproduction in mangroves.-Amer. J. Bot. 85: 760–769, 1998.

    Google Scholar 

  • Fang, X.D., Wu, D.G., Lin, Q.F., Li. G.Y.: [A suitable method for RNA extraction of halophytes.]-Nat. Sci. J. Hainan Univ. 16: 311–313, 1998. [In Chinese.]

    Google Scholar 

  • Francois. B., Chaumont. F., Chrispeels, M.J.: High expression of the tonoplast aquaporin ZmTIPI in epidermal and conducting tissues of maize.-Plant Physiol. 117: 1153–1163.1998.

    Google Scholar 

  • Freskgard, P.-O., Bergenhem, N., Jonsson, B.H., Svensson, M., Carlsson, U.: Isomerase and chaperon activity of prolylisomerase in the folding of carbonic anhydrase.-Science 258: 466–468, 1992.

    Google Scholar 

  • Garrett, J.L., Pokala, N., Vierling, E.: Structure and in vitro molecular chaperon activity of cytosolic small heat shock proteins from bean.-J. biol. Chem. 270: 10432–10438, 1995.

    Google Scholar 

  • Garrett, J.L., Roseman, A.M., Saibil, H.R.: A small heat shock protein stably binds heat denatured model substrates and can maintain a substrate in a folding-component state.-EMBO J. 16: 659–671, 1997.

    Google Scholar 

  • Handschumacher, R.E., Harding, M.W., Rice, J., Drugge, R.J.: Cyclophilin: a specific cytosolic binding protein for cyclosporin A.-Science 226: 544–546, 1984.

    Google Scholar 

  • Huang, W., Fang, X.D., Zhao, W.M., Lin, Q.F.: [The methods of identifying differences in mRNA expression.]-Chin. J. Biotechnol. 18: 521–524, 2002. [In Chinese.]

    Google Scholar 

  • Hubank. M. Schatz. D.G.: Identifying differences in mRNA expression by representational difference analysis of cDNA.-Nucl. Acids Res. 22: 5640–5648, 1994.

    Google Scholar 

  • Leonardo, A.M.Z., Baudo, M.M., Palava, E.T., Heino, P.: Isolation and characterization of a eDNA corresponding to a stress-activated cyclophilin gene in Solanum commersonii.-J. exp. Bot. 49: 1451–1452, 1998.

    Google Scholar 

  • Li, M.S., Lee, S.Y.: Mangroves of China: a brief review.-Forest Ecol. Manage. 96: 241–259, 1997.

    Google Scholar 

  • Lin, P.: The physiological characteristics of salt tolerance for Kandelia candel seedlings.-In: Lin, P. (ed.): Mangroves Research papers (11I). Pp. 150–154. Xiamen University Press, Xiamen 1999.

    Google Scholar 

  • Lin, Q.F., Deng, Y.C., Huang, W., Chen, J.P., Li. GY.: [Introducing total DNA of Rhizothora apicularta to Solanum melongena to generate salt-tolerant progenies.]-Progress Biotechnol. 21: 40–44, 2001. [In Chinese.]

    Google Scholar 

  • Maurel, C.: Aquaporins and water permeability of plant membranes.-Annu Rev Plant Physiol Plant mol Biol. 48: 399–429, 1997.

    Google Scholar 

  • Mci, S., Wong, K.C., Lee, J.S.Y.: Reproductive biology and population genetic structure of Kandelia candel (Rhizophoraceae), a viviparous mangrove species.-Amer. J. Bot. 85: 1631–1637, 1998.

    Google Scholar 

  • Sobrado, M.A.: Hydraulic properties of a mangrove Avicennia germinans as affected by NaCI.-Biol. Plant. 44: 435–438, 2001.

    Google Scholar 

  • Stearns, T. Willingham. M.C. Botstein. D., Kahn. R.A.: ADPribosylation factor is functionally and physically associated with the Golgi complex.-Proc. nat. Acad. Sci. USA 87: 1238–1242. 1990

    Google Scholar 

  • lyerman, S.D., Bohnert, H.J., Maurel, C., Steudle, E., Smith, J.A.C.: Plant aquaporins: their molecular biology, biophysics and significance for plant water relations.-J. exp. Bot. 50: 1055–1071, 1999.

    Google Scholar 

  • Weig, A., Deswarte, C., Chrispeels. M.J.: The major intrinsic protein family of Arabidopsis has 23 membranes that form three distinct groups with unctional aquaporins in each group.-Plant Physiol. 114: 1347–1357, 1997.

    Google Scholar 

  • Winicov, I.: New molecular approaches to improving salt tolerance in crop plants.-Ann. Bot. 82: 703–710. 1998.

    Google Scholar 

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Huang, W., Fang, X., Li, G. et al. Cloning and Expression Analysis of Salt Responsive Gene from Kandelia candel . Biologia Plantarum 47, 501–507 (2003). https://doi.org/10.1023/B:BIOP.0000041053.39549.2a

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  • DOI: https://doi.org/10.1023/B:BIOP.0000041053.39549.2a

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