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Regeneration Capacity of Calli Derived from Immature Embryos in Spring Barley Cultivars

  • Published:
Biologia Plantarum

Abstract

Callogenesis, somatic embryogenesis and regeneration capacity in twenty-three agronomically important spring barley (Hordeum vulgare L.) cultivars on induction media with 2,4-dichlorophenoxyacetic acid (2,4-D) or 3,6-dichloro-o-anisic acid (dicamba) and on modified regeneration media were studied. The frequency of zygotic embryos exhibiting callogenesis varied from 88 to 100 % according to genotype. Dicamba was more suitable for somatic embryogenesis induction and exhibited a higher frequency of regenerants than did 2,4-D. Green regenerants were obtained in all cultivars, and there were no albino plants. Except for cv. Victor all cultivars used in the experiment showed lower regeneration capacity as compared to the model cv. Golden Promise.

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Authors and Affiliations

  1. Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Sokolovská 6, CZ-77200, Olomouc, Czech Republic

    E. Halámková, J. Vagera & L. Ohnoutková

  2. Faculty of Sciences, Palacký University, Šlechtitelů 11, CZ-77200, Olomouc, Czech Republic

    J. Vagera

Authors
  1. E. Halámková
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  2. J. Vagera
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  3. L. Ohnoutková
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Correspondence to E. Halámková.

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Halámková, E., Vagera, J. & Ohnoutková, L. Regeneration Capacity of Calli Derived from Immature Embryos in Spring Barley Cultivars. Biologia Plantarum 48, 313–316 (2004). https://doi.org/10.1023/B:BIOP.0000033464.36078.24

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  • DOI: https://doi.org/10.1023/B:BIOP.0000033464.36078.24

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